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| Author | : S. Rajan |
| Publisher | : CBS Publishers & Distributors Pvt Limited, India |
| Total Pages | : |
| Release | : 2018-04-30 |
| Genre | : Medical |
| ISBN | : 9789386478252 |
This is a manual for all life science students studying courses in biochemistry, biotechnology, botany, genetics, microbiology, molecular biology, zoology, nursing, and medicine, based on the author's decades-long experience in the field experiments of life sciences teaching and research.
| Author | : Graeme D. Ruxton |
| Publisher | : |
| Total Pages | : 0 |
| Release | : 2023 |
| Genre | : Experimental design |
| ISBN | : 9780191975202 |
Providing students with clear and practical advice on how best to organise experiments and collect data so as to make the subsequent analysis easier and their conclusions more robust, this text assumes no specialist knowledge.
| Author | : Morteza Jalali |
| Publisher | : Academic Press |
| Total Pages | : 383 |
| Release | : 2017-03-31 |
| Genre | : Science |
| ISBN | : 012803078X |
Basic Science Methods for Clinical Researchers addresses the specific challenges faced by clinicians without a conventional science background. The aim of the book is to introduce the reader to core experimental methods commonly used to answer questions in basic science research and to outline their relative strengths and limitations in generating conclusive data. This book will be a vital companion for clinicians undertaking laboratory-based science. It will support clinicians in the pursuit of their academic interests and in making an original contribution to their chosen field. In doing so, it will facilitate the development of tomorrow's clinician scientists and future leaders in discovery science. - Serves as a helpful guide for clinical researchers who lack a conventional science background - Organized around research themes pertaining to key biological molecules, from genes, to proteins, cells, and model organisms - Features protocols, techniques for troubleshooting common problems, and an explanation of the advantages and limitations of a technique in generating conclusive data - Appendices provide resources for practical research methodology, including legal frameworks for using stem cells and animals in the laboratory, ethical considerations, and good laboratory practice (GLP)
| Author | : Zachary F. Burton |
| Publisher | : Academic Press |
| Total Pages | : 252 |
| Release | : 1997-02-20 |
| Genre | : Science |
| ISBN | : 9780121473709 |
Experiments in Molecular Biology provides a thorough introduction to recombinant DNA methods used in molecular biology and nucleic acid biochemistry. This unique laboratory manual is particularly appropriate for courses in molecular cloning, molecular genetics techniques, molecular biology techniques, recombinant DNA techniques, bacterial genetics techniques, and genetic engineering. Included is an especially helpful section to aid new instructors in avoiding potential pitfalls of specific experiments. Key Features * Contains student-tested, easy-to-follow protocols * Presents background information that reinforces principles behind the methods presented * Includes questions at the end of laboratory exercises * Provides both detailed descriptions of experimental procedures and a theoretical support section * Sequentially links experiments to provide a "project" approach to studying molecular biochemistry * Includes student-tested, easy-to-follow protocols * Background information reinforces principles behind the methods presented * Includes questions at the end of laboratory exercises * Advises new instructors on potential pitfalls of specific experiments * Provides both detailed descriptions of experimental procedures and a theoretical support section * Sequentially links experiments to provide a "project" approach to studying
| Author | : National Academies of Sciences, Engineering, and Medicine |
| Publisher | : National Academies Press |
| Total Pages | : 257 |
| Release | : 2019-10-20 |
| Genre | : Science |
| ISBN | : 0309486165 |
One of the pathways by which the scientific community confirms the validity of a new scientific discovery is by repeating the research that produced it. When a scientific effort fails to independently confirm the computations or results of a previous study, some fear that it may be a symptom of a lack of rigor in science, while others argue that such an observed inconsistency can be an important precursor to new discovery. Concerns about reproducibility and replicability have been expressed in both scientific and popular media. As these concerns came to light, Congress requested that the National Academies of Sciences, Engineering, and Medicine conduct a study to assess the extent of issues related to reproducibility and replicability and to offer recommendations for improving rigor and transparency in scientific research. Reproducibility and Replicability in Science defines reproducibility and replicability and examines the factors that may lead to non-reproducibility and non-replicability in research. Unlike the typical expectation of reproducibility between two computations, expectations about replicability are more nuanced, and in some cases a lack of replicability can aid the process of scientific discovery. This report provides recommendations to researchers, academic institutions, journals, and funders on steps they can take to improve reproducibility and replicability in science.
| Author | : Stefan Surzycki |
| Publisher | : Springer Science & Business Media |
| Total Pages | : 442 |
| Release | : 2012-12-06 |
| Genre | : Science |
| ISBN | : 3642569684 |
This laboratory manual gives a thorough introduction to basic techniques. It is the result of practical experience, with each protocol having been used extensively in undergraduate courses or tested in the authors laboratory. In addition to detailed protocols and practical notes, each technique includes an overview of its general importance, the time and expense involved in its application and a description of the theoretical mechanisms of each step. This enables users to design their own modifications or to adapt the method to different systems. Surzycki has been holding undergraduate courses and workshops for many years, during which time he has extensively modified and refined the techniques described here.
| Author | : Prakash Singh Bisen |
| Publisher | : CRC Press |
| Total Pages | : 1772 |
| Release | : 2014-02-26 |
| Genre | : Medical |
| ISBN | : 1466553154 |
As applied life science progresses, becoming fully integrated into the biological, chemical, and engineering sciences, there is a growing need for expanding life sciences research techniques. Anticipating the demands of various life science disciplines, Laboratory Protocols in Applied Life Sciences explores this development. This book covers a wide spectrum of areas in the interdisciplinary fields of life sciences, pharmacy, medical and paramedical sciences, and biotechnology. It examines the principles, concepts, and every aspect of applicable techniques in these areas. Covering elementary concepts to advanced research techniques, the text analyzes data through experimentation and explains the theory behind each exercise. It presents each experiment with an introduction to the topic, concise objectives, and a list of necessary materials and reagents, and introduces step-by-step, readily feasible laboratory protocols. Focusing on the chemical characteristics of enzymes, metabolic processes, product and raw materials, and on the basic mechanisms and analytical techniques involved in life science technological transformations, this text provides information on the biological characteristics of living cells of different origin and the development of new life forms by genetic engineering techniques. It also examines product development using biological systems, including pharmaceutical, food, and beverage industries. Laboratory Protocols in Applied Life Sciences presents a nonmathematical account of the underlying principles of a variety of experimental techniques in disciplines, including: Biotechnology Analytical biochemistry Clinical biochemistry Biophysics Molecular biology Genetic engineering Bioprocess technology Industrial processes Animal Plant Microbial biology Computational biology Biosensors Each chapter is self-contained and written in a style that helps students progress from basic to advanced techniques, and eventually design and execute their own experiments in a given field of biology.
| Author | : National Research Council |
| Publisher | : National Academies Press |
| Total Pages | : 136 |
| Release | : 2007-11-10 |
| Genre | : Political Science |
| ISBN | : 0309111919 |
Based on a series of regional meetings on university campuses with officials from the national security community and academic research institutions, this report identifies specific actions that should be taken to maintain a thriving scientific research environment in an era of heightened security concerns. Actions include maintaining the open exchange of scientific information, fostering a productive environment for international scholars in the U.S., reexamining federal definitions of sensitive but unclassified research, and reviewing policies on deemed export controls. The federal government should establish a standing entity, preferably a Science and Security Commission, that would review policies regarding the exchange of information and the participation of foreign-born scientists and students in research.
| Author | : S. Harisha |
| Publisher | : Laxmi Publications, Ltd. |
| Total Pages | : 726 |
| Release | : 2008-12 |
| Genre | : Science |
| ISBN | : 9788131803790 |
Biotechnology Is One Of The Major New Technologies Of The Twenty-First Century That Covers Multi-Disciplinary Issues, Including Recombinant DNA Techniques, Cloning, Genetics, And The Application Of Microbiology To The Production Of Goods. It Continues To Revolutionize Treatments Of Many Diseases, And It Is Used To Deal With Environmental Solutions. The Biotechnology Procedures And Experiments Handbook Provides Practicing Professionals And Biotechnology Students Over 150 Applied, Up-To-Date Laboratory Techniques And Experiments Related To Modern Topics Such As Recombinant DNA, Electrophoresis, Stem Cell Research, Genetic Engineering, Microbiology, Tissue Culture, And More. Each Lab Technique Includes 1)A Principle, 2)The Necessary Reagents, 3)A Step By Step Procedure, And 4)A Final Result. Also Included Is A Section That Shows How To Avoid Potential Pitfalls Of A Specific Experiment. The Book Is Accompanied By A CD-ROM Containing Simulations, White Papers, And Other Relevant Material To Biotechnology.
| Author | : Frank H. Stephenson |
| Publisher | : Academic Press |
| Total Pages | : 520 |
| Release | : 2010-07-30 |
| Genre | : Science |
| ISBN | : 012375691X |
Calculations for Molecular Biology and Biotechnology: A Guide to Mathematics in the Laboratory, Second Edition, provides an introduction to the myriad of laboratory calculations used in molecular biology and biotechnology. The book begins by discussing the use of scientific notation and metric prefixes, which require the use of exponents and an understanding of significant digits. It explains the mathematics involved in making solutions; the characteristics of cell growth; the multiplicity of infection; and the quantification of nucleic acids. It includes chapters that deal with the mathematics involved in the use of radioisotopes in nucleic acid research; the synthesis of oligonucleotides; the polymerase chain reaction (PCR) method; and the development of recombinant DNA technology. Protein quantification and the assessment of protein activity are also discussed, along with the centrifugation method and applications of PCR in forensics and paternity testing. - Topics range from basic scientific notations to complex subjects like nucleic acid chemistry and recombinant DNA technology - Each chapter includes a brief explanation of the concept and covers necessary definitions, theory and rationale for each type of calculation - Recent applications of the procedures and computations in clinical, academic, industrial and basic research laboratories are cited throughout the text New to this Edition: - Updated and increased coverage of real time PCR and the mathematics used to measure gene expression - More sample problems in every chapter for readers to practice concepts
