Enzyme Engineering And Evolution Specific Enzyme Applications
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Author | : |
Publisher | : Academic Press |
Total Pages | : 312 |
Release | : 2020-09-15 |
Genre | : Science |
ISBN | : 0128244321 |
Methods in Enzymology, Volume 644, the latest release in this ongoing serial, continues the legacy of this premier serial with quality chapters authored by leaders in the field. Chapters in this new release include Site-directed recombination (SDR) in vivo: a fast and reliable tool to unveil beneficial epistasis, Creation and application of amine oxidase with expanded substrate specificities from porcine kidney D-amino acid oxidase, Methods to assess correlation networks for engineering transketolase, Exploration of Enzyme Diversity by Integrating Bioinformatics with Microfluidics, Engineering lytic polysaccharide monooxygenases (LPMOs), Emulsion-based directed evolution of enzymes in yeast, and much more. Provides the authority and expertise of leading contributors from an international board of authors Presents the latest release in the Methods in Enzymology series
Author | : |
Publisher | : Academic Press |
Total Pages | : 362 |
Release | : 2020-09-05 |
Genre | : Science |
ISBN | : 0128211504 |
This new volume of Methods in Enzymology continues the legacy of this premier serial with quality chapters authored by leaders in the field. Provides the authority and expertise of leading contributors from an international board of authors Presents the latest release in the Methods in Enzymology series
Author | : Miguel Alcalde |
Publisher | : Springer |
Total Pages | : 286 |
Release | : 2017-02-14 |
Genre | : Science |
ISBN | : 3319504134 |
This book focuses on some of the most significant advances in enzyme engineering that have been achieved through directed evolution and hybrid approaches. On the 25th anniversary of the discovery of directed evolution, this volume is a tribute to the pioneers of this thrilling research field, and at the same time provides a comprehensive overview of current research and the state of the art. Directed molecular evolution has become the most reliable and robust method to tailor enzymes, metabolic pathways or even whole microorganisms with improved traits. By mirroring the Darwinian algorithm of natural selection on a laboratory scale, new biomolecules of invaluable biotechnological interest can now be engineered in a manner that surpasses the boundaries of nature. The volume is divided into two sections, the first of which provides an update on recent successful cases of enzyme ensembles from different areas of the biotechnological spectrum, including tryptophan synthases, unspecific peroxygenases, phytases, therapeutic enzymes, stereoselective enzymes and CO2-fixing enzymes. This section also provides information on the directed evolution of whole cells. The second section of the book summarizes a variety of the most applicable methods for library creation, together with the future trends aimed at bringing together directed evolution and in silico/computational enzyme design and ancestral resurrection.
Author | : Frances H. Arnold |
Publisher | : Springer Science & Business Media |
Total Pages | : 381 |
Release | : 2008-02-02 |
Genre | : Science |
ISBN | : 1592593968 |
Directed evolution comprises two distinct steps that are typically applied in an iterative fashion: (1) generating molecular diversity and (2) finding among the ensemble of mutant sequences those proteins that perform the desired fu- tion according to the specified criteria. In many ways, the second step is the most challenging. No matter how cleverly designed or diverse the starting library, without an effective screening strategy the ability to isolate useful clones is severely diminished. The best screens are (1) high throughput, to increase the likelihood that useful clones will be found; (2) sufficiently sen- tive (i. e. , good signal to noise) to allow the isolation of lower activity clones early in evolution; (3) sufficiently reproducible to allow one to find small improvements; (4) robust, which means that the signal afforded by active clones is not dependent on difficult-to-control environmental variables; and, most importantly, (5) sensitive to the desired function. Regarding this last point, almost anyone who has attempted a directed evolution experiment has learned firsthand the truth of the dictum “you get what you screen for. ” The protocols in Directed Enzyme Evolution describe a series of detailed p- cedures of proven utility for directed evolution purposes. The volume begins with several selection strategies for enzyme evolution and continues with assay methods that can be used to screen enzyme libraries. Genetic selections offer the advantage that functional proteins can be isolated from very large libraries s- ply by growing a population of cells under selective conditions.
Author | : Manfred T. Reetz |
Publisher | : John Wiley & Sons |
Total Pages | : 405 |
Release | : 2023-01-09 |
Genre | : Science |
ISBN | : 3527836888 |
Enzyme Engineering An authoritative and up-to-date discussion of enzyme engineering and its applications In Enzyme Engineering: Selective Catalysts for Applications in Biotechnology, Organic Chemistry, and Life Science, a team of distinguished researchers deliver a robust treatment of enzyme engineering and its applications in various fields such as biotechnology, life science, and synthesis. The book begins with an introduction to different protein engineering techniques, covers topics like gene mutagenesis methods for directed evolution and rational enzyme design. It includes industrial case studies of enzyme engineering with a focus on selectivity and activity. The authors also discuss new and innovative areas in the field, involving machine learning and artificial intelligence. It offers several insightful perspectives on the future of this work. Readers will also find: A thorough introduction to directed evolution and rational design as protein engineering techniques Comprehensive explorations of screening and selection techniques, gene mutagenesis methods in directed evolution, and guidelines for applying gene mutagenesis in organic chemistry, pharmaceutical applications, and biotechnology Practical discussions of protein engineering of enzyme robustness relevant to organic and pharmaceutical chemistry Treatments of artificial enzymes as promiscuous catalysts Various lessons learned from semi-rational and rational directed evolution A transdisciplinary treatise, Enzyme Engineering: Selective Catalysts for Applications in Biotechnology, Organic Chemistry, and Life Science is perfect for protein engineers, theoreticians, organic, and pharmaceutical chemists as well as transition metal researchers in catalysis and biotechnologists.
