This report presents a cost analysis of bio-based 1,4-Butanediol (BDO) production from glucose syrup using a direct fermentation process. The process examined is similar to Genomatica process. In this process, a 70 wt% glucose-water syrup is used as raw material. This report examines one-time costs associated with the construction of a United States-based plant and the continuing costs associated with the daily operation of such a plant. More specifically, it discusses: * Capital Investment, broken down by: - Total fixed capital required, divided in production unit (ISBL); infrastructure (OSBL) and contingency - Alternative perspective on the total fixed capital, divided in direct costs, indirect costs and contingency - Working capital and costs incurred during industrial plant commissioning and start-up * Production cost, broken down by: - Manufacturing variable costs (raw materials, utilities) - Manufacturing fixed costs (maintenance costs, operating charges, plant overhead, local taxes and insurance) - Depreciation and corporate overhead costs * Raw materials consumption, products generation and labor requirements * Process block flow diagram and description of industrial site installations (production unit and infrastructure) This report was developed based essentially on the following reference(s): (1) US Patent 20130109069, issued to Genomatica in 2013; (2) US Patent 8597918, issued to Genomatica in 2013 Keywords: BDO, Dextrose, Glucose Fermentation, Aerobic Fermentation, Green Butanediol, Renewable Feedstock