Bioaffinity Chromatography

Bioaffinity Chromatography
Author: J. Turková
Publisher: Elsevier
Total Pages: 819
Release: 1993-09-14
Genre: Science
ISBN: 0080858635

Bioaffinity chromatography is now the preferred choice for the purification, determination or removal of many biologically active substances. The book includes information on biologically active substances with their affinants, solid supports and methods of coupling, summarized in tables covering classical, high-performance liquid and large-scale bioaffinity chromatography.Optimization of the preparation and the use of highly active and stable biospecific adsorbents is discussed in several chapters. Following a chapter dealing with the choice of affinity ligands, affinity-sorbent bonding is described in detail. Other chapters give information on solid supports, the most common coupling procedures and a general discussion of sorption and elution. Several applications of bioaffinity chromatography are described, e.g. quantitative evaluation of biospecific complexes and many applications in medicine and in the biotechnology industry.

Affinity Chromatography

Affinity Chromatography
Author: J. Turková
Publisher: Elsevier
Total Pages: 417
Release: 1978-01-01
Genre: Science
ISBN: 0080858120

Bioaffinity chromatography has increasingly become the method of choice for the purification, determination or removal of many biologically active substances.

Liquid Chromatography

Liquid Chromatography
Author: D.S. Hage
Publisher: Elsevier Inc. Chapters
Total Pages: 40
Release: 2013-01-08
Genre: Science
ISBN: 0128071060

Affinity chromatography is a type of liquid chromatography that uses a biologically related agent as a stationary phase to purify or analyze specific sample components. This chapter examines the basic components and principles of affinity chromatography and various applications of this method. The general types of ligands, immobilization schemes, support materials, and elution conditions that can be employed in affinity chromatography are first examined. Various methods in this field are then considered, including bioaffinity chromatography, immunoaffinity chromatography, dye-ligand and biomimetic affinity chromatography, immobilized metal-ion affinity chromatography, and analytical affinity chromatography. The types of ligands utilized in each of these methods are described, along with common applications of these techniques. Several related developments are also discussed, such as the use of affinity chromatography with mass spectrometry, miniaturized systems, molecularly imprinted polymers, and aptamers.

Liquid Chromatography

Liquid Chromatography
Author: Salvatore Fanali
Publisher: Newnes
Total Pages: 683
Release: 2013-01-08
Genre: Science
ISBN: 0124158668

A single source of authoritative information on all aspects of the practice of modern liquid chromatography suitable for advanced students and professionals working in a laboratory or managerial capacity - Chapters written by authoritative and visionary experts in the field provide an overview and focused treatment of a single topic - Each chapter emphasizes the integration of chromatographic methods and sample preparation, automation, and explains how liquid chromatography is used in different industrial sectors - Focuses on expanding and illustrating the main features of the fundamental section, while demonstrating where and how the best practices of liquid chromatography are utilized - Comprehensive coverage of modern liquid chromatography from theory, to methods, to selected applications - Thorough selected references and tables with commonly used data to facilitate research, practical work, comparison of results, and decision making

Protein Liquid Chromatography

Protein Liquid Chromatography
Author: M. Kastner
Publisher: Elsevier
Total Pages: 978
Release: 2000
Genre: Science
ISBN: 9780444502117

Protein Liquid Chromatography is a handbook-style guide to liquid chromatography as a tool for isolating and purifying proteins, consisting of 25 individual chapters divided into three parts: Part A covers commonly-used, classic modes of chromatography such as ion-exchange, size-exclusion, and reversed-phase; Part B deals with various target protein classes such as membrane proteins, recombinant proteins, and glycoproteins; and Part C looks at various miscellaneous related topics, including coupling reaction, buffer solution additives, and software. The text as a whole can be viewed as a systematic survey of available methods and how best to use them, but also attempts to provide an exhaustive coverage of each facet. How to solve a specific problem using a chosen method is the overall essence of the volume. The principle philosophy of this compilation is that practical application is everything; therefore, both classical and modern methods are presented in detail, with examples involving conventional, medium- and high-pressure techniques. Over-exposure to history, concept, and theory has deliberately been avoided. The reader will find a wealth of tips and tricks from users for users, including advice on the advantages and disadvantages of each method. Easy-to-read sections on "Getting started now" and "Where to go from here" attempt to provide hands-on, fool-proof detailed practical procedures with complete and even standard model runs for any scientist or technician at work in this area.

Encyclopedia of Chromatography

Encyclopedia of Chromatography
Author: Jack Cazes
Publisher: CRC Press
Total Pages: 996
Release: 2005
Genre: Science
ISBN: 9780824727864

A convenient source of information for workers in analytical chemistry, experimental biology, physics, and engineering, this Second Edition stands as a quick reference source and clear guide to specific chromatographic techniques and principles-providing a basic introduction to the science and technology of the method, as well as additional references on the theory and methodology for analysis of specific chemicals and applications in a range of industries.

Analytical and Preparative Separation Methods of Biomacromolecules

Analytical and Preparative Separation Methods of Biomacromolecules
Author: Hassan Y. Aboul-Enein
Publisher: CRC Press
Total Pages: 482
Release: 2020-10-15
Genre: Science
ISBN: 1000146251

Reports up-to-date research developments on purifying and isolation large organic molecules. The text provides information on high-performance liquid chromatography and capillary electrophoresis (CE) as tools for analyzing biomacromolecules and developing new biochemical and medicinal compounds. It applies biochemical separation technology to the study of macromolecules such as proteins, polysaccharides, nucleic acids and more.