Author | : Young Je Yoo |
Publisher | : Springer |
Total Pages | : 208 |
Release | : 2017-01-12 |
Genre | : Technology & Engineering |
ISBN | : 9402410260 |
This book provides a comprehensive introduction to all aspects of enzyme engineering, from fundamental principles through to the state-of-the-art in research and industrial applications. It begins with a brief history, describing the milestones of advancement in enzyme science and technology, before going on to cover the fundamentals of enzyme chemistry, the biosynthesis of enzymes and their production. Enzyme stability and the reaction kinetics during enzymatic reactions are presented to show how enzymes function during catalysis and the factors that affect their activity. Methods to improve enzyme performance are also presented, such as cofactor regeneration and enzyme immobilization. The book emphasizes and elaborates on the performance and characteristics of enzymes at the molecular level. Finally, the book presents recent advances in enzyme engineering and some key industrial application of enzymes addressing the present needs of society. This book presents essential information not only for undergraduate and graduate students, but also for researchers in academia and industry, providing a valuable reference for the development of commercial applications of enzyme technology.
Author | : Ichiro Chibata |
Publisher | : Springer Science & Business Media |
Total Pages | : 520 |
Release | : 2013-03-14 |
Genre | : Science |
ISBN | : 1461592909 |
Presently, intensive and global attention is being devoted to "biotechnology"--the technology utilizing marvelous capacities of living things for human welfare. Each country is strongly promo ting its development. In particular, enzyme engineering, whose pur pose is to utilize efficiently enzymes, microorganisms, and cultured plant as well as animal cells as organic catalysts, is one of the main themes in the field of biotechnology. Under these circumstances, the Sixth Enzyme Engineering Confe rence was held at Kashikojima, Mie Prefecture, Japan from September 20 to 25, 1981, under the joint auspices of the Engineering Foun dation of New York and the Japanese Society of Enzyme Engineering. This series of international conferences has been held biannually since 1971. The first three and the fifth conferences were held in the United States and the fourth one was in the Federal Republic of Germany. This sixth conference was the first to be held in Asia; and it was significant that a number of participant£ could visit Japany, which has produced successful achievements in the field of biotechnology.
Author | : Andreas Vogel |
Publisher | : John Wiley & Sons |
Total Pages | : 430 |
Release | : 2019-10-28 |
Genre | : Science |
ISBN | : 3527343857 |
This reference is a "must-read": It explains how an effective and economically viable enzymatic process in industry is developed and presents numerous successful examples which underline the efficiency of biocatalysis.
Author | : Stephanie Shih-Min Lam |
Publisher | : |
Total Pages | : 184 |
Release | : 2016 |
Genre | : |
ISBN | : |
A thorough understanding of biological systems on a molecular level requires tools to study individual protein components within the native cellular context. A wide panoply of enzyme-mediated labeling methodologies have been developed that couple the power of enzyme specificity with the functional diversity of small molecules to specifically tag proteins within the crowded heterogeneous intracellular environment for a wide array of applications including imaging by fluorescence or electron microscopy, and isolation and identification by mass spectrometry. Towards this end, a common theme in the design of new enzyme tools has been the repurposing of enzymes away from their natural chemical substrates to derivatives that provide more useful properties such as fluorescence or high-affinity handles. Many current directed evolution methods focus on the detection of product formation, but have limited ability to address improvements in catalytic efficiency. As a result, many of the developed methods may work in permissive proof-of-concept demonstrations in model cell lines under overexpression conditions, but lack sufficient activity to generate detectable signal in more relevant biological systems in which enzyme expression levels are more restricted. The goal of this thesis is to explore the development and application of directed evolution methods towards the challenging problem of improving enzyme catalysis. We attempted to design a new phage-display directed evolution platform for improving enzyme turnover of the E. coli biotin ligase BirA, but found that our separation based on ultracentrifugation lacked the necessary sensitivity to discriminate between levels of enzyme turnover. We also looked at applications of the specific labeling of BirA and promiscuous labeling of horseradish peroxidase towards capturing fluorescent live cellular migration tracks on the traversed extracellular matrix. We then utilized yeast display to develop a selection method for evolving peroxidases with improved activity to address the sensitivity limitations of the previously developed enhanced ascorbate peroxidase (APEX) tag from the Ting lab used for electron microscopy and spatial proteomics. This led to the successful discovery and characterization of the highly-active APEX2 which enabled advancements in mitochondrial and mitochondria-endoplasmic reticulum junction biology.
Author | : Huimin Zhao |
Publisher | : John Wiley & Sons |
Total Pages | : 41 |
Release | : 2021-08-23 |
Genre | : Science |
ISBN | : 3527344705 |
A one-stop reference that reviews protein design strategies to applications in industrial and medical biotechnology Protein Engineering: Tools and Applications is a comprehensive resource that offers a systematic and comprehensive review of the most recent advances in the field, and contains detailed information on the methodologies and strategies behind these approaches. The authors—noted experts on the topic—explore the distinctive advantages and disadvantages of the presented methodologies and strategies in a targeted and focused manner that allows for the adaptation and implementation of the strategies for new applications. The book contains information on the directed evolution, rational design, and semi-rational design of proteins and offers a review of the most recent applications in industrial and medical biotechnology. This important book: Covers technologies and methodologies used in protein engineering Includes the strategies behind the approaches, designed to help with the adaptation and implementation of these strategies for new applications Offers a comprehensive and thorough treatment of protein engineering from primary strategies to applications in industrial and medical biotechnology Presents cutting edge advances in the continuously evolving field of protein engineering Written for students and professionals of bioengineering, biotechnology, biochemistry, Protein Engineering: Tools and Applications offers an essential resource to the design strategies in protein engineering and reviews recent applications.