This practical guidance presents details on how to use the EX-Ante Carbon-balance Tool for Value Chain (EX-ACT VC), with the aim of providing a multi-impact appraisal in terms of socio-economic and environmental assessment for value chain analysis. Following the EX-ACT logic, EX-ACT VC compares the environmental and socio-economic performances of current value chains with those of an improved scenario (applicable to different degrees of the chain), based on the analysis of agricultural production, land use changes, processing, transport and climate resilience. EX-ACT VC has been developed for all types of agricultural sectors (which include here fisheries and livestock) that have the potential to mitigate the effects of climate change and to develop the resilience of rural populations that are dependent on them. The tool targets value chains in developing countries for single or more complex sectors, taking into account several agricultural practices and one end-type specific to a region or area.