Cell Surface Proteases
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| Author | : |
| Publisher | : Elsevier |
| Total Pages | : 475 |
| Release | : 2003-05-03 |
| Genre | : Science |
| ISBN | : 0080490883 |
Cell Surface Proteases provides a comprehensive overview of these important enzymes that catalyze the hydrolysis of a protein as it degrades to a simpler substance. In the 1990s, an explosion of new discoveries shed light on the role of cell surface proteases and extended it beyond degradation of extracellular matrix components to include its influence on growth factors, cell signaling, and other cellular events. This volume unites the scientific literature from across disciplines and teases out unified themes of interactions between cell surface proteases and interconnecting cell surface-related systems -- including integrins and other adhesion molecules. Scientists and students involved in developmental biology, cell biology and disease processes will find this an indispensable resource. * Provides an overview of the entire field of cell surface proteases in a single volume* Presents major issues and astonishing discoveries at the forefront of modern developmental biology and developmental medicine * A thematic volume in the longest-running forum for contemporary issues in developmental biology with over 30 years of coverage
| Author | : Eva Böttcher-Friebertshäuser |
| Publisher | : Springer |
| Total Pages | : 337 |
| Release | : 2018-05-22 |
| Genre | : Medical |
| ISBN | : 3319754742 |
This book will give an overview on viruses undergoing proteolytic activation through host proteases. The chapters will be organized in three themed parts, the first part describing respective viruses and their characteristics in detail. In the second part the molecular and cellular biology of the proteases involved as well as their physiological functions will be further explored. The third part will contain a chapter on protease inhibitors that are promising tools for antiviral therapy. This book will engage scholars in virology and medical microbiology as well as researchers with an interest in enzymology and protein structure and function relationship.
| Author | : Bruce Alberts |
| Publisher | : |
| Total Pages | : 0 |
| Release | : 2002 |
| Genre | : Cytology |
| ISBN | : 9780815332183 |
| Author | : Wolfgang B. Liedtke, MD, PH.D. |
| Publisher | : CRC Press |
| Total Pages | : 502 |
| Release | : 2006-09-29 |
| Genre | : Medical |
| ISBN | : 1420005847 |
Since the first TRP ion channel was discovered in Drosophila melanogaster in 1989, the progress made in this area of signaling research has yielded findings that offer the potential to dramatically impact human health and wellness. Involved in gateway activity for all five of our senses, TRP channels have been shown to respond to a wide range of st
| Author | : Klaudia Brix |
| Publisher | : Springer Science & Business Media |
| Total Pages | : 568 |
| Release | : 2014-01-21 |
| Genre | : Science |
| ISBN | : 3709108853 |
Proteolysis is an irreversible posttranslational modification affecting each and every protein from its biosynthesis to its degradation. Limited proteolysis regulates targeting and activity throughout the lifetime of proteins. Balancing proteolysis is therefore crucial for physiological homeostasis. Control mechanisms include proteolytic maturation of zymogens resulting in active proteases and the shut down of proteolysis by counteracting endogenous protease inhibitors. Beyond the protein level, proteolytic enzymes are involved in key decisions during development that determine life and death – from single cells to adult individuals. In particular, we are becoming aware of the subtle role that proteases play in signaling events within proteolysis networks, in which the enzymes act synergistically and form alliances in a web-like fashion. Proteases come in different flavors. At least five families of mechanistically distinct enzymes and even more inhibitor families are known to date, many family members are still to be studied in detail. We have learned a lot about the diversity of the about 600 proteases in the human genome and begin to understand their physiological roles in the degradome. However, there are still many open questions regarding their actions in pathophysiology. It is in this area where the development of small molecule inhibitors as therapeutic agents is extremely promising. Approaching proteolysis as the most important, irreversible post-translational protein modification essentially requires an integrated effort of complementary research disciplines. In fact, proteolytic enzymes seem as diverse as the scientists working with these intriguing proteins. This book reflects the efforts of many in this exciting field of research where team and network formations are essential to move ahead.
