Cytochrome P450 Protocols
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Author | : Ian R. Phillips |
Publisher | : Springer Science & Business Media |
Total Pages | : 355 |
Release | : 2008-02-04 |
Genre | : Science |
ISBN | : 1592599982 |
For this second edition of their much praised Cytochrome P450, the editors have collected accounts of the essential core techniques that use the latest methodologies for the investigation of P450s. Highlights include protocols for spectral analysis and purification of P450s, enzymatic assays of P450s and flavin-containing monooxygenases (FMOs), expression of P450s and FMOs in heterologous systems, and the production and use of antipeptide antibodies. Additional chapters contain readily reproducible techniques for the transfection of hepatocytes for gene regulation studies, P450 reporter gene assays, in situ hybridization, and analysis of genetic polymorphisms. Although the emphasis is on P450s of mammalian origin, many of the readily reproducible methods described are suitable for P450s from any source.
Author | : Ian R. Phillips |
Publisher | : Humana Press |
Total Pages | : 0 |
Release | : 2013-03-09 |
Genre | : Science |
ISBN | : 9781627033206 |
Cytochromes P450 (CYPs) comprise a large superfamily of proteins that are of central importance in the detoxification or activation of a tremendous number of natural and synthetic hydrophobic xenobiotics, including many therapeutic drugs, chemical carcinogens and environmental pollutants. CYPs are important in mediating interactions between an organism and its chemical environment and in the regulation of physiological processes. Cytochrome P450 Protocols, Third Edition focuses on high-throughput methods for the simultaneous analysis of multiple CYPs, substrates or ligands. Although the emphasis is on CYPs of mammalian origin, it reflects an increasing interest in CYPs of bacterial species. Also included are chapters on cytochrome P450 reductase (the redox partner of CYPs) and the flavin-containing monooxygenases (FMOs), and metabolomic and lipidomic approaches for identification of endogenous substrates of CYPs (‘de-orphanizing’ CYP substrates). Written in the successful Methods in Molecular BiologyTM series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Cytochrome P450 Protocols, Third Edition provides a wide range of techniques accessible to researchers in fields as diverse as biochemistry, molecular biology, pharmacology, toxicology, environmental biology and genetics.
Author | : John M. Walker |
Publisher | : |
Total Pages | : |
Release | : 1984 |
Genre | : Molecular biology -- v.236. Plant functional genomics -- v.237. G protein signaling -- v.238. Biopolymer methods in tissue engineering -- v.239. Cell migration in inflammation and immunity -- v.240. Mammalian artificial chromosomes -- v.241. Cell cycle checkpoint control protocols -- v.242. Atomic force microscopy -- v.243. Chiral separations: Methods and protocols -- v.244. Protein purification protocols -- v.245-6. Gene delivery to mammalian cells -- v.247. Drosophila cytogenetics protocols -- v.248. Antibody engineering -- v.249. Cytokine protocols -- v.251. HPLC of peptides and proteins: Methods and protocols -- v.265. RNA interference, editing, and modification -- v.274. Photosynthesis research protocols -- v.318. Plant cell culture protocols -- v.323. Arabidopsis protocols |
ISBN | : |
Author | : David F.V. Lewis |
Publisher | : CRC Press |
Total Pages | : 344 |
Release | : 1996-07-03 |
Genre | : Science |
ISBN | : 1482272776 |
This book covers the relevant aspects of cytochromes P450 for understanding their structure, function and mechanisms of action, such as physicochemical characterization methods, redox interactions, evolution and genetics, experimental and theoretical models, induction, inhibition and metabolism.
Author | : |
Publisher | : |
Total Pages | : 379 |
Release | : 2021 |
Genre | : Cytochrome P-450 |
ISBN | : 9781071615447 |
Author | : Paul R. Ortiz de Montellano |
Publisher | : Springer Science & Business Media |
Total Pages | : 702 |
Release | : 2005 |
Genre | : Medical |
ISBN | : 0306483246 |
Since the first edition of this title in 1986, the cytochrome P450 field has completed the transition to a discipline in which structure and mechanism, even regulation and biological function, are dealt with in molecular terms. The present updated edition has dropped some of the chapter topics from the first edition to make room for new or expanded topics. The volume is divided into four sections: model systems; structurally defined bacterial enzymes; structures and mechanisms of membrane-bound P450 enzymes; and regulatory mechanisms and physiological roles of cytochrome P450.
Author | : Costas Ioannides |
Publisher | : CRC Press |
Total Pages | : 426 |
Release | : 1996-09-06 |
Genre | : Medical |
ISBN | : 9781439810705 |
Cytochromes P450: Metabolic and Toxicological Aspects examines cytochrome P450 proteins and their role in toxicity/carcinogenicity and the metabolism of foreign chemicals. Studying the function of these proteins enables us to: Predict the pathways and outcome of chemical metabolism to rationalize species, sex, and age differences in toxicity Anticipate drug interactions Modify doses to fit the needs of patients Contributions from internationally acknowledged experts are organized into three sections. The first section provides an overview, the next profiles each of the cytochrome P450 families and subfamilies involved in chemical metabolism, and the last section discusses new issues and developments of current interest. This detailed and thorough examination of cytochrome P450 will be a useful source for research scientists, especially those working in the pharmaceutical industry, dealing with the safety evaluation of chemicals and the study of their metabolism, pharmacokinetics, and toxicological properties.
Author | : David F.V. Lewis |
Publisher | : CRC Press |
Total Pages | : 344 |
Release | : 1996-07-03 |
Genre | : Science |
ISBN | : 0748404430 |
This guide to the structure, function and mechanism of the cytochromes P450 focuses on the role of P450s in xenobiotic metabolism and toxicity. Colour illustrations show how modelling of P450s can rationalize their substrate specificity for the metabolism of both endogenous and exogenous chemicals.
Author | : Eric F. Johnson |
Publisher | : Academic Press |
Total Pages | : 461 |
Release | : 2002 |
Genre | : Biochemistry |
ISBN | : 0121822605 |
Human genomics and genetics; Structure and mechanism; Regulation of expression; Metabolism; Invertebrate P450s.
Author | : David F. V. Lewis |
Publisher | : CRC Press |
Total Pages | : 240 |
Release | : 2019-12-02 |
Genre | : |
ISBN | : 9780367447205 |
This book focuses on human Cytochrome P450s and drug metabolism in Man. It describes the structures of the P450s, and reviews the methodology available to determine their nature, including molecular modelling, X-ray crystallography, spectroscopy, and molecular biology.