The Classic Methods Of Nucleic Acid Visualization For Light Microscopy
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| Author | : Jean-Marie Exbrayat |
| Publisher | : CRC Press |
| Total Pages | : 217 |
| Release | : 2000-11-27 |
| Genre | : Science |
| ISBN | : 1420042513 |
Visualization of nucleic acids has become indispensable to studying cells, tissues, and organisms. Certain techniques even permit quantification of DNA and/or RNA distribution in tissues, but few current analytical books cover the numerous methods for DNA and RNA visualization. This book provides insight into several classic techniques, histologica
| Author | : Gerard Morel |
| Publisher | : CRC Press |
| Total Pages | : 398 |
| Release | : 1995-03-30 |
| Genre | : Science |
| ISBN | : 9780849347818 |
This book presents a review of the principle approaches for visualizing DNA and RNA. Using scanning tunneling and atomic force microscopes, the three-dimensional image of the surface of nucleic acids can be seen at atomic-scale resolutions. Spreading methods provide useful details on structural features of isolated molecules, but the major constituent of living matter is water, and the cryomicroscope makes it possible to look at DNA in its aqueous environment. Genes can be detected simultaneously in situ in chromosomes using fluorescent probes, and also at the electron microscopic level. In cells, nucleic acids are localized and quantified by dyes; electron microscopy is used with cytochemical, immunocytological, nuclease, and in situ hybridization methods. The main potential applications for pathological studies are shown with particular aspects such as viral nucleic acids and in situ PCR.
| Author | : Jean-Marie Exbrayat |
| Publisher | : CRC Press |
| Total Pages | : 216 |
| Release | : 2000-11-27 |
| Genre | : Science |
| ISBN | : 9780849300431 |
Visualization of nucleic acids has become indispensable to studying cells, tissues, and organisms. Certain techniques even permit quantification of DNA and/or RNA distribution in tissues, but few current analytical books cover the numerous methods for DNA and RNA visualization. This book provides insight into several classic techniques, histological as well as histochemical, that can be used to appreciate the nucleic acid status of the cell as well as to provide an overview of RNA and DNA distribution in cells and tissues. Genome Visualization by Classic Methods in Light Microscopy begins with an introduction to DNA and RNA, followed by general visualization principles. The subsequent chapters describe: how to prepare tissues for staining; the principles, chemical formulas, and procedures for nuclear dye, fluorescent dye, and histochemical methods; directions to observe the products of the stained reactions; and more. Each protocol is presented as easy-to-follow directions and the author includes cautionary notes and points to consider. The final section provides color photographs of various tissues in which the staining method, fixative, and observations are noted. A theoretical and practical book, Genome Visualization by Classic Methods in Light Microscopy allows you to understand which technique is most useful for your particular problem. Laboratory protocols are provided for you to follow, chemical structures and principles are provided for you to understand the technique, and the book is organized so you can find the necessary information when needed. This is the essential guide to understanding and executing visualization techniques for nucleic acids.
| Author | : Peter R. Bergethon |
| Publisher | : Springer Science & Business Media |
| Total Pages | : 581 |
| Release | : 2013-03-14 |
| Genre | : Science |
| ISBN | : 1475729634 |
The objective of this book is to provide a unifying approach to the study of biophysical chemistry for the advanced undergraduate who has had a year of physics, organic chem istry, calculus, and biology. This book began as a revised edition of Biophysical Chemistry: Molecules to Membranes, which Elizabeth Simons and I coauthored. That short volume was written in an attempt to provide a concise text for a one-semester course in biophysical chemistry at the graduate level. The experience of teaching biophysical chemistry to bi ologically oriented students over the last decade has made it clear that the subject requires a more fundamental text that unifies the many threads of modem science: physics, chem istry, biology, mathematics, and statistics. This book represents that effort. This volume is not a treatment of modem biophysical chemistry with its rich history and many contro versies, although a book on that topic is also needed. The Physical Basis of Biochemistry is an introduction to the philosophy and practice of an interdisciplinary field in which biological systems are explored using the quantitative perspective of the physical scientist. I have three primary objectives in this volume: one, to provide a unifying picture of the interdisciplinary threads from which the tapestry of biophysical studies is woven; two, to provide an insight into the power of the modeling approach to scientific investigation; and three, to communicate a sense of excitement for the activity and wholesome argument that characterize this field of study.