| Author | : |
| Publisher | : |
| Total Pages | : 44 |
| Release | : 2016 |
| Genre | : Electronic books |
| ISBN | : |
Proteolysis is one of the most essential biological processes as it serves different purposes such as protein maturation, degradation, and regulation. Proteolysis at the cell surface and extracellular matrix (ECM) plays significant roles in the progression of certain diseases as exemplified by the role of Matrix Metalloproteinase 14 (MMP-14) in cancer. MMP-14 is a ubiquitously-expressed cell surface protease known to activate several soluble MMPs (EC-MMPs) via extracellular proteolysis. Active EC-MMPs are then used for the restructuring of the ECM that can facilitate cell migration and development. MMP-14 is known to be highly expressed in cancer tissues, as cells require constant remodeling of the ECM to accommodate fast replication and migration that leads to metastasis. The aim of this research is to develop a novel cell-based assay for the monitoring of proteolytic cleavage on the cell surface. We propose to develop such assay using MMP-14 cleavage activity as a proof-of-principle. The assay could also serve as a screening tool for the search of novel MMP-14 inhibitors. The assay is based on a two-tag system flanking an optimized substrate of MMP-14 that serves as the primary detection element that can distinguish cleavage and non-cleavage events. A fluorescent protein is later introduced as a visual marker for cell surface localization by fluorescence microscopy. Lastly, the entire scaffold protein will be targeted and anchored on the cell surface by the addition of the C-terminal transmembrane domain of mouse Lyt-2 cell surface protein. The utility of the assay for monitoring cleavage on the cell surface will be demonstrated with the overexpression of MMP-14 as a proof-of-principle. Once the assay is calibrated and optimized to function as intended, it could also be further used as a platform to monitor other biologically important surface proteolysis events such as cleavage of the Amyloid Precursor Protein (APP) in Alzheimer disease and proteolysis of sialic acid by the influenza virus neuraminidase (NA) for the spread of viral particles. More importantly, it can also be adapted to a high-throughput screening (HTS) platform for the screening for novel inhibitors of those aforementioned proteases.
| Author | : Ajit Varki |
| Publisher | : CSHL Press |
| Total Pages | : 694 |
| Release | : 1999 |
| Genre | : Medical |
| ISBN | : 9780879696818 |
Sugar chains (glycans) are often attached to proteins and lipids and have multiple roles in the organization and function of all organisms. "Essentials of Glycobiology" describes their biogenesis and function and offers a useful gateway to the understanding of glycans.
| Author | : Robert Jerrold Fulton |
| Publisher | : |
| Total Pages | : 318 |
| Release | : 1980 |
| Genre | : |
| ISBN | : |
| Author | : Erwin E. Sterchi |
| Publisher | : Springer Science & Business Media |
| Total Pages | : 371 |
| Release | : 2013-11-11 |
| Genre | : Science |
| ISBN | : 3642598161 |
Following an overview on proteolytic enzyme assays, this text covers procedures on how to investigate and study proteases. It describes the use of specific restriction proteases as well as inhibitors of proteases to prevent unwanted proteolysis.
| Author | : Vito Turk |
| Publisher | : Birkhäuser |
| Total Pages | : 238 |
| Release | : 2012-12-06 |
| Genre | : Science |
| ISBN | : 303488737X |
This book provides an account of the recent advances in our understanding of the role of proteases under physiological and pathological conditions. It reviews the contributions that have been made in the fields of biochemistry, molecular biology, medicine, agriculture and ecology. In addition there is an account of the growing number of practical applications in biotechnology, reflecting the fact that peptidases are major targets for medical and agricultural purposes. The book shows how the numerous protease structures are essential for drug design. The contributions place special emphasis on cysteine, aspartic and metalloproteases and their role in physiological and certain pathological states. Another focus is the classification and nomenclature of peptidases and a review of those proteases currently under the most intensive investigation. The book provides an informative introduction for teachers and newcomers to the field, such as graduate students, while providing a valuable source of material and ideas for the academic and industrial researcher in areas including biochemistry, medicine, agriculture and biotechnology.
