Methods In Protein Structure And Stability Analysis
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Author | : Vladimir N. Uversky |
Publisher | : Nova Publishers |
Total Pages | : 414 |
Release | : 2007 |
Genre | : Science |
ISBN | : 9781600217043 |
Protein research is a frontier field in science. Proteins are widely distributed in plants and animals and are the principal constituents of the protoplasm of all cells, and consist essentially of combinations of a-amino acids in peptide linkages. Twenty different amino acids are commonly found in proteins, and serve as enzymes, structural elements, hormones, immunoglobulins, etc., and are involved throughout the body, and in photosynthesis. This book gathers new leading-edge research from throughout the world in this exciting and exploding field of research.
Author | : Kenneth P. Murphy |
Publisher | : Springer Science & Business Media |
Total Pages | : 258 |
Release | : 2008-02-04 |
Genre | : Science |
ISBN | : 1592591930 |
In Protein Structure, Stability, and Folding, Kenneth P. Murphy and a panel of internationally recognized investigators describe some of the newest experimental and theoretical methods for investigating these critical events and processes. Among the techniques discussed are the many methods for calculating many of protein stability and dynamics from knowledge of the structure, and for performing molecular dynamics simulations of protein unfolding. New experimental approaches presented include the use of co-solvents, novel applications of hydrogen exchange techniques, temperature-jump methods for looking at folding events, and new strategies for mutagenesis experiments. Unique in its powerful combination of theory and practice, Protein Structure, Stability, and Folding offers protein and biophysical chemists the means to gain a more comprehensive understanding of some of this complex area by detailing many of the major techniques in use today.
Author | : Vladimir N. Uversky |
Publisher | : Nova Publishers |
Total Pages | : 382 |
Release | : 2007 |
Genre | : Circular dichroism |
ISBN | : 9781600214042 |
Author | : Vladimir N. Uversky |
Publisher | : Nova Publishers |
Total Pages | : 326 |
Release | : 2007 |
Genre | : Science |
ISBN | : 9781600217036 |
Protein research is a frontier field in science. Proteins are widely distributed in plants and animals and are the principal constituents of the protoplasm of all cells, and consist essentially of combinations of a-amino acids in peptide linkages. Twenty different amino acids are commonly found in proteins, and serve as enzymes, structural elements, hormones, immunoglobulins, etc., and are involved throughout the body, and in photosynthesis. This book gathers new leading-edge research from throughout the world in this exciting and exploding field of research.
Author | : Bret A. Shirley |
Publisher | : |
Total Pages | : 377 |
Release | : 1995 |
Genre | : Science |
ISBN | : 9781592595273 |
In Protein Stability and Folding: Theory and Practice, world-class scientists present in a single volume a comprehensive selection of hands-on recipes for all of the major techniques needed to understand the conformational stability of proteins, as well as their three-dimensional folding. The distinguished contributors provide clear, step-by-step instructions along with many troubleshooting tips, alternative procedures, and informative explanations about why certain steps are necessary. Even highly skilled researchers will find many time-saving methods. Among the techniques discussed are fluorescent, ultraviolet, and infrared spectroscopy; HPLC peptide mapping; differential scanning calorimetry; and hydrogen exchange. Shirley's Protein Stability and Folding: Theory and Practice will ensure a significant difference in the outcome of your experiments, producing the result desired even for beginners.
Author | : Villafranc |
Publisher | : Academic Press |
Total Pages | : 635 |
Release | : 1990-04-28 |
Genre | : Science |
ISBN | : 0323137768 |
Current Research in Protein Chemistry: Techniques, Structure, and Function reviews new techniques and methods for determining the structure and function of proteins. Topics covered include protein folding and stability, chimeric proteins, amino acid and peptide analysis, mass spectrometric methods, and protein sequencing techniques. This book is divided into six sections comprised of 55 chapters. The discussion begins with a description of microwave irradiation that uses Teflon-Pyrex tubes for protein hydrolysis, followed by the application of high performance capillary electrophoresis to the analysis of amino acids. The sections that follow explore mass spectrometry, protein sequencing, and capillary electrophoresis as well as protein stability, chimeric proteins and enzyme modifications, and protein structure prediction. Chapters focus on the crystal structure of human interleukin-1a, the acid-denatured states of proteins, solubility of recombinant proteins expressed in Escherichia coli, and catalysis by chimeric proteins. This book will be of value to students and researchers interested in protein chemistry.
Author | : Robert A. Copeland |
Publisher | : Springer Science & Business Media |
Total Pages | : 238 |
Release | : 2013-11-11 |
Genre | : Science |
ISBN | : 1475715056 |
As protein science continues to become an increasingly important aspect of academic and commercial sciences and technology, the need has arisen for a ready source of laboratory protocols for the analysis and evaluation of these biological polymers. Methods for Protein Analysis presents the methods most relevant to the generalist bench scientist working with proteins. A concise yet thorough summary, it covers laboratory methods that can be reasonably performed in a standard protein laboratory, without specialized equipment or expertise. Taking a how to approach, this book examines the techniques used to answer common protein analytical questions and describes methods useful in daily laboratory work. Methods for Protein Analysis is the ideal reference for protein laboratories in academic, government and industrial settings. It is an essential benchtop manual for first-year graduate students beginning their laboratory experience as well as for chemists, biochemists, and molecular biologists in the pharmaceutical, biotechnological, food and specialty chemical industries, and for analysts concerned with the purity and structural integrity of protein. Featuring illustrations and a convenient spiral binding, this guide offers a glossary of common abbreviations and a list of suppliers for protein science.
Author | : Eshel Faraggi |
Publisher | : BoD – Books on Demand |
Total Pages | : 412 |
Release | : 2012-04-20 |
Genre | : Science |
ISBN | : 9535105558 |
Since the dawn of recorded history, and probably even before, men and women have been grasping at the mechanisms by which they themselves exist. Only relatively recently, did this grasp yield anything of substance, and only within the last several decades did the proteins play a pivotal role in this existence. In this expose on the topic of protein structure some of the current issues in this scientific field are discussed. The aim is that a non-expert can gain some appreciation for the intricacies involved, and in the current state of affairs. The expert meanwhile, we hope, can gain a deeper understanding of the topic.
Author | : |
Publisher | : |
Total Pages | : 0 |
Release | : 2002 |
Genre | : Cells |
ISBN | : 9780815332183 |
Author | : David S. Eisenberg |
Publisher | : |
Total Pages | : 334 |
Release | : 1995 |
Genre | : Science |
ISBN | : 9780120342464 |
The topics covered by this volume include: protein destabilization at low temperatures; engineering the stability and function of Gene V Protein; free energy balance in protein folding; modelling protein stability as a heteropolymer collapse; stability of alpha helices; protein stability with T4 Lysozyme.