An Insect Cuticle Preparation Method For Light Microscopy
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Manual of Techniques in Insect Pathology
| Author | : Lawrence A. Lacey |
| Publisher | : Academic Press |
| Total Pages | : 441 |
| Release | : 1997-02-27 |
| Genre | : Technology & Engineering |
| ISBN | : 0080535771 |
Biological Techniques is a series of volumes aimed at introducing to a wide audience the latest advances in methodology. The pitfalls and problems of new techniques are given due consideration, as are those small but vital details not always explicit in the methods sections of journal papers.In recent years, most biological laboratories have been invaded by computers and a wealth of new DNA technology and this will be reflected in many of the titles appearing in the series.The books will be of value to advances researches and graduate students seeking to learn and apply new techniques, and will be useful to teachers of advanced undergraduate courses involving practical or project work.This manual describes the broad array of techniques that are used in insect pathology. It will provide biologists, insect pathologists, entomologists, and those interested in biological control, with the necessary information to work on a variety of pathogen groups.This book will be an essential laboratory reference for insect pathologists.Features include:* Step by-step instructions on how to isolate, identify, culture, bioassay and store the major groups of entomopathogens* Details of the practical knowledge needed by beginners to apply the techniques* Chapters written by an international group of experts* Discussion of safety testing of entomopathogens in mammals and also broader methods such as microscopy and molecular techniques* Provides extensive supplemental literature and recipes for media, fixatives and stains
Physiology of Ticks
| Author | : Frederick D. Obenchain |
| Publisher | : Elsevier |
| Total Pages | : 523 |
| Release | : 2013-10-22 |
| Genre | : Science |
| ISBN | : 1483162346 |
Physiology of Ticks focuses on the unique (and probably the most vulnerable) features of tick physiology and the physiological aspects of tick interactions with their hosts. The mechanisms used by non-feeding ticks to maintain their water balance are examined, along with the salivary mechanisms used by feeding ixodid ticks for excreting the enormous excess volumes of water and salts taken in during blood sucking. This book is comprised of 13 chapters and begins with a description of the morphology, deposition, and components of the tick cuticle. The discussion then turns to humidity relationships and water balance of ticks, as well as the sensory basis of tick feeding behavior and the immunological basis of host resistance to ticks. Subsequent chapters explore blood digestion in ticks; tick reproduction, with emphasis on sperm development, cytogenetics, oogenesis, and oviposition; effects of insect hormones and their mimics on tick development and reproduction; and the mechanisms of tick pheromones. The final chapter deals with diapause and biological rhythms in ticks. This monograph will be of value to entomologists, physiologists, biologists, and practitioners of tropical science.
Manