Methods Of Protein And Nucleic Acid Research
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| Author | : Jay A. Glasel |
| Publisher | : Academic Press |
| Total Pages | : 528 |
| Release | : 1995-11-20 |
| Genre | : Science |
| ISBN | : 0080534988 |
The first of its kind, Introduction to Biophysical Methods for Protein and Nucleic Acid Research serves as a text for the experienced researcher and student requiring an introduction to the field. Each chapter presents a description of the physical basis of the method, the type of information that may be obtained with the method, how data should be analyzed and interpreted and, where appropriate, practical tips about procedures and equipment.Key Features* Modern Use of Mass Spectroscopy* NMR Spectroscopy* Molecular Modeling and Graphics* Macintosh and DOS/Windows 3.x disks
| Author | : L.A. Osterman |
| Publisher | : Springer |
| Total Pages | : 368 |
| Release | : 1984-02-01 |
| Genre | : Science |
| ISBN | : 9783540127352 |
Electrophoresis is the leading method among those used in the investigation of pro teins and nucleic acids. A paper on the study of these biopolymers without recourse to electrophoresis at each fractionation or characterization step is very unlikely to be encountered in the current scientific literature. This method enables separation of macromolecules according to characteristic features such as size (or molecular weight), shape, secondary structure and electric charge and these parameters can in fluence electrophoretic properties either separately or jointly. The physical background of this method is as follows. Macromolecules in a buf fer solution may become charged; the sign and magnitude of the total electrical charge depending on the pH of the medium. When such a solution is put into an insulated channel, e.g. in a glass tube, and a voltage applied at the ends of the chan nel, an electric field will be formed and direct current will pass through solution. The field strength can be defined as the potential difference at the ends of the chan nel (or its section) with respect to its length (v/cm). When exposed to an electrical field macromolecules will migrate towards the cathode or the anode according to their net charge and frictional force will limit the migration velocity.
| Author | : Kazuhiko Nakatani |
| Publisher | : Springer |
| Total Pages | : 280 |
| Release | : 2016-04-04 |
| Genre | : Science |
| ISBN | : 3319271113 |
This book spans diverse aspects of modified nucleic acids, from chemical synthesis and spectroscopy to in vivo applications, and highlights studies on chemical modifications of the backbone and nucleobases. Topics discussed include fluorescent pyrimidine and purine analogs, enzymatic approaches to the preparation of modified nucleic acids, emission and electron paramagnetic resonance (EPR) spectroscopy for studying nucleic acid structure and dynamics, non-covalent binding of low- and high-MW ligands to nucleic acids and the design of unnatural base pairs. This unique book addresses new developments and is designed for graduate level and professional research purposes.
| Author | : |
| Publisher | : |
| Total Pages | : 0 |
| Release | : 2002 |
| Genre | : Cells |
| ISBN | : 9780815332183 |
| Author | : Wolfram Saenger |
| Publisher | : |
| Total Pages | : 230 |
| Release | : 1989 |
| Genre | : Science |
| ISBN | : |
This volume contains a series of essays which describe a range of problems in the field of nucleic-acid interactions, investigated by a variety of techniques. An introductory chapter on DNA-protein interactions in the regulation of gene expression is followed by papers on selected model systems.
| Author | : Richard Durbin |
| Publisher | : Cambridge University Press |
| Total Pages | : 372 |
| Release | : 1998-04-23 |
| Genre | : Science |
| ISBN | : 113945739X |
Probabilistic models are becoming increasingly important in analysing the huge amount of data being produced by large-scale DNA-sequencing efforts such as the Human Genome Project. For example, hidden Markov models are used for analysing biological sequences, linguistic-grammar-based probabilistic models for identifying RNA secondary structure, and probabilistic evolutionary models for inferring phylogenies of sequences from different organisms. This book gives a unified, up-to-date and self-contained account, with a Bayesian slant, of such methods, and more generally to probabilistic methods of sequence analysis. Written by an interdisciplinary team of authors, it aims to be accessible to molecular biologists, computer scientists, and mathematicians with no formal knowledge of the other fields, and at the same time present the state-of-the-art in this new and highly important field.
