Methods In Molecular Biology Mycoplasma Protocols
Download Methods In Molecular Biology Mycoplasma Protocols full books in PDF, epub, and Kindle. Read online free Methods In Molecular Biology Mycoplasma Protocols ebook anywhere anytime directly on your device. Fast Download speed and no annoying ads. We cannot guarantee that every ebooks is available!
| Author | : John M. Walker |
| Publisher | : |
| Total Pages | : |
| Release | : 1984 |
| Genre | : Molecular biology -- v.236. Plant functional genomics -- v.237. G protein signaling -- v.238. Biopolymer methods in tissue engineering -- v.239. Cell migration in inflammation and immunity -- v.240. Mammalian artificial chromosomes -- v.241. Cell cycle checkpoint control protocols -- v.242. Atomic force microscopy -- v.243. Chiral separations: Methods and protocols -- v.244. Protein purification protocols -- v.245-6. Gene delivery to mammalian cells -- v.247. Drosophila cytogenetics protocols -- v.248. Antibody engineering -- v.249. Cytokine protocols -- v.251. HPLC of peptides and proteins: Methods and protocols -- v.265. RNA interference, editing, and modification -- v.274. Photosynthesis research protocols -- v.318. Plant cell culture protocols -- v.323. Arabidopsis protocols |
| ISBN | : |
| Author | : Roger J. Miles |
| Publisher | : |
| Total Pages | : 330 |
| Release | : 1998 |
| Genre | : Medical |
| ISBN | : 9781592592692 |
In Mycoplasma Protocols, Roger Miles and Robin Nicholas present a collection of cutting-edge methods for the detection, isolation, identification, characterization, and genetic manipulation of the pathogenic mycoplasmas. These step-by-step methods are crafted for successful reproducibility and include biochemical, genetic, and molecular techniques essential to understanding pathogenicity and adhesion to host cells. They also cover the detection of mycoplasmas in cell cultures, an important tool not only in viral diagnosis and research, but also in the production of vaccines and various biological products. Mycoplasma Protocols provides up-to-date and easy-to-follow mycoplasma methods for practical application in medical and veterinary diagnostic and research laboratories. The techniques permit effective work with these normally fastidious microorganisms, allowing investigators to illuminate their roles across a wide range of chronic respiratory, arthritic, and urogenital diseases. For all those working in this important field, Mycoplasma Protocols immediately becomes the methodological resource of choice.
| Author | : Roger J. Miles |
| Publisher | : Springer Science & Business Media |
| Total Pages | : 362 |
| Release | : 1998-05-25 |
| Genre | : Science |
| ISBN | : |
This is a collection of cutting-edge mycoplasma methods for the detection, isolation, identification, characterization, and genetic manipulation of the pathogenic mycoplasmas. These step-by-step methods are crafted for successful reproducibility and include biochemical, genetic, and molecular techniques essential to understanding pathogenecity and adhesion to host cells. They also cover the detection of mycoplasmas in cell cultures, an important tool not only in viral diagnosis and research, but also in the production of vaccines and various biological products.
| Author | : Cheryl D. Helgason |
| Publisher | : Humana Press |
| Total Pages | : 550 |
| Release | : 2012-11-20 |
| Genre | : Science |
| ISBN | : 9781627031271 |
At some point in their careers, virtually every scientist and technician, as well as many medical professionals, regardless of their area of specialization have a need to utilize cell culture systems. Updating and significantly expanding upon the previous editions, Basic Cell Culture Protocols, Fourth Edition provides the novice cell culturist with sufficient information to perform the basic techniques, to ensure the health and identity of their cell lines, and to be able to isolate and culture specialized primary cell types. The intent of this extensive volume is to generate a valuable resource containing clear methodologies pertinent to current areas of investigation, rather than attempting to educate cell culturists on specific cell types or organ systems. Written in the highly successful Methods in Molecular BiologyTM, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and up-to-date, Basic Cell Culture Protocols, Fourth Edition compiles the essential techniques needed to approach this vital laboratory activity with full success.
| Author | : |
| Publisher | : Academic Press |
| Total Pages | : 489 |
| Release | : 1996-02-21 |
| Genre | : Science |
| ISBN | : 0080536794 |
This book and its companion, Volume I, concentrate on new procedures--especially those based on the new molecular methodology--developed within the past decade. This volume deals with the new genetic and immunological tools applied to the diagnosis of mycoplasma infections of humans, animals, plants, insects, and all cultures. Volume I outlines the approaches, techniques, and procedures applied to cell and molecular biology studies of mycoplasmas. Diagnostic genetic probes Immunological tools Antibiotic sensitivity testing Diagnosis of specific diseases Experimental infections Diagnosis of mycoplasma infections of cell cultures
| Author | : Cheryl D. Helgason |
| Publisher | : Humana Press |
| Total Pages | : 371 |
| Release | : 2014-11-27 |
| Genre | : Science |
| ISBN | : 9781627039307 |
In this fully revised edition of an established classic, expert researchers and clinicians describe in step-by-step detail updated techniques for the isolation and growth of major primary cell types, such as kidney proximal tubule cells, hepatocytes, keratinocytes, and cardiomyocytes. The authors offer readily reproducible new methods for the differentiation of embryonic stem (ES) cells into various hematopoietic cell types, for fetal thymic organ culture, and for the isolation and culture of specialized cell types, such as mammary progenitor cells, skeletal muscle myofibers, mesenchymal cells, neural stem cells, hematopoietic cells, stromal cell lines, and endothelial cells. Additional chapters describe new techniques (leukocyte rolling, isolation of side-population cells, and scalable production of ES-derived cells) and detail quality control methods for cell lines (detection and elimination of mycoplasma, DNA fingerprinting, and cytogenetic analysis).
| Author | : Frederick M. Ausubel |
| Publisher | : |
| Total Pages | : 852 |
| Release | : 1992 |
| Genre | : Biología molecular |
| ISBN | : |
| Author | : Sandeep Singh |
| Publisher | : Bentham Science Publishers |
| Total Pages | : 186 |
| Release | : 2020-01-23 |
| Genre | : Science |
| ISBN | : 981143929X |
Protocols used in Molecular Biology is a compilation of several examples of molecular biology protocols. Each example is presented with a concise introduction, materials and chemicals required, a step-by-step procedure and troubleshooting tips. Information about the application of the protocol is also provided. The techniques included in this book are essential to research in the fields of proteomics, genomics, cell culture, epigenetic modification and structural biology. The protocols can also be used by clinical researchers (neuroscientists and oncologists, for example) for medical applications (diagnostics, therapeutics and multidisciplinary projects).
| Author | : Shmuel Razin |
| Publisher | : Springer Science & Business Media |
| Total Pages | : 592 |
| Release | : 2002-06-30 |
| Genre | : Medical |
| ISBN | : 9780306472879 |
This text is a comprehensive, multi-authored treatise intended for mycoplasmologists as well as molecular biologists, geneticists and bioinformatics researchers newly attracted to mycoplasmas as research models.
| Author | : Frederick M. Ausubel |
| Publisher | : Current Protocols |
| Total Pages | : 1134 |
| Release | : 1999-05-03 |
| Genre | : Medical |
| ISBN | : |
Short Protocols in Molecular Biology Fourth Edition The Desktop Guide to Your Lab Edited by Frederick M. Ausubel, Roger Brent, Robert E. Kingston, David D. Moore, J. G. Seidman, John A. Smith, and Kevin Struhl Providing condensed descriptions of more than 600 methods compiled from Current Protocols in Molecular Biology, this updated edition of the classic laboratory manual thoroughly explores molecular biology in an easily accessible, hands-on format. Examining the physiochemical organization of living matter from a molecular basis requires a text which is informative and well annotated-Short Protocols in Molecular Biology, Fourth Edition offers both. The book is specifically designed to provide quick access to step-by-step instructions for the essential methods used in every major area of molecular biological research. The authors have enriched the text with diagrams, charts, and material lists to enhance comprehension of the material and facilitate the experimental set-up. This edition has been expanded to include the latest developments in cutting-edge techniques such as fluorescent DNA sequencing, PCR optimization, yeast two-hybrid/interaction trap analysis, and sequence similarity searching using Blast. Classic techniques in plasmid and phage manipulation and mammalian cell selection have also benefited from the updating and reflect the methods currently used in leading research facilities around the world. New topics to this edition include: * Informatics for Molecular Biologists * Analysis of Protein Interactions * Epitope Tagging * Mathematics and Statistics for Molecular Biologists Short Protocols in Molecular Biology, Fourth Edition is an authoritative and indispensable guide for all life scientists and researchers who are looking to improve their understanding of molecular biology methods.