| Author | : Jean-Marie Exbrayat |
| Publisher | : CRC Press |
| Total Pages | : 339 |
| Release | : 2013-06-05 |
| Genre | : Science |
| ISBN | : 1439822255 |
Histochemistry and cytochemistry are important fields for studying the inner workings of cells and tissues of the body. While visualization techniques have been in use for many years, new methods of detection developed recently help researchers and practitioners better understand cell activity. Histochemical and Cytochemical Methods of Visualizatio
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| Total Pages | : 1626 |
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| Author | : John T. Tansey |
| Publisher | : John Wiley & Sons |
| Total Pages | : 994 |
| Release | : 2020-07-15 |
| Genre | : Science |
| ISBN | : 1119610559 |
Biochemistry: An Integrative Approach with Expanded Topics is addressed to premed, biochemistry, and life science majors taking a two-semester biochemistry course. This version includes all 25 chapters, offering a holistic approach to learning biochemistry. An integrated, skill-focused approach to the study of biochemistry and metabolism Biochemistry integrates subjects of interest to undergraduates majoring in premed, biochemistry, life science, and beyond, while preserving a chemical perspective. Respected biochemistry educator John Tansey takes a unique approach to the subject matter, emphasizing problem solving and critical thinking over rote memorization. Key concepts such as metabolism, are introduced and then revisited and cross-referenced throughout the text to establish pattern recognition and help students commit their new knowledge to long-term memory. As part of WileyPLUS, Biochemistry includes access to video walkthroughs of worked problems, interactive elements, and expanded end-of-chapter problems with a wide range of subject matter and difficulty. Students will have access to both qualitative and quantitative worked problems, and videos model the biochemical reasoning students will need to master. This approach helps students learn to analyze data and make critical assessments of experiments—key skills for success across scientific disciplines. Introduces students in scientific majors to the basics of biochemistry and metabolism Integrates and synthesizes topics throughout the text, allowing students to learn through repetition and pattern recognition Emphasizes problem solving and reasoning skills essential to life sciences, including data analysis and research assessment Provides access to video walkthroughs of worked problems, interactive features, and additional study material through WileyPLUS This volume covers DNA, RNA, gene regulation, synthetic proteins, omics, plant biochemistry, and more. With this text, students studying a range of disciplines are empowered to develop a lasting foundation in biochemistry and metabolism that will serve them as they advance through their careers.
| Author | : Kaijian Xia |
| Publisher | : Frontiers Media SA |
| Total Pages | : 754 |
| Release | : 2022-11-09 |
| Genre | : Science |
| ISBN | : 2832504620 |
| Author | : Xavier Ronot |
| Publisher | : CRC Press |
| Total Pages | : 182 |
| Release | : 2001-05-30 |
| Genre | : Science |
| ISBN | : 1420041703 |
Visualization of nuclear components has added tremendously to our understanding of the nucleus and its chemistry. The fluorescence approach is considered the present-day standard for comprehending nuclear organization. Imaging of Nucleic Acids and Quantitation in Photonic Microscopy provides new technologies in fluorescence microscopy and image cyt
| Author | : Gerard Morel |
| Publisher | : CRC Press |
| Total Pages | : 458 |
| Release | : 1997-02-26 |
| Genre | : Science |
| ISBN | : 9780849326448 |
A multiplicity of methods for visualization are described in Visualization of Receptors. This book of techniques covers immunocytology, radioautography, in situ hybridization, plasmon resonance, RT in vitro PCR, and X-ray crystal structure analysis. Lecturers, researchers, practitioners, technicians, and students will find all the principles and protocols they require and the means of implementing them. Topics discussed include localization of ligands (in vitro or in vivo), visualization of immunoreactivities of ligand and receptor proteins, detection and quantification of mRNA expression, amplification of signals, and determination of 3-D structure. Details of protocols with illustrations of results and commentaries are provided throughout the book.
