Biochemistry Laboratory
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| Author | : Timea Gerczei Fernandez |
| Publisher | : Walter de Gruyter GmbH & Co KG |
| Total Pages | : 190 |
| Release | : 2015-03-11 |
| Genre | : Science |
| ISBN | : 3110426250 |
Biochemistry laboratory manual for undergraduates – an inquiry based approach by Gerczei and Pattison is the first textbook on the market that uses a highly relevant model, antibiotic resistance, to teach seminal topics of biochemistry and molecular biology while incorporating the blossoming field of bioinformatics. The novelty of this manual is the incorporation of a student-driven real real-life research project into the undergraduate curriculum. Since students test their own mutant design, even the most experienced students remain engaged with the process, while the less experienced ones get their first taste of biochemistry research. Inclusion of a research project does not entail a limitation: this manual includes all classic biochemistry techniques such as HPLC or enzyme kinetics and is complete with numerous problem sets relating to each topic.
| Author | : Rodney F. Boyer |
| Publisher | : Pearson |
| Total Pages | : 0 |
| Release | : 2012 |
| Genre | : Biochemistry |
| ISBN | : 9780136043027 |
The biochemistry laboratory course is an essential component in training students for careers in biochemistry, molecular biology, chemistry, and related molecular life sciences such as cell biology, neurosciences, and genetics. Increasingly, many biochemistry lab instructors opt to either design their own experiments or select them from major educational journals. Biochemistry Laboratory: Modern Theory and Techniques addresses this issue by providing a flexible alternative without experimental protocols. Instead of requiring instructors to use specific experiments, the book focuses on detailed descriptions of modern techniques in experimental biochemistry and discusses the theory behind such techniques in detail. An extensive range of techniques discussed includes Internet databases, chromatography, spectroscopy, and recombinant DNA techniques such as molecular cloning and PCR. The Second Edition introduces cutting-edge topics such as membrane-based chromatography, adds new exercises and problems throughout, and offers a completely updated Companion Website.
| Author | : Benjamin F. Lasseter |
| Publisher | : CRC Press |
| Total Pages | : 161 |
| Release | : 2019-09-30 |
| Genre | : Medical |
| ISBN | : 0429957378 |
Most lab manuals assume a high level of knowledge among biochemistry students, as well as a large amount of experience combining knowledge from separate scientific disciplines. Biochemistry in the Lab: A Manual for Undergraduates expects little more than basic chemistry. It explains procedures clearly, as well as giving a clear explanation of the theoretical reason for those steps. Key Features: Presents a comprehensive approach to modern biochemistry laboratory teaching, together with a complete experimental experience Includes chemical biology as its foundation, teaching readers experimental methods specific to the field Provides instructor experiments that are easy to prepare and execute, at comparatively low cost Supersedes existing, older texts with information that is adjusted to modern experimental biochemistry Is written by an expert in the field This textbook presents a foundational approach to modern biochemistry laboratory teaching together with a complete experimental experience, from protein purification and characterization to advanced analytical techniques. It has modules to help instructors present the techniques used in a time critical manner, as well as several modules to study protein chemistry, including gel techniques, enzymology, crystal growth, unfolding studies, and fluorescence. It proceeds from the simplest and most important techniques to the most difficult and specialized ones. It offers instructors experiments that are easy to prepare and execute, at comparatively low cost.
| Author | : Alexander J. Ninfa |
| Publisher | : John Wiley & Sons |
| Total Pages | : 480 |
| Release | : 2009-05-26 |
| Genre | : Science |
| ISBN | : 0470087668 |
Ninfa/Ballou/Benore is a solid biochemistry lab manual, dedicated to developing research skills, allowing students to learn techniques and develop the the critical thinking and organizational approaches necessary to conduct laboratory research. Ninfa/Ballou/Benore focuses on basic biochemistry laboratory techniques but also includes molecular biology exercises, a reflection of most courses which concentrate on traditional biochemistry experiments and techniques. The experiments are designed so that theory and technique are learned as fundamental research tools, and the biochemistry and molecular biology applications are seamlessly integrated throughout the manual. The manual also includes an introduction to ethics in the laboratory, uncommon in similar manuals. Most importantly, perhaps, is the authors’ three-pronged approach to encouraging students to think like a research scientist: first, the authors introduce the scientific method and the hypothesis as a framework for developing conclusive experiments; second, the manual’s experiments are designed to become increasingly complex in order to teach more advanced techniques and analysis; finally, gradually, the students are required to devise their own protocols. In this way, students and instructors are able to break away from a “cookbook” approach and to think and investigate for themselves. Suitable for lower-level and upper-level courses; Ninfa spans these courses and can also be used for some first-year graduate work.
| Author | : William G. O'Neal |
| Publisher | : |
| Total Pages | : 392 |
| Release | : 2020 |
| Genre | : |
| ISBN | : 9781640430440 |
This full-color, comprehensive, affordable manual is intended for a one-semester general, organic, and biochemistry course, preparatory/basic chemistry course, liberal arts chemistry course, or allied health chemistry course. The procedures are written with the goal of simplifying a complicated and often challenging subject for students by applying concepts to everyday life.The first half of the lab manual covers general topics such as chemical and physical properties, elements of the periodic table, types of bonds, empirical formulas, and reaction stoichiometry. These labs form the foundation for future labs, which cover the basics of organic and biological chemistry. Experiments include the classification of organic compounds and the determination of biomolecules. By the end of this course, students should have a solid understanding of the basic concepts of chemistry, which will give them confidence as they embark on various allied health careers.Features: ?Initiate the study of basic concepts in the general, organic, and biochemistry laboratory by reading through concise introductory material and answering pre-lab questions that familiarize students with the concepts presented in each exercise. The inclusion of color photography and high-quality art promotes engagement and comprehension of the more difficult concepts.?Investigate the mysteries of matter by following the clearly written procedures and recording data and observations on the provided data sheets. Common techniques are reviewed as needed in Technique Tips boxes to reinforce the development of basic laboratory skills. OSHA pictograms, and Lab Safety boxes are provided to help students understand any risks associated with specific chemicals and equipment.?Integrate knowledge of each laboratory topic by making sense of the data that has been collected. Reflective Exercises galvanize critical thinking and scientific analysis skills to take shape as students make connections between what has been learned and practiced in the hands-on lab and how this knowledge can be applied to a relevant, real-world context.
| Author | : William G. O'Neal |
| Publisher | : Morton Publishing Company |
| Total Pages | : 627 |
| Release | : 2017-02-01 |
| Genre | : Science |
| ISBN | : 1617316199 |
This full-color, comprehensive, affordable manual is appropriate for two-semester introductory chemistry courses. It is loaded with clearly written exercises, critical thinking questions, and full-color illustrations and photographs, providing ample visual support for experiment set up, technique, and results.
| Author | : Nenad Blau |
| Publisher | : Springer Science & Business Media |
| Total Pages | : 860 |
| Release | : 2008-05-31 |
| Genre | : Medical |
| ISBN | : 3540766987 |
This manual deals specifically with laboratory approaches to diagnosing inborn errors of metabolism. The key feature is that each chapter is sufficiently detailed so that any individual can adopt the described method into their own respective laboratory.
| Author | : André Picot |
| Publisher | : John Wiley & Sons |
| Total Pages | : 344 |
| Release | : 1996-12-17 |
| Genre | : Science |
| ISBN | : 9780471185567 |
Chemical and biochemical Laboratories are full of potentially dangerous chemicals and equipment. 'Safety in the Chemistry and Biochemistry Laboratory' provides the necessary information needed for working with these chemicals and apparatus to avoid: fires, explosions, toxic fumes, skin burns, poisoning and other hazards. Both authors, André Picot and Philippe Grenouillet, are recognized authorities in the field of lab safety, and their book arrange the information not available in similar publications. It is addressed to members of Chemical Health& Safety as well as working chemists in labs everywhere. Also Lab managers will find the book a useful addition to their bookshelf.
| Author | : Swati Agarwal |
| Publisher | : I K International Pvt Ltd |
| Total Pages | : 166 |
| Release | : 2018-08-10 |
| Genre | : Medical |
| ISBN | : 9385909967 |
This book provides detailed information on various instruments, techniques and experiment protocols of biochemistry and molecular biology. It deals with basic as well as advanced information and in-depth methodology in simple language to help students and professionals to perform experiments with ease. This book not only clears the practical concepts of Biochemistry and Molecular Biology at undergraduate and post-graduation levels, but also helps to pass the Ph.D. course work exam conducted by various universities. This book will develop research aptitude to clear the NET examination. This manual gives a comprehensive idea about the various instruments, their working, troubleshooting and their applications. It provides a wide spectrum of 14 chapters covering basic as well as advanced techniques and instrumentation, viz., Gas Chromatography (GC), Mass Spectrometry (MS), Scanning Electron Microscope (SEM), X-Ray Diffraction (XRD) and Fourier Transform Infrared Spectroscopy (FTIR) with detailed protocols. Most of the experiments can be easily performed in the laboratory having basic facilities. Historical background, experiment nature, its principle, step-by-step procedure with diagrammatic representation and important precautions are given in the beginning of each experiment.
| Author | : E. Carafoli |
| Publisher | : Springer Science & Business Media |
| Total Pages | : 187 |
| Release | : 2012-12-06 |
| Genre | : Science |
| ISBN | : 3642675301 |
This manual collects in the form of laboratory protocols a series of experiments in the field of Membrane Transport and Membrane Bioenergetics. It represents the experience accumulated during four advanced courses held at the Depart ment of Biochemistry of the Swiss Federal Institute of Technology on behalf of Federation of European Biochemical Societies (FEBS) in the years 1975 through 1978. The idea of collecting the experiments into a laboratory manual developed as a response to a demand from the students who took part in the courses. Further motivation came with the fmding that, in planning the laboratory sessions, the teaching staff had no organized, modern source of information in the literature. The experiments presented cover most areas of importance in the subject mat ter. Their presentation has been continuously modified in the course of the four years during which the manual took shape, to accommodate to experience and various suggestions. In their present form, all of the experiments described have been repeatedly practiced to optimize their execution. Efforts have been made to combine in the manual classical experiments, and techniques which require relatively unsophisticated instrumentation and can therefore be carried out in most laboratories, with more modern experiments and relatively newer technol ogies. In its present form, the manual should therefore provide a usefui tool in the hands of researchers and laboratory teachers at different levels of sophisti cation and instrumentation.