| Author | : National Research Council |
| Publisher | : National Academies Press |
| Total Pages | : 186 |
| Release | : 1987-02-01 |
| Genre | : Computers |
| ISBN | : 0309062284 |
In much of biology, the search for understanding the relation between structure and function is now taking place at the macromolecular level. Proteins, nucleic acids, and polysaccharides are macromolecule--polymers formed from families of simpler subunits. Because of their size and complexity, the polymers are capable of both inter- and intramolecular interactions. These interactions confer upon the polymers distinctive three-dimensional shapes. These tertiary configurations, in turn, determine the function of the macromolecule. Computers have become so inextricably involved in empirical studies of three-dimensional macromolecular structure that mathematical modeling, or theory, and experimental approaches are interrelated aspects of a single enterprise.
| Author | : Jenny Gu |
| Publisher | : John Wiley & Sons |
| Total Pages | : 1105 |
| Release | : 2011-09-20 |
| Genre | : Science |
| ISBN | : 1118210565 |
Structural Bioinformatics was the first major effort to show the application of the principles and basic knowledge of the larger field of bioinformatics to questions focusing on macromolecular structure, such as the prediction of protein structure and how proteins carry out cellular functions, and how the application of bioinformatics to these life science issues can improve healthcare by accelerating drug discovery and development. Designed primarily as a reference, the first edition nevertheless saw widespread use as a textbook in graduate and undergraduate university courses dealing with the theories and associated algorithms, resources, and tools used in the analysis, prediction, and theoretical underpinnings of DNA, RNA, and proteins. This new edition contains not only thorough updates of the advances in structural bioinformatics since publication of the first edition, but also features eleven new chapters dealing with frontier areas of high scientific impact, including: sampling and search techniques; use of mass spectrometry; genome functional annotation; and much more. Offering detailed coverage for practitioners while remaining accessible to the novice, Structural Bioinformatics, Second Edition is a valuable resource and an excellent textbook for a range of readers in the bioinformatics and advanced biology fields. Praise for the previous edition: "This book is a gold mine of fundamental and practical information in an area not previously well represented in book form." —Biochemistry and Molecular Education "... destined to become a classic reference work for workers at all levels in structural bioinformatics...recommended with great enthusiasm for educators, researchers, and graduate students." —BAMBED "...a useful and timely summary of a rapidly expanding field." —Nature Structural Biology "...a terrific job in this timely creation of a compilation of articles that appropriately addresses this issue." —Briefings in Bioinformatics
| Author | : Timir Tripathi |
| Publisher | : Academic Press |
| Total Pages | : 716 |
| Release | : 2022-01-14 |
| Genre | : Science |
| ISBN | : 0323902650 |
Advances in Protein Molecular and Structural Biology Methods offers a complete overview of the latest tools and methods applicable to the study of proteins at the molecular and structural level. The book begins with sections exploring tools to optimize recombinant protein expression and biophysical techniques such as fluorescence spectroscopy, NMR, mass spectrometry, cryo-electron microscopy, and X-ray crystallography. It then moves towards computational approaches, considering structural bioinformatics, molecular dynamics simulations, and deep machine learning technologies. The book also covers methods applied to intrinsically disordered proteins (IDPs)followed by chapters on protein interaction networks, protein function, and protein design and engineering. It provides researchers with an extensive toolkit of methods and techniques to draw from when conducting their own experimental work, taking them from foundational concepts to practical application. - Presents a thorough overview of the latest and emerging methods and technologies for protein study - Explores biophysical techniques, including nuclear magnetic resonance, X-ray crystallography, and cryo-electron microscopy - Includes computational and machine learning methods - Features a section dedicated to tools and techniques specific to studying intrinsically disordered proteins
| Author | : J. Andrew McCammon |
| Publisher | : Cambridge University Press |
| Total Pages | : 256 |
| Release | : 1988-04-29 |
| Genre | : Science |
| ISBN | : 9780521356527 |
This book is a self-contained introduction to the theory of atomic motion in proteins and nucleic acids. An understanding of such motion is essential because it plays a crucially important role in biological activity. The authors, both of whom are well known for their work in this field, describe in detail the major theoretical methods that are likely to be useful in the computer-aided design of drugs, enzymes and other molecules. A variety of theoretical and experimental studies is described and these are critically analyzed to provide a comprehensive picture of dynamic aspects of biomolecular structure and function. The book will be of interest to graduate students and research workers in structural biochemistry (X-ray diffraction and NMR), theoretical chemistry (liquids and polymers), biophysics, enzymology, molecular biology, pharmaceutical chemistry, genetic engineering and biotechnology.
