Engineering Of Dna Mediated Assemblies For Biosensing Applications
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| Author | : Phyllis F. Xu |
| Publisher | : |
| Total Pages | : 97 |
| Release | : 2013 |
| Genre | : |
| ISBN | : 9781303379796 |
Traditionally known as the genetic code, in recent years DNA has been engineered in new ways and applied toward novel applications in materials science and bio-nanotechnology. The nanometer features and highly specific base-pairing of DNA has enabled its use to build complex 2- and 3D nano-architectures and nano-structures. Specific DNA scaffolds include branched DNA junctions, 2D and 3D DNA "tiles" and "bricks", and DNA hydrogels that can be used for applications across various fields of materials science. Another unique feature of DNA is its ability to bind other materials through molecular recognition which has made the use of DNA aptamer or DNA-protein conjugates highly useful for applications in biosensing and identification. The main focus of this thesis is to demonstrate the use of DNA as an invaluable tool for engineering specific nanostructures for biosensing. The first chapter introduces the basics of DNA and current research in various DNA structures and assemblies and their applications. The second and third chapters describe the self-assembly of two structurally different nanomaterials using DNA, carbon nanotubes and gold nanoparticles. In the second chapter, the successful large-scale alignment of carbon nanotubes on a surface is shown. The study goes into the factors that affect the quality of alignment, including salt concentration, length of DNA, and annealing. In the third chapter, we show how DNA can be used to engineer discrete gold nanocrystal assemblies-"nanodumbbells"- that can adopt different structural conformations through DNA interactions. By incorporating DNA aptamers in the nanoparticle structure, these nanodumbbells can be used to sense particular analytes in solution. Chapter 4 continues the study of the nanodumbbell structures and their potential use as surface-enhanced Raman spectroscopy (SERS) biosensors. A large measurable difference in Raman signal was experimentally obtained from the two distinct nanodumbbell conformations. This was compared with theoretical studies of SERS enhancement to validate the signals produced. We were also able to show the successful detection of an analyte, ATP, using the DNA-mediated probe and SERS detection method.
| Author | : Aaron Bruce Rabinowitz |
| Publisher | : |
| Total Pages | : 24 |
| Release | : 2000 |
| Genre | : |
| ISBN | : |
| Author | : Tony Jefferson Gnanaprakasa |
| Publisher | : |
| Total Pages | : 123 |
| Release | : 2010 |
| Genre | : Biosensors |
| ISBN | : |
| Author | : William A. Goddard III |
| Publisher | : CRC Press |
| Total Pages | : 1080 |
| Release | : 2007-05-03 |
| Genre | : Science |
| ISBN | : 142000784X |
The ability to study and manipulate matter at the nanoscale is the defining feature of 21st-century science. The first edition of the standard-setting Handbook of Nanoscience, Engineering, and Technology saw the field through its infancy. Reassembling the preeminent team of leading scientists and researchers from all areas of nanoscience and nanote
| Author | : Björn Högberg |
| Publisher | : |
| Total Pages | : 74 |
| Release | : 2007 |
| Genre | : |
| ISBN | : 9789185317394 |
| Author | : So-Jung Park |
| Publisher | : |
| Total Pages | : |
| Release | : 2002 |
| Genre | : |
| ISBN | : |
Based on this observation, a DNA array detection method was developed in which the binding of Au nanoparticles functionalized with oligonucleotides led to conductivity changes associated with target-probe binding events. The binding events localize gold nanoparticles in an electrode gap; silver deposition facilitated by these nanoparticles bridges the gap and leads to readily measurable conductivity changes. Using this method, we have detected target DNA at concentrations as low as 500 fM with a point mutation selectivity factor of ∼100,000:1. These unprecedented capabilities point towards a way of eliminating the need for polymerase chain reaction (PCR) in DNA detection.
| Author | : Jung-II Jin |
| Publisher | : CRC Press |
| Total Pages | : 349 |
| Release | : 2011-12-12 |
| Genre | : Medical |
| ISBN | : 1439827419 |
The field of materials science and technology has undergone revolutionary advances due to the development of novel analytical tools, functional materials, and multidisciplinary approaches to engineering. Additionally, theoretical predictions combined with increasingly improved models and computational capabilities are making impressive contributions to the progress of materials science and technology. In particular, the materials science of DNA has emerged as a vital area of research and is expected to immensely broaden the horizon of material science and nanotechnology in this century. Materials Science of DNA highlights the most important subjects and perspectives in the field, with the aim of stimulating the interdisciplinary community and bringing this intensively interesting, emerging field of molecular-scale materials science to maturation. The editors have not only been involved in the research of materials science of DNA for the past decade, but also lead the series of International Biotronics Workshops supported by the US Air Force Research Laboratory. Biotechnology and DNA-based biopolymers are not only applicable for genomic sequencing and clinical diagnosis and treatment, but can also have a major impact on nonbiotech applications—such as electronics and photonics— opening up a whole new field for bioengineering. New concepts and insights gained from DNA research are expected to prove genuinely useful in a variety of devices in nano, micro, and macro dimensions in the future. Where silicon has been the building block of inorganic electronics and photonics, DNA holds promise to become the building block for organic electronics and photonics.
| Author | : Genxi Li |
| Publisher | : Elsevier |
| Total Pages | : 380 |
| Release | : 2018-08-29 |
| Genre | : Science |
| ISBN | : 0128150548 |
Nano-inspired Biosensors for Protein Assay with Clinical Applications introduces the latest developments in nano-inspired biosensing, helping readers understand both the fundamentals and frontiers in this rapidly advancing field. In recent decades, there has been increased interest in nano-inspired biosensors for clinical application. Proteins, e.g. antigen-antibody, tumor markers and enzymes are the most important target in disease diagnosis, and a variety of biosensing techniques and strategies have been developed for protein assay. This book brings together all the current literature on the most recent advances of protein analysis and new methodologies in designing new kinds of biosensors for clinical diagnostic use. Provides a single source of information on the latest developments in the field of biosensors for protein analysis and clinical diagnosis Focuses on biosensors fabricated with nanomaterials and nanotechnology Gives detailed methodologies for designing and fabricating nano-inspired biosensors
| Author | : Thimmaiah Govindaraju |
| Publisher | : |
| Total Pages | : 0 |
| Release | : 2022 |
| Genre | : |
| ISBN | : 9789811641909 |
This book is the ultimate assembly of recent research activities on molecular architectonics and nanoarchitectonics by authors who are worldwide experts. The book proposes new ways of creating functional materials at the nano level using the concepts of molecular architectonics and nanoarchitectonics, which are expected to be the next-generation approaches beyond conventional nanotechnology. All the contents are categorized by types of materials, organic materials, biomaterials, and nanomaterials. For that reason, non-specialists including graduate and undergraduate students can start reading the book from any points they would like. Cutting-edge trends in nanotechnology and material sciences are easily visible in the contents of the book, which is highly useful for both students and experimental materials scientists. .
| Author | : William A. Goddard III |
| Publisher | : CRC Press |
| Total Pages | : 1097 |
| Release | : 2012-06-12 |
| Genre | : Technology & Engineering |
| ISBN | : 1439860157 |
In his 1959 address, "There is Plenty of Room at the Bottom," Richard P. Feynman speculated about manipulating materials atom by atom and challenged the technical community "to find ways of manipulating and controlling things on a small scale." This visionary challenge has now become a reality, with recent advances enabling atomistic-level tailoring and control of materials. Exemplifying Feynman’s vision, Handbook of Nanoscience, Engineering, and Technology, Third Edition continues to explore innovative nanoscience, engineering, and technology areas. Along with updating all chapters, this third edition extends the coverage of emerging nano areas even further. Two entirely new sections on energy and biology cover nanomaterials for energy storage devices, photovoltaics, DNA devices and assembly, digital microfluidic lab-on-a-chip, and much more. This edition also includes new chapters on nanomagnet logic, quantum transport at the nanoscale, terahertz emission from Bloch oscillator systems, molecular logic, electronic optics in graphene, and electromagnetic metamaterials. With contributions from top scientists and researchers from around the globe, this color handbook presents a unified, up-to-date account of the most promising technologies and developments in the nano field. It sets the stage for the next revolution of nanoscale manufacturing—where scalable technologies are used to manufacture large numbers of devices with complex functionalities.
