This book contains the Proceedings of the Seventh Meeting on CPT and Lorentz Symmetry, held at Indiana University in Bloomington on June 20-24, 2016. The meeting focused on tests of these fundamental symmetries and on related theoretical issues, including scenarios for possible violations.Topics covered at the meeting include experimental and observational searches for CPT and Lorentz violation involving: accelerator and collider experiments; astrophysical birefringence, dispersion, and anisotropy; atomic and molecular spectroscopy; clock-comparison measurements; CMB polarization; decays of atoms, nuclei, and particles; equivalence-principle tests with matter and antimatter; exotic atoms, muonium, positronium; gauge and Higgs particles; gravimetry; gravitational waves; high-energy astrophysical observations; hydrogen and antihydrogen; matter interferometry; neutrino oscillations and propagation, neutrino-antineutrino mixing; oscillations and decays of K, B, D mesons; particle-antiparticle comparisons; post-Newtonian gravity in the solar system and beyond; resonant cavities lasers; second and third-generation particles; sidereal and annual time variations, compass asymmetries; space-based missions; spin-polarized matter; spin precession; tests of short-range gravity; and time-of-flight measurements. Theoretical and phenomenological discussions include: physical effects at the level of the Standard Model, General Relativity, and beyond; origins and mechanisms for violations; classical and quantum field theory, gravitation, particle physics, and strings; mathematical foundations; and Finsler geometry.