Quantitative ultrasound (QUS) continues to mature as a research field and is primed to make a swift transition to routine preclinical and clinical applications. This book will serve two main purposes: Advanced education in QUS by providing a complete and thorough review of all theoretical, physical, and engineering aspects of QUS. Review of recent development of QUS by lead contributors in the research field. This 2nd edition will focus on 6 modern research topics related to quantitative ultrasound of soft tissues: Spectral-based methods for tissue characterization, tissue typing, cancer detection, etc. Attenuation estimation for tissue characterization and improving spectral based methods Envelope statistics analysis as a means of quantifying and imaging tissue properties. Ultrasound computed tomography for preclinical and clinical imaging. Scanning acoustic microscopy for forming images of mechanical properties of soft tissues with micron resolution. Phantoms for quantitative ultrasound.