Regeneration of the Adult Rat Spinal Cord in Response to Ensheathing Cells and Methylprednisolone

Regeneration of the Adult Rat Spinal Cord in Response to Ensheathing Cells and Methylprednisolone
Author:
Publisher:
Total Pages: 158
Release: 2002
Genre:
ISBN:

Axons fail to regenerate after spinal cord injury (SCI) in adult mammals, leading to permanent loss of function. Following SCI, ensheathing cells promote recovery in animal models, whereas methylprednisolone promotes neurological recovery in humans. The aim of this research was to explore the effectiveness of ensheathing cells and methylprednisolone after acute SCI in the adult rat. Three studies were conducted to accomplish this goal. In the first study, a new method of purifying ensheathing cells was developed, resulting in a final population of ensheathing cells that were 93% pure. In the second study, the ability of a modified directed forepaw reaching (DFR) apparatus to accurately assess function of the corticospinal tract (CST) was examined. The data demonstrated that the modified apparatus prevented extinguishing of DFR behavior after SCI. In addition, the modified apparatus allowed for the collection of quantitative data to accurately assess CST function after bilateral, cervical spinal cord lesions. In the third study, the effectiveness of combining ensheathing cells and methylprednisolone after SCI was investigated. After lesioning the CST in adult rats, a purified population of ensheathing cells was transplanted into the lesion, and methylprednisolone was administered for 24 hours. At six weeks post injury, functional recovery was assessed by measuring successful DFR performance. Axonal regeneration was analyzed by counting the number of anterogradely labeled CST axons caudal to the lesion. Lesioned control rats, receiving either no treatment or vehicle, had abortive axonal regrowth (1 mm) and poor DFR success (38% and 42%, respectively). Compared to controls, rats treated with methylprednisolone for 24 hours had significantly more axons at 7 mm caudal to the lesion, and DFR performance was significantly improved (57%). Rats that received ensheathi.

Indwelling Neural Implants

Indwelling Neural Implants
Author: William M. Reichert
Publisher: CRC Press
Total Pages: 300
Release: 2007-12-17
Genre: Medical
ISBN: 1420009303

Despite enormous advances made in the development of external effector prosthetics over the last quarter century, significant questions remain, especially those concerning signal degradation that occurs with chronically implanted neuroelectrodes. Offering contributions from pioneering researchers in neuroprosthetics and tissue repair, Indwel

Model Organisms in Spinal Cord Regeneration

Model Organisms in Spinal Cord Regeneration
Author: Catherina G. Becker
Publisher: John Wiley & Sons
Total Pages: 423
Release: 2007-03-30
Genre: Medical
ISBN: 3527610359

This handbook provides a comprehensive overview for students, clinicians and researchers planning to enter the field of neural regeneration, combining the latest knowledge with an understanding of all important model organisms in one handy volume. By covering the strengths and weaknesses as well as possible applications of different models it saves researchers both time and resources in their choice of the appropriate model organism. An equally valuable introduction for the novice planning to enter the field.

Spinal Cord Injury

Spinal Cord Injury
Author: Institute of Medicine
Publisher: National Academies Press
Total Pages: 360
Release: 2005-07-27
Genre: Medical
ISBN: 0309165202

An estimated 11,000 spinal cord injuries occur each year in the United States and more than 200,000 Americans suffer from maladies associated with spinal cord injury. This includes paralysis, bowel and bladder dysfunction, sexual dysfunction, respiratory impairment, temperature regulation problems, and chronic pain. During the last two decades, longstanding beliefs about the inability of the adult central nervous system to heal itself have been eroded by the flood of new information from research in the neurosciences and related fields. However, there are still no cures and the challenge of restoring function in the wake of spinal cord injuries remains extremely complex. Spinal Cord Injury examines the future directions for research with the goal to accelerate the development of cures for spinal cord injuries. While many of the recommendations are framed within the context of the specific needs articulated by the New York Spinal Cord Injury Research Board, the Institute of Medicine's panel of experts looked very broadly at research priorities relating to future directions for the field in general and make recommendations to strengthen and coordinate the existing infrastructure. Funders at federal and state agencies, academic organizations, pharmaceutical and device companies, and non-profit organizations will all find this book to be an essential resource as they examine their opportunities.

Textbook of Neural Repair and Rehabilitation: Volume 1, Neural Repair and Plasticity

Textbook of Neural Repair and Rehabilitation: Volume 1, Neural Repair and Plasticity
Author: Michael Selzer
Publisher: Cambridge University Press
Total Pages: 693
Release: 2014-04-24
Genre: Medical
ISBN: 1139915959

In two freestanding volumes, the Textbook of Neural Repair and Rehabilitation provides comprehensive coverage of the science and practice of neurological rehabilitation. Revised throughout, bringing the book fully up to date, this volume, Neural Repair and Plasticity, covers the basic sciences relevant to recovery of function following injury to the nervous system, reviewing anatomical and physiological plasticity in the normal central nervous system, mechanisms of neuronal death, axonal regeneration, stem cell biology, and research strategies targeted at axon regeneration and neuron replacement. New chapters have been added covering pathophysiology and plasticity in cerebral palsy, stem cell therapies for brain disorders and neurotrophin repair of spinal cord damage, along with numerous others. Edited and written by leading international authorities, it is an essential resource for neuroscientists and provides a foundation for the work of clinical rehabilitation professionals.

Spinal Cord Injury (SCI) Repair Strategies

Spinal Cord Injury (SCI) Repair Strategies
Author: Giuseppe Perale
Publisher: Woodhead Publishing
Total Pages: 346
Release: 2019-10-30
Genre: Medical
ISBN: 0081028083

Spinal Cord Injury (SCI) Repair Strategies provides researchers the latest information on potential regenerative approaches to spinal cord injury, specifically focusing on therapeutic approaches that target regeneration, including cell therapies, controlled drug delivery systems, and biomaterials. Dr. Giuseppe Perale and Dr. Filippo Rossi lead a team of authoritative authors in academia and industry in this innovative reference on the field of regenerative medicine and tissue engineering. This book presents all the information readers need to understand the current and potential array of techniques, materials, applications and their benefits for spinal cord repair. Covers current and future repair strategies for spinal cord injury repair Focuses on key research trends, clinics, biology and engineering Provides fundamentals on regenerative engineering and tissue engineering

The Spinal Cord

The Spinal Cord
Author: Charles Watson
Publisher: Academic Press
Total Pages: 408
Release: 2009-11-27
Genre: Medical
ISBN: 0080921388

Many hundreds of thousands suffer spinal cord injuries leading to loss of sensation and motor function in the body below the point of injury. Spinal cord research has made some significant strides towards new treatment methods, and is a focus of many laboratories worldwide. In addition, research on the involvement of the spinal cord in pain and the abilities of nervous tissue in the spine to regenerate has increasingly been on the forefront of biomedical research in the past years. The Spinal Cord, a collaboration with the Christopher and Dana Reeve Foundation, is the first comprehensive book on the anatomy of the mammalian spinal cord. Tens of thousands of articles and dozens of books are published on this subject each year, and a great deal of experimental work has been carried out on the rat spinal cord. Despite this, there is no comprehensive and authoritative atlas of the mammalian spinal cord. Almost all of the fine details of spinal cord anatomy must be searched for in journal articles on particular subjects. This book addresses this need by providing both a comprehensive reference on the mammalian spinal cord and a comparative atlas of both rat and mouse spinal cords in one convenient source. The book provides a descriptive survey of the details of mammalian spinal cord anatomy, focusing on the rat with many illustrations from the leading experts in the field and atlases of the rat and the mouse spinal cord. The rat and mouse spinal cord atlas chapters include photographs of Nissl stained transverse sections from each of the spinal cord segments (obtained from a single unfixed spinal cord), detailed diagrams of each of the spinal cord segments pictured, delineating the laminae of Rexed and all other significant neuronal groupings at each level and photographs of additional sections displaying markers such as acetylcholinesterase (AChE), calbindin, calretinin, choline acetlytransferase, neurofilament protein (SMI 32), enkephalin, calcitonin gene-related peptide (CGRP), and neuronal nuclear protein (NeuN). The text provides a detailed account of the anatomy of the mammalian spinal cord and surrounding musculoskeletal elements The major topics addressed are: development of the spinal cord; the gross anatomy of the spinal cord and its meninges; spinal nerves, nerve roots, and dorsal root ganglia; the vertebral column, vertebral joints, and vertebral muscles; blood supply of the spinal cord; cytoarchitecture and chemoarchitecture of the spinal gray matter; musculotopic anatomy of motoneuron groups; tracts connecting the brain and spinal cord; spinospinal pathways; sympathetic and parasympathetic elements in the spinal cord; neuronal groups and pathways that control micturition; the anatomy of spinal cord injury in experimental animals The atlas of the rat and mouse spinal cord has the following features: Photographs of Nissl stained transverse sections from each of 34 spinal segments for the rat and mouse; Detailed diagrams of each of the 34 spinal segments for rat and mouse, delineating the laminae of Rexed and all other significant neuronal groupings at each level. ; Alongside each of the 34 Nissl stained segments, there are additional sections displaying markers such as acetylcholinesterase, calbindin, calretinin, choline acetlytransferase, neurofilament protein (SMI 32), and neuronal nuclear protein (NeuN) All the major motoneuron clusters are identified in relation to the individual muscles or muscle groups they supply

Degeneration and Regeneration in the Nervous System

Degeneration and Regeneration in the Nervous System
Author: Norman Saunders
Publisher: CRC Press
Total Pages: 354
Release: 2003-09-02
Genre: Medical
ISBN: 0203304489

Degeneration and Regeneration in the Nervous System brings together an international team of contributors to produce a series of critical reviews appraising key papers in the field. The pace of research on brain and spinal cord injury quickened considerably in the last ten years and there is much that is new and important that is covered in this book. However, there is still a long way to go before our knowledge will explain fully why the central nervous system has such a limited capacity for regeneration, and before experimental solutions can be applied to the patient. With emphasis on actual and therapeutic importance of the work reviewed, Degeneration and Regeneration in the Nervous System is a useful overview for graduate students, their teachers and researchers working in this field.

Regenerative Biology and Medicine

Regenerative Biology and Medicine
Author: David L. Stocum
Publisher: Elsevier
Total Pages: 460
Release: 2010-07-26
Genre: Science
ISBN: 0080493025

The purpose of the book is to bring together in one place the different facets of regenerative biology and medicine while providing the reader with an overview of the basic and clinically-oriented research that is being done. Not only does the content cover a plethora tissues and systems, it also includes information about the developmental plasticity of adult stem cells and the regeneration of appendages.As part of its balanced presentation, Regenerative Biology and Medicine does address the biological/bioethical issues and challanges involved in the new and exciting field of regenerative biology and medicine. *Tissues covered include skin, hair, teeth, cornea, and central neural types*Systems presented are digestive, respiratory, urogenital, musculoskeletal, and cardiovascular*Includes amphibians as powerful research models*Discusses appendage regeneration in amphibians and mammals