Protein Lipidation

Protein Lipidation
Author: Maurine E. Linder
Publisher: Humana
Total Pages: 327
Release: 2019-06-01
Genre: Science
ISBN: 9781493995318

This volume explores techniques used to detect lipids attached to proteins, to analyze the function of lipid modifications, and to characterize the enzymes that add and remove lipids from proteins. The book is organized into seven parts: Part One describes chemically-based strategies to identify substrates for protein lipidation that can be applied to individual proteins or globally using proteomics. Part Two focuses on the enzymes that remove fatty acids from proteins and provides methods to monitor protein biogenesis and palmitate turnover. Part Three addresses biochemical and cellular characterization of DHHC S-acyltransferases, a family of enzymes with 23 members encoded by the human genome. Part Four presents the SwissPalm 2 database and tips on how to use it effectively. Part Five focuses on fatty acylation that occurs in the lumen of the secretory pathway. Parts Six and Seven conclude the book with methods to produce and assay lipid-modified and integral membrane proteins. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and authoritative, Protein Lipidation: Methods and Protocols is a valuable resource for experts in the field and for investigators who encounter protein lipidation through their research on a particular cellular process or favorite protein.

Protein Lipidation Protocols

Protein Lipidation Protocols
Author: Michael H. Gelb
Publisher: Springer Science & Business Media
Total Pages: 243
Release: 2008-02-03
Genre: Science
ISBN: 1592592643

In Protein Lipidation Protocols, Michael Gelb brings together a collection of readily reproducible techniques for studying protein lipidation, the covalent attachment of lipids to proteins. These cutting-edge methods-many never published before in a "hands-on" format-deal with glycosyl phosphatidylinositol (GPI)-containing compounds, protein fatty acylation, and protein prenylation. Included are novel techniques for determining the chemical structure of GPI-anchors, for radiolabeling the prenyl groups of protein in eukaryotic cells, a tool for developing inhibitors of the protein farnesyltransferase, and for an exciting lysosomal enzyme that cleaves fatty acyl groups from proteins, the first fatty acylase discovered. Protein Lipidation Protocols offers biochemists, cell and molecular biologists, medicinal chemists, and pharmaceutical researchers state-of-the-art tools for understanding the complex biochemistry of protein lipidation, as well as catalyzing the development of many important new biopharmaceuticals, including anticancer drugs.

Protein Lipidation

Protein Lipidation
Author:
Publisher: Elsevier
Total Pages: 349
Release: 2000-10-19
Genre: Science
ISBN: 0080542433

This is the first compilation of protein lipidation enzymes. This volume summarizes recent dramatic developments regarding enzymes responsible for protein lipidation, a process critical for a number of physiological functions, including cell proliferation and morphology. Inhibitors of protein lipidation have recently been shown to be useful as anticancer drugs. Enzymatic mechanisms, mutational analysis, and structural studies are presented. - The enzymatic mechanisms of protein lipidation - Three-dimensional structures of protein farnesytransferase, protein geranylgeranytransferase II, and n-myristoryltransferase

Biogenesis of Fatty Acids, Lipids and Membranes

Biogenesis of Fatty Acids, Lipids and Membranes
Author: Otto Geiger
Publisher: Springer
Total Pages: 0
Release: 2019-01-29
Genre: Science
ISBN: 9783319504292

Concise chapters, written by experts in the field, cover a wide spectrum of topics on lipid and membrane formation in microbes (Archaea, Bacteria, eukaryotic microbes).All cells are delimited by a lipid membrane, which provides a crucial boundary in any known form of life. Readers will discover significant chapters on microbial lipid-carrying biomolecules and lipid/membrane-associated structures and processes.

Posttranslational Modification of Proteins

Posttranslational Modification of Proteins
Author: Christopher Walsh
Publisher: Roberts and Company Publishers
Total Pages: 524
Release: 2006
Genre: Science
ISBN: 9780974707730

Covering the major classes of posttranslational modifications, Posttranslational Modification of Proteins is the first comprehensive treatment of this burgeoning area of proteome diversification.

Membrane Biogenesis

Membrane Biogenesis
Author: Jos A.F. Op den Kamp
Publisher: Springer Science & Business Media
Total Pages: 474
Release: 2013-06-29
Genre: Science
ISBN: 3642731848

Many individual aspects of the dynamics and assembly of biological membranes have been studied in great detail. Cell biological approaches, advanced genetics, biophysics and biochemistry have greatly contributed to an increase in our knowledge in this field.lt is obvious however, that the three major membrane constituents - lipids, proteins and carbohydrates- are studied, in most cases separately and that a coherent overview of the various aspects of membrane biogenesis is not readily available. The NATO Advanced Study Institute on "New Perspectives in the Dynamics of Assembly of Biomembranes" intended to provide such an overview: it was set up to teach students and specialists the achievements obtained in the various research areas and to try and integrate the numerous aspects of membrane assembly into a coherent framework. The articles in here reflect this. Statting with detailed contributions on phospholipid structure, dynamics, organization and biogenesis, an up to date overview of the basic, lipidic backbone of biomembranes is given. Extensive progress is made in the research on membrane protein biosynthesis. In particular the post- and co-translational modification processes of proteins, the mechanisms of protein translocation and the sorting mechanisms which are necessary to direct proteins to their final, intra - or extracellular destination have been characterized in detail. Modern genetic approaches were indispensable in this research area: gene cloning, hybrid protein construction, site directed mutagenesis and sequencing techniques elucidated many functional aspects of specific nucleic acid and amino acid sequences.

Long-lived Proteins in Human Aging and Disease

Long-lived Proteins in Human Aging and Disease
Author: Roger J. W. Truscott
Publisher: John Wiley & Sons
Total Pages: 224
Release: 2021-04-19
Genre: Science
ISBN: 3527347283

This authoritative overview on an emerging topic in the molecular life sciences covers all aspects of the aging of (long-lived) proteins. It describes the molecular mechanisms of aging on the protein level, in particular the most common side chain modifications and includes analytical methods to study protein half-life and the accumulation of modifications. Finally, the impact of protein aging on several age-related disases in humans is dissected, and their role in limiting human lifespan is discussed.

Lipid Modification of Proteins

Lipid Modification of Proteins
Author: N. M. Hooper
Publisher: Oxford University Press, USA
Total Pages: 318
Release: 1992
Genre: Medical
ISBN:

Lipid Modification of Proteins: A Practical Approach is a unique guide to the latest methods is use, written by the acknowledged experts in the field. Detailed protocols are provided for all the key techniques, and the relevant background material is included. This book is an essential manual for a wide range of scientists studying the modification of protein by lipids, including membrane and protein biochemists, cell biologists, immunologists, bacteriologists, parasitologists, and virologists.

Peptides and Peptide-based Biomaterials and their Biomedical Applications

Peptides and Peptide-based Biomaterials and their Biomedical Applications
Author: Anwar Sunna
Publisher: Springer
Total Pages: 309
Release: 2017-10-26
Genre: Science
ISBN: 3319660950

Solid-binding peptides have been used increasingly as molecular building blocks in nanobiotechnology as they can direct the assembly and functionalisation of a diverse range of materials and have the ability to regulate the synthesis of nanoparticles and complex nanostructures. Nanostructured materials such as β-sheet fibril-forming peptides and α-helical coiled coil systems have displayed many useful properties including stimulus-responsiveness, modularity and multi-functionality, providing potential technological applications in tissue engineering, antimicrobials, drug delivery and nanoscale electronics. The current situation with respect to self-assembling peptides and bioactive matrices for regenerative medicine are reviewed, as well as peptide-target modeling and an examination of future prospects for peptides in these areas.