Molecular Biology Of The Fission Yeast
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Author | : Richard Egel |
Publisher | : Springer Science & Business Media |
Total Pages | : 464 |
Release | : 2013-03-14 |
Genre | : Science |
ISBN | : 3662103605 |
The fission yeast Schizosaccharomyces pombe is the favoured tool of many productive research groups throughout the world, serving as a useful model for fundamental principles and mechanisms, such as genome organization, differential gene regulation, cell-cycle control, signal transduction, or cellular morphogenesis. This book collates the current state of knowledge derived from molecular studies in this simple eukaryotic microorganism. The entire sequence of its genome has been completed, emphasizing the comparative value and model status of this yeast. The individual chapters, highlighting up-to-date views on prominent aspects of molecular organization, were written by active research scientists, presenting the results of their investigations to other workers in neighbouring fields. This book intends to serve the fission yeast community as a handy source of reference for years to come. It will also be of particular value to the ever-increasing number of researchers starting to look into fission yeast affairs for comparative reasons from other platforms of molecular genetics and cell biology.
Author | : |
Publisher | : Elsevier |
Total Pages | : 482 |
Release | : 2012-12-02 |
Genre | : Nature |
ISBN | : 0323140068 |
This highly researched yeast, which represents a system used by cell biologists, geneticists and molecular biologists, has been given only minimal coverage in the literature. Its properties make it an excellent organism for DNA and related biotechnology reseach. This book, which is the first attempt to collate existing information in one source, will be an invaluable aid to those initiating projects with this organism.
Author | : Iain Hagan |
Publisher | : |
Total Pages | : 0 |
Release | : 2016 |
Genre | : Schizosaccharomyces pombe |
ISBN | : 9781621820819 |
Fission yeast are unicellular, rod-shaped fungi that divide by medial fission. Studies using fission yeast were instrumental in identifying fundamental mechanisms that govern cell division, differentiation, and epigenetics, to name but a few. Their rapid growth rate, genetic malleability, and similarities to more complex eukaryotes continue to make them excellent subjects for many biochemical, molecular, and cell biological studies. This laboratory manual provides an authoritative collection of core experimental procedures that underpin modern fission yeast research. The contributors describe basic methods for culturing and genetically manipulating fission yeast, synchronization strategies for probing the cell cycle, technologies for assessing proteins, metabolites, and cell wall constituents, imaging methods to visualize subcellular structures and dynamics, and protocols for investigating chromatin and nucleic acid metabolism. Modifications to techniques commonly used in related species (e.g., budding yeast) are noted, as are useful resources for fission yeast researchers, including various databases and repositories. The well-studied fission yeast Schizosaccharomyces pombe is the focus throughout, but the emerging model S. japonicus-a larger, dimorphic species with several desirable characteristics-is also covered. This manual is an important reference for existing fission yeast laboratories and will serve as an essential start-up guide for those working with fission yeast for the first time.
Author | : Yuko Giga-Hama |
Publisher | : Springer Science & Business Media |
Total Pages | : 239 |
Release | : 2013-03-09 |
Genre | : Science |
ISBN | : 3662034727 |
Recombinant proteins, produced in cells or microorganisms, play an important role in basic research, medical science and the biotechnology industry. Many foreign gene expression systems using mammalian cells, E. coli, insect cells, yeast and other organisms as hosts have been developed. However, only recently has the expression system of Schizosaccharomyces pombe as a host become a subject of focus, as it is expected to be profitable for expressing foreign genes. The reason for this is that S. pombe is evolutionarily more advanced than other types of yeast. This book describes the use of S. pombe in basic research and industrial applications.
Author | : Caroline Alfa |
Publisher | : |
Total Pages | : 186 |
Release | : 1993-01-01 |
Genre | : Science |
ISBN | : 9780879694241 |
A lab manual based on a course at Cold Spring Harbor, in which schizosaccharomyces pombe the fission yeast, is used to investigate the genetic regulation of cell division and other aspects of cell and molecular biology. Presents 21 experiments on cell biology, classical genetics, and molecular genetics. Includes background information on the wee critters and a list of suppliers of equipment and material. No index. Plastic comb binding. Annotation copyright by Book News, Inc., Portland, OR
Author | : Bruce Alberts |
Publisher | : |
Total Pages | : 0 |
Release | : 2002 |
Genre | : Cytology |
ISBN | : 9780815332183 |
Author | : |
Publisher | : Gulf Professional Publishing |
Total Pages | : 961 |
Release | : 2004-05-14 |
Genre | : Science |
ISBN | : 0080575137 |
Guide to Yeast Genetics and Molecular Biology presents, for the first time, a comprehensive compilation of the protocols and procedures that have made Saccharomyces cerevisiae such a facile system for all researchers in molecular and cell biology. Whether you are an established yeast biologist or a newcomer to the field, this volume contains all the up-to-date methods you will need to study "Your Favorite Gene" in yeast. Key Features * Basic Methods in Yeast Genetics * Physical and genetic mapping * Making and recovering mutants * Cloning and Recombinant DNA Methods * High-efficiency transformation * Preparation of yeast artificial chromosome vectors * Basic Methods of Cell Biology * Immunomicroscopy * Protein targeting assays * Biochemistry of Gene Expression * Vectors for regulated expression * Isolation of labeled and unlabeled DNA, RNA, and protein
Author | : Michael N. Hall |
Publisher | : |
Total Pages | : 0 |
Release | : 2008 |
Genre | : |
ISBN | : 9780879698744 |
Yeast genetics began with Winge's 1935 studies of S. cerevisiae in Copenhagen, and afterwards was pursued by Lindegren in the U.S. and Ephrussi in France. Genetic studies in S. pombe were pioneered by Leupold in the 1940s in Switzerland. Within four decades, not without controversies, both yeast species were recognized as essential models in eukaryotic molecular cell biology. In this remarkable volume, Hall and Linder have assembled the reminiscences of many early investigators whose pioneering studies in the years before 1975 brought yeast biology to its current maturity. These illustrated essays about the science, the events and the personalities involved capture a fascinating era, in the informal style made famous by Phage and the Origins of Molecular Biology. This is a book that all scientists interested in the development of modern genetics and molecular biology should have on their shelves.
Author | : Christine Guthrie |
Publisher | : |
Total Pages | : 623 |
Release | : 1991 |
Genre | : Molecular biology |
ISBN | : 9780121820954 |
Author | : Richard Egel |
Publisher | : |
Total Pages | : 480 |
Release | : 2014-01-15 |
Genre | : |
ISBN | : 9783662103616 |