The Effect Of Synthetic Protease Inhibitors On Lysosomal Enzyme Release From Human Neutrophils
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Author | : David L. McCloy |
Publisher | : |
Total Pages | : 140 |
Release | : 1978 |
Genre | : Enzyme inhibitors |
ISBN | : |
Serine esterase activity has been implicated in the neutrophil function of lysosomal enzyme release. To better characterize this esterase role and its possible plurality, the effect of synthetic protease inhibitors on complement- and noncomplement-mediated enzyme secretion was investigated. Noncytotoxic, selective degranulation was achieved by exposing human neutrophils to either 10% Zymosan-Activated Plasma (ZAP) or 0.0001M N-formyl-Methionyl-Phenylalanine (fMP) in the presence of 5 microgram/ml of Cytochalasin B. The majority of the releasing capability generated with ZAP was shown to be C5-related using C5-deficient plasma. Lysosomal markers assayed were Beta-Glucuronidase for primary granule response and Lysozyme for secondary granule involvement. The inhibitors were used to pretreat the cells or were added concomitantly with the releasing stimulus. The tryptic inactivator TLCK inhibited both primary and secondary granule release regardless of the stimulus and inactivation protocol. The chymotryptic inhibitor, TPCK, only blocked fMP-induced release in the absence of inducer; however, when present with either stimulus, secondary granule secretion alone was inhibited. The sulfonyl halide, PMSF, did not impede release under any of the experimental conditions. The chymotrypsin model substrate, BTEE, blocked secondary release by both ZAP and fMP only when present with the secretory stimuli. Its counterpart for tryptic activity, TAME, had no effect on release. These studies suggest the involvement of tryptic and chymotryptic activated esterases in lysosomal enzyme release from human neutrophils.
Author | : |
Publisher | : |
Total Pages | : 1860 |
Release | : 1999 |
Genre | : Medicine |
ISBN | : |
Author | : |
Publisher | : |
Total Pages | : 900 |
Release | : 1979 |
Genre | : Dissertations, Academic |
ISBN | : |
Author | : |
Publisher | : |
Total Pages | : 742 |
Release | : 1989 |
Genre | : Medicine |
ISBN | : |
Author | : |
Publisher | : |
Total Pages | : 368 |
Release | : 1979 |
Genre | : Science |
ISBN | : |
Author | : |
Publisher | : |
Total Pages | : 0 |
Release | : 1971 |
Genre | : |
ISBN | : 9780720440881 |
Author | : |
Publisher | : |
Total Pages | : 746 |
Release | : 1988 |
Genre | : Medicine |
ISBN | : |
Author | : Ralph Snyderman |
Publisher | : Springer Science & Business Media |
Total Pages | : 423 |
Release | : 2013-04-17 |
Genre | : Medical |
ISBN | : 1475748620 |
There was a time, not all that long ago, when scientific study of the cell was called cytology, and the workers in the field named themselves cytologists. When I was a medical student, lectures in cytology were a special, segregated part of the curriculum in the histology course, given along with general anatomy, and they were, as I recall, the surest of cures for insomnia. I still possess Cowdry's three-volume set entitled Special Cytology, published in 1934, and leafing through these books today is rather like examining a medieval manuscript. You could never have guessed what was going to happen to the field. At that time it was all structure, and all guesswork about the structure. When cells were packed together in various tissues, how did the geometry of packing work? How many sides did a liver cell have, in real life? What on earth were all those granules in side, and what were the best stains for looking at them? One thing about those granules, they never moved. Indeed, nothing moved. Cytology turned into cell biology much later on, and suddenly came alive. As has been the case in so many diSciplines in biology, it was brought to life by techniques. New instruments and cytochemical methods were devised for look ing at cells, manipulating cells, more or less in vivo.
Author | : |
Publisher | : |
Total Pages | : 1902 |
Release | : 2003 |
Genre | : Medicine |
ISBN | : |
Vols. for 1963- include as pt. 2 of the Jan. issue: Medical subject headings.
Author | : Wolfgang B. Liedtke, MD, PH.D. |
Publisher | : CRC Press |
Total Pages | : 502 |
Release | : 2006-09-29 |
Genre | : Medical |
ISBN | : 1420005847 |
Since the first TRP ion channel was discovered in Drosophila melanogaster in 1989, the progress made in this area of signaling research has yielded findings that offer the potential to dramatically impact human health and wellness. Involved in gateway activity for all five of our senses, TRP channels have been shown to respond to a wide range of st