Viral Applications of Green Fluorescent Protein

Viral Applications of Green Fluorescent Protein
Author: Barry W. Hicks
Publisher: Humana Press
Total Pages: 384
Release: 2009-04-01
Genre: Science
ISBN: 9781934115879

Over the last ten years, Green Fluorescent Proteins, along with the other spectral variants, have emerged from near obscurity to become a powerful and versatile tool in scientific research. In Viral Applications of Green Fluorescent Protein: Methods and Protocols, leading investigators from around the world contribute detailed examples of both the construction and application of fluorescent proteins delivered by viruses in a format crafted to produce rapid, readily reproducible results. Written in the style of the popular and successful Methods in Molecular BiologyTM series, the chapters include brief introductions to the topics, lists of the necessary materials and reagents, step-by-step laboratory protocols, and Notes sections, which highlight tips on troubleshooting and avoiding known pitfalls. Cutting-edge and easy to use, Viral Applications of Green Fluorescent Protein: Methods and Protocols supplies researchers with an ideal guide to the many uses of GFP and a vital starting point for future studies utilizing this highly adaptable protein.

Viral Applications of Green Fluorescent Protein

Viral Applications of Green Fluorescent Protein
Author: Barry W. Hicks
Publisher: Humana Press
Total Pages: 384
Release: 2009-04-01
Genre: Science
ISBN: 9781934115879

Over the last ten years, Green Fluorescent Proteins, along with the other spectral variants, have emerged from near obscurity to become a powerful and versatile tool in scientific research. In Viral Applications of Green Fluorescent Protein: Methods and Protocols, leading investigators from around the world contribute detailed examples of both the construction and application of fluorescent proteins delivered by viruses in a format crafted to produce rapid, readily reproducible results. Written in the style of the popular and successful Methods in Molecular BiologyTM series, the chapters include brief introductions to the topics, lists of the necessary materials and reagents, step-by-step laboratory protocols, and Notes sections, which highlight tips on troubleshooting and avoiding known pitfalls. Cutting-edge and easy to use, Viral Applications of Green Fluorescent Protein: Methods and Protocols supplies researchers with an ideal guide to the many uses of GFP and a vital starting point for future studies utilizing this highly adaptable protein.

Green Fluorescent Protein

Green Fluorescent Protein
Author: Martin Chalfie
Publisher: John Wiley & Sons
Total Pages: 486
Release: 2005-11-18
Genre: Science
ISBN: 0471739480

Since the discovery of the gene for green fluorescent protein (GFP), derived from jellyfish, this protein that emits a green glow has initiated a revolution in molecular biosciences. With this tool, it is now possible to visualize nearly any protein of interest in any cell or tissue of any species. Since the publication of the first edition, there have been tremendously significant technological advances, including development of new mutant variants. Proteins are now available in yellow and blue, and Novel Fluorescent Proteins (NFPs) have expanded their utility in developing biosensors, biological markers, and other biological applications. This updated, expanded new edition places emphasis on the rise of NFPs, including new chapters on NFP properties with detailed protocols, applications of GFPs and NFPs in industry research, and biosensors. This book provides a solid theoretical framework, along with detailed, practical guidance on use of GFPs and NFPs with discussion of potential pitfalls. The expert contributors provide real examples in showing how to tailor GFP/NFP to specific systems, maximize expression, and enhance detection.

Cell Biology by the Numbers

Cell Biology by the Numbers
Author: Ron Milo
Publisher: Garland Science
Total Pages: 400
Release: 2015-12-07
Genre: Science
ISBN: 1317230698

A Top 25 CHOICE 2016 Title, and recipient of the CHOICE Outstanding Academic Title (OAT) Award. How much energy is released in ATP hydrolysis? How many mRNAs are in a cell? How genetically similar are two random people? What is faster, transcription or translation?Cell Biology by the Numbers explores these questions and dozens of others provid

Illuminating Disease

Illuminating Disease
Author: Marc Zimmer
Publisher: Oxford University Press, USA
Total Pages: 241
Release: 2015
Genre: Medical
ISBN: 0199362815

An illustrated introduction to green fluorescent proteins, one of the most important medical research techniques currently available.

Aglow in the Dark

Aglow in the Dark
Author: Vincent Pieribone
Publisher: Harvard University Press
Total Pages: 306
Release: 2005
Genre: Nature
ISBN: 9780674019218

The discovery of green fluorescent protein revolutionized molecular biology, transforming our study of everything from the AIDS virus to the workings of the brain.

Methods in Plant Cell Biology

Methods in Plant Cell Biology
Author: David W. Galbraith
Publisher: Academic Press
Total Pages: 605
Release: 1995
Genre: Medical
ISBN: 0125641516

Methods in Plant Cell Biology provides in two volumes a comprehensive collection of analytical methods essential for researchers and students in the plant sciences. Individual chapters, written by experts in the field, provide an introductory overview, followed by a step-by-step technical description of the methods. Key Features * Written by experts, many of whom have developed the individual methods described * Contains most, if not all, the methods needed for modern research in plant cell biology * Up-to-date and comprehensive * Full references * Allows quick access to relevant journal articles and to the sources of chemicals required for the procedures * Selective concentration on higher plant methods allows for particular emphasis on those problems specific to plants.

Plant Virology

Plant Virology
Author: Adrian Gibbs
Publisher: Hodder Education
Total Pages: 292
Release: 1980
Genre: Plant viruses
ISBN: 9780713127645

The history and scope of plant virology; Some plant viruses and their names; Effects of viruses on plants; Experimental transmission; The composition and structure of the particles of plant viruses; The purification of virus particles, and some properties of purifield preparations; Infectivity assay; Serological methods; Physical and chemical methods of assay and analysis; The effects of inactivators on virus particles; Behaviour of viruses in plants; Variation, strains and classification; Transmission by vectors and in other natural ways; Virus ecology; Ways of preventing crop losses; Viruses of organisms other than higher plants; Origins of viruses; Plant pathogens confused with viruses.

Skeletal Muscle Development

Skeletal Muscle Development
Author: James G. Ryall
Publisher: Humana Press
Total Pages: 226
Release: 2017-08-25
Genre: Science
ISBN: 9781493972821

This detailed volume presents a wide range of techniques that go beyond the standard assays typically used to assess Myogenesis. The content included addresses assays to analyze skeletal muscle gene expression, proliferating muscle cells, the process of myoblast fusion, muscle development (in vivo), as well as muscle repair. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Skeletal Muscle Development serves as an ideal guide to the study of this highly complex yet carefully regulated process.>