Epidural Spinal Stimulation is Associated with Changes in Surviving Axons and Associated Glial Cells After Spinal Cord Injury in Rodents

Epidural Spinal Stimulation is Associated with Changes in Surviving Axons and Associated Glial Cells After Spinal Cord Injury in Rodents
Author: Kyleigh Alexis DePetro
Publisher:
Total Pages: 46
Release: 2017
Genre:
ISBN:

We previously demonstrated that epidural spinal stimulation (ES) and transcutaneous stimulation combined with step training can restore voluntary motor control in humans with chronic, motor-complete spinal cord injuries in at least 24 hours. The purpose of this study was to determine the underlying pathways involved. We hypothesized that chronic ES can provide motor improvements that persist in the absence of stimulation and that both surviving axons and their associated glial cells play important roles in ES-facilitated recovery. Our kinematic data suggested that chronic ES is associated with persistent motor recovery when ES is off. Our histological analysis demonstrated that the node of Ranvier, oligodendrocytes, astrocytic process extension, and apoptosis play important roles in mediating axonal integrity associated with functional motor improvement with ES in rats.

Transplantation of Olfactory Ensheathing Cells Combined with Epidural Stimulation and Climb Training as a Long-term Treatment for Severe Spinal Cord Injury in Rodents

Transplantation of Olfactory Ensheathing Cells Combined with Epidural Stimulation and Climb Training as a Long-term Treatment for Severe Spinal Cord Injury in Rodents
Author: Kaitlin Lee Ingraham Dixie
Publisher:
Total Pages: 186
Release: 2019
Genre:
ISBN:

Following a severe spinal cord injury (SCI), transplanted olfactory ensheathing cells (OECs) reduce inhibitory factors and promote axonal outgrowth at the lesion site to help reestablish circuit connectivity needed for functional recovery. Another promising therapy, electrical epidural stimulation (EES), also helps to reestablish connectivity but targets the spinal cord caudal to the injury. This study asked if the combination of OEC transplantation and administration of EES during climb training would improve recovery. Two cohorts of inbred Fischer 344 rats received a severe SCI and a two-week delayed transplant of OECs, media, or fibroblasts (FBs). Rats then received EES while performing a climbing task 3 times/week and were perfused at 5.5-6.5 months post-injury. We found that scores on the BBB locomotor test improved for all groups over time, but no consistent changes in climbing ability were detected. When we examined the injury sites, we found surviving GFP-labeled OECs and FBs for the first time at 6 months post-transplant. We also found that OEC treatment increased the amount of serotonergic and neurofilament (NF)-positive axons in the lesion core compared to media- and FB-treated controls. This was particularly true in areas of the lesion core with OECs where we found greater levels of axon density than in areas without OECs. Further, the percent of NF-positive axons associated with myelin proteins was greater in OEC- compared to media-treated rats. The OECs in the lesion core also interacted with serotonergic axons, myelinating and non-myelinating Schwann cells (SCs), and oligodendrocytes and expressed protein markers typically associated with SC-like myelin. Overall our results provide evidence that OEC transplantation combined with EES may be a beneficial treatment for severe SCI.

The Spinal Cord

The Spinal Cord
Author: Charles Watson
Publisher: Academic Press
Total Pages: 408
Release: 2009-11-27
Genre: Medical
ISBN: 0080921388

Many hundreds of thousands suffer spinal cord injuries leading to loss of sensation and motor function in the body below the point of injury. Spinal cord research has made some significant strides towards new treatment methods, and is a focus of many laboratories worldwide. In addition, research on the involvement of the spinal cord in pain and the abilities of nervous tissue in the spine to regenerate has increasingly been on the forefront of biomedical research in the past years. The Spinal Cord, a collaboration with the Christopher and Dana Reeve Foundation, is the first comprehensive book on the anatomy of the mammalian spinal cord. Tens of thousands of articles and dozens of books are published on this subject each year, and a great deal of experimental work has been carried out on the rat spinal cord. Despite this, there is no comprehensive and authoritative atlas of the mammalian spinal cord. Almost all of the fine details of spinal cord anatomy must be searched for in journal articles on particular subjects. This book addresses this need by providing both a comprehensive reference on the mammalian spinal cord and a comparative atlas of both rat and mouse spinal cords in one convenient source. The book provides a descriptive survey of the details of mammalian spinal cord anatomy, focusing on the rat with many illustrations from the leading experts in the field and atlases of the rat and the mouse spinal cord. The rat and mouse spinal cord atlas chapters include photographs of Nissl stained transverse sections from each of the spinal cord segments (obtained from a single unfixed spinal cord), detailed diagrams of each of the spinal cord segments pictured, delineating the laminae of Rexed and all other significant neuronal groupings at each level and photographs of additional sections displaying markers such as acetylcholinesterase (AChE), calbindin, calretinin, choline acetlytransferase, neurofilament protein (SMI 32), enkephalin, calcitonin gene-related peptide (CGRP), and neuronal nuclear protein (NeuN). The text provides a detailed account of the anatomy of the mammalian spinal cord and surrounding musculoskeletal elements The major topics addressed are: development of the spinal cord; the gross anatomy of the spinal cord and its meninges; spinal nerves, nerve roots, and dorsal root ganglia; the vertebral column, vertebral joints, and vertebral muscles; blood supply of the spinal cord; cytoarchitecture and chemoarchitecture of the spinal gray matter; musculotopic anatomy of motoneuron groups; tracts connecting the brain and spinal cord; spinospinal pathways; sympathetic and parasympathetic elements in the spinal cord; neuronal groups and pathways that control micturition; the anatomy of spinal cord injury in experimental animals The atlas of the rat and mouse spinal cord has the following features: Photographs of Nissl stained transverse sections from each of 34 spinal segments for the rat and mouse; Detailed diagrams of each of the 34 spinal segments for rat and mouse, delineating the laminae of Rexed and all other significant neuronal groupings at each level. ; Alongside each of the 34 Nissl stained segments, there are additional sections displaying markers such as acetylcholinesterase, calbindin, calretinin, choline acetlytransferase, neurofilament protein (SMI 32), and neuronal nuclear protein (NeuN) All the major motoneuron clusters are identified in relation to the individual muscles or muscle groups they supply

Spinal Cord Injury (SCI) Repair Strategies

Spinal Cord Injury (SCI) Repair Strategies
Author: Giuseppe Perale
Publisher: Woodhead Publishing
Total Pages: 346
Release: 2019-10-30
Genre: Medical
ISBN: 0081028083

Spinal Cord Injury (SCI) Repair Strategies provides researchers the latest information on potential regenerative approaches to spinal cord injury, specifically focusing on therapeutic approaches that target regeneration, including cell therapies, controlled drug delivery systems, and biomaterials. Dr. Giuseppe Perale and Dr. Filippo Rossi lead a team of authoritative authors in academia and industry in this innovative reference on the field of regenerative medicine and tissue engineering. This book presents all the information readers need to understand the current and potential array of techniques, materials, applications and their benefits for spinal cord repair. Covers current and future repair strategies for spinal cord injury repair Focuses on key research trends, clinics, biology and engineering Provides fundamentals on regenerative engineering and tissue engineering

Indwelling Neural Implants

Indwelling Neural Implants
Author: William M. Reichert
Publisher: CRC Press
Total Pages: 300
Release: 2007-12-17
Genre: Medical
ISBN: 1420009303

Despite enormous advances made in the development of external effector prosthetics over the last quarter century, significant questions remain, especially those concerning signal degradation that occurs with chronically implanted neuroelectrodes. Offering contributions from pioneering researchers in neuroprosthetics and tissue repair, Indwel

Neurorestoratology

Neurorestoratology
Author: Hongyun Huang
Publisher: Nova Science Publishers
Total Pages: 0
Release: 2015
Genre: Nervous system
ISBN: 9781634636001

Neurorestoratology is one of the most important disciplines in modern medicine and is also the most important discipline in neuroscience. Its core aim is to restore, promote and maintain the integrity of impaired or lost neuronal functions and/or structures by using novel cell-based comprehensive neurorestorative strategies. This book is the first and a unique one that systematically expounds the main aspects of neurorestoratology, which includes three sections with 22 chapters in two volumes. It systematically elaborates CNS neurorestorable theory and neurorestorative mechanisms. It firstly comprehends the Neurorestorative Process as a whole and Neurorestorative law. It fully describes all neurorestorative strategies and their continuing clinical progresses and achievements, especially the cell-based comprehensive neurorestorative strategies.

Brain Repair After Stroke

Brain Repair After Stroke
Author: Steven C. Cramer
Publisher: Cambridge University Press
Total Pages: 307
Release: 2010-10-28
Genre: Medical
ISBN: 1139490656

Increasing evidence identifies the possibility of restoring function to the damaged brain via exogenous therapies. One major target for these advances is stroke, where most patients can be left with significant disability. Treatments have the potential to improve the victim's quality of life significantly and reduce the time and expense of rehabilitation. Brain Repair After Stroke reviews the biology of spontaneous brain repair after stroke in animal models and in humans. Detailed chapters cover the many forms of therapy being explored to promote brain repair and consider clinical trial issues in this context. This book provides a summary of the neurobiology of innate and treatment-induced repair mechanisms after hypoxia and reviews the state of the art for human therapeutics in relation to promoting behavioral recovery after stroke. Essential reading for stroke physicians, neurologists, rehabilitation physicians and neuropsychologists.

Translational Research in Traumatic Brain Injury

Translational Research in Traumatic Brain Injury
Author: Daniel Laskowitz
Publisher: CRC Press
Total Pages: 388
Release: 2016-04-21
Genre: Medical
ISBN: 1498766579

Traumatic brain injury (TBI) remains a significant source of death and permanent disability, contributing to nearly one-third of all injury related deaths in the United States and exacting a profound personal and economic toll. Despite the increased resources that have recently been brought to bear to improve our understanding of TBI, the developme

Axonal Regeneration is Detected After Olfactory Ensheathing Cell Or Fibroblast Transplantation in Sprague-Dawley Rats with Completely Transected Spinal Cords

Axonal Regeneration is Detected After Olfactory Ensheathing Cell Or Fibroblast Transplantation in Sprague-Dawley Rats with Completely Transected Spinal Cords
Author: Michael Andrew Thornton
Publisher:
Total Pages: 68
Release: 2016
Genre:
ISBN:

Olfactory ensheathing cells (OECs) are unique glia that support axon outgrowth in the olfactory system and have shown some success as a cellular transplant therapy for the recovery of sensorimotor control after spinal cord injury. A pilot study was designed in which 10 female Sprague-Dawley rats received acute transplantation of skin fibroblasts (FB, control, n=5) or OECs (n=5) after a complete mid-thoracic spinal cord transection. All rats were implanted with epidural stimulating electrodes at spinal cord levels L1 and S2 and trained to climb an inclined grid while receiving sub-threshold stimulation for 20 min., 3 times/week for 6 months. We injected the Bartha-152 (EGFP-expressing) strain of pseudorabies virus (PRV) into the soleus and/or tibialis anterior muscles 6 days before termination to identify hindlimb motor circuits and assess connectivity across the injury site. Viral transport to cholinergic somatic motor neurons and premotor interneurons was detected in 8 rats (4 FB, 4 OEC). Three rats (2 FB, 1 OEC) had evidence of viral labeling rostral to the transection site (T3-6), including cholinergic and Chx10-positive V2a interneurons. Serotonergic axons crossed from the rostral to the caudal stump on GFAP-positive astrocyte bridges in 2 of the 3 rats with evidence of PRVeGFP labeling above the injury site (1 FB, 1 OEC). Together these data imply that long-term axonal regeneration occurred in two of our complete spinal rats after epidural stimulation, climb training, and olfactory ensheathing cell or fibroblast transplantation.

Galanin

Galanin
Author: Tomas Hökfelt
Publisher: Springer Science & Business Media
Total Pages: 285
Release: 2010-08-18
Genre: Science
ISBN: 3034602286

Galanin is a neuropeptide found both in the central and peripheral nervous system. The 29-amino acid peptide (named after its N-terminal glycine and C-terminal alanine) was identified in 1983 by its C-terminal amidation. This 'reverse' approach, that is to discover a substance through a distinct chemical feature, and only subsequently to characterize its biological activity, was novel and has been successful in the identification of several other peptides. After the structure of galanin was determined in 1983, functional studies were performed with material purified from natural sources until the synthetic form of the peptide became available. Galanin can act as transmitter, modulator and trophic factor, and is involved in a number of physiological processes such as hormone secretion, cardiovascular mechanisms, feeding and cognition. This peptide may also be of significance for a number of pathological processes/disorders including pain, depression, Alzheimer's disease, epilepsy, addiction and cancer. This wide diversity of actions is mediated by three galanin receptor subtypes. The studies reviewed in this volume give a fairly complete overview of the spectrum of the biological actions and functions of galanin and its receptors and on possible therapeutic applications in a number of pathological conditions.