Visualization of Nucleic Acids

Visualization of Nucleic Acids
Author: Gerard Morel
Publisher: CRC Press
Total Pages: 398
Release: 1995-03-30
Genre: Science
ISBN: 9780849347818

This book presents a review of the principle approaches for visualizing DNA and RNA. Using scanning tunneling and atomic force microscopes, the three-dimensional image of the surface of nucleic acids can be seen at atomic-scale resolutions. Spreading methods provide useful details on structural features of isolated molecules, but the major constituent of living matter is water, and the cryomicroscope makes it possible to look at DNA in its aqueous environment. Genes can be detected simultaneously in situ in chromosomes using fluorescent probes, and also at the electron microscopic level. In cells, nucleic acids are localized and quantified by dyes; electron microscopy is used with cytochemical, immunocytological, nuclease, and in situ hybridization methods. The main potential applications for pathological studies are shown with particular aspects such as viral nucleic acids and in situ PCR.

Principles and Practice of Analytical Techniques in Geosciences

Principles and Practice of Analytical Techniques in Geosciences
Author: Kliti Grice
Publisher: Royal Society of Chemistry
Total Pages: 412
Release: 2014-08-27
Genre: Science
ISBN: 1782623051

The pace of revolution in analytical chemistry in the field of Geosciences has been dramatic over recent decades and includes fundamental developments that have become common place in many related and unrelated disciplines. The analytical tools (nano to macro-scale from stable to radioactive isotopes, compound specific sulfur isotopes) used have been applied to wide-ranging applications from inorganic to organic geochemistry, biodiversity and chronological tools, to build an understanding of how the Earth system evolved to its present state. This book will provide an essential guide to exploring the earth’s natural resources and changing climate by detection science. Individual chapters bring together expertise from across the globe to present a comprehensive outlook on the analytical technologies available to the geoscientist today. Experienced researchers will appreciate the broad treatment of the subject as a valuable reference, while students and those new to the field will quickly gain an appreciation of both the techniques at hand, and the importance of constructing, and analysing, the complex data sets they can generate.

Advanced Imaging and Bio Techniques for Convergence Science

Advanced Imaging and Bio Techniques for Convergence Science
Author: Jun Ki Kim
Publisher: Springer Nature
Total Pages: 560
Release: 2021-04-08
Genre: Medical
ISBN: 981336064X

This book is a wide-ranging guide to advanced imaging techniques and related methods with important applications in translational research or convergence science as progress is made toward a new era in integrative healthcare. Conventional and advanced microscopic imaging techniques, including both non-fluorescent (i.e., label-free) and fluorescent methods, have to date provided researchers with specific and quantitative information about molecules, cells, and tissues. Now, however, the different imaging techniques can be correlated with each other and multimodal methods developed to simultaneously obtain diverse and complementary information. In addition, the latest advanced imaging techniques can be integrated with non-imaging techniques such as mass spectroscopic methods, genome editing, organic/inorganic probe synthesis, nanomedicine, and drug discovery. The book will be of high value for researchers in the biological and biomedical sciences or convergence science who need to use these multidisciplinary and integrated techniques or are involved in developing new analytical methods focused on convergence science.

Principles of Nucleic Acid Structure

Principles of Nucleic Acid Structure
Author: Wolfram Saenger
Publisher: Springer Science & Business Media
Total Pages: 574
Release: 2013-12-01
Genre: Science
ISBN: 1461251907

New textbooks at all levels of chemistry appear with great regularity. Some fields like basic biochemistry, organic reaction mechanisms, and chemical ther modynamics are well represented by many excellent texts, and new or revised editions are published sufficiently often to keep up with progress in research. However, some areas of chemistry, especially many of those taught at the grad uate level, suffer from a real lack of up-to-date textbooks. The most serious needs occur in fields that are rapidly changing. Textbooks in these subjects usually have to be written by scientists actually involved in the research which is advancing the field. It is not often easy to persuade such individuals to set time aside to help spread the knowledge they have accumulated. Our goal, in this series, is to pinpoint areas of chemistry where recent progress has outpaced what is covered in any available textbooks, and then seek out and persuade experts in these fields to produce relatively concise but instructive introductions to their fields. These should serve the needs of one semester or one quarter graduate courses in chemistry and biochemistry. In some cases the availability of texts in active research areas should help stimulate the creation of new courses. CHARLES R. CANTOR New York Preface This monograph is based on a review on polynucleotide structures written for a book series in 1976.

RNA-protein Interactions

RNA-protein Interactions
Author: Kiyoshi Nagai
Publisher: Oxford University Press, USA
Total Pages: 302
Release: 1994
Genre: Medical
ISBN:

The study of RNA-protein interactions is crucial to understanding the mechanisms and control of gene expression and protein synthesis. The realization that RNAs are often far more biologically active than was previously appreciated has stimulated a great deal of new research in this field. Uniquely, in this book, the world's leading researchers have collaborated to produce a comprehensive and current review of RNA-protein interactions for all scientists working in this area. Timely, comprehensive, and authoritative, this new Frontiers title will be invaluable for all researchers in molecular biology, biochemistry and structural biology.

Imaging of Nucleic Acids and Quantitation in Photonic Microscopy

Imaging of Nucleic Acids and Quantitation in Photonic Microscopy
Author: Xavier Ronot
Publisher: CRC Press
Total Pages: 182
Release: 2001-05-30
Genre: Science
ISBN: 1420041703

Visualization of nuclear components has added tremendously to our understanding of the nucleus and its chemistry. The fluorescence approach is considered the present-day standard for comprehending nuclear organization. Imaging of Nucleic Acids and Quantitation in Photonic Microscopy provides new technologies in fluorescence microscopy and image cyt

Hi-C Data Analysis

Hi-C Data Analysis
Author: Silvio Bicciato
Publisher: Humana
Total Pages: 0
Release: 2022-09-04
Genre: Science
ISBN: 9781071613924

This volume details a comprehensive set of methods and tools for Hi-C data processing, analysis, and interpretation. Chapters cover applications of Hi-C to address a variety of biological problems, with a specific focus on state-of-the-art computational procedures adopted for the data analysis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Hi-C Data Analysis: Methods and Protocols aims to help computational and molecular biologists working in the field of chromatin 3D architecture and transcription regulation.

Protein-nucleic Acid Interaction

Protein-nucleic Acid Interaction
Author: Wolfram Saenger
Publisher:
Total Pages: 230
Release: 1989
Genre: Science
ISBN:

This volume contains a series of essays which describe a range of problems in the field of nucleic-acid interactions, investigated by a variety of techniques. An introductory chapter on DNA-protein interactions in the regulation of gene expression is followed by papers on selected model systems.

Diagnostic Molecular Biology

Diagnostic Molecular Biology
Author: Chang-Hui Shen
Publisher: Elsevier
Total Pages: 590
Release: 2023-06-29
Genre: Science
ISBN: 0323986099

Diagnostic Molecular Biology, Second Edition describes the fundamentals of molecular biology in a clear, concise manner with each technique explained within its conceptual framework and current applications of clinical laboratory techniques comprehensively covered. This targeted approach covers the principles of molecular biology, including basic knowledge of nucleic acids, proteins and chromosomes; the basic techniques and instrumentations commonly used in the field of molecular biology, including detailed procedures and explanations; and the applications of the principles and techniques currently employed in the clinical laboratory. Topics such as whole exome sequencing, whole genome sequencing, RNA-seq, and ChIP-seq round out the discussion. Fully updated, this new edition adds recent advances in the detection of respiratory virus infections in humans, like influenza, RSV, hAdV, hRV but also corona. This book expands the discussion on NGS application and its role in future precision medicine. - Provides explanations on how techniques are used to diagnosis at the molecular level - Explains how to use information technology to communicate and assess results in the lab - Enhances our understanding of fundamental molecular biology and places techniques in context - Places protocols into context with practical applications - Includes extra chapters on respiratory viruses (Corona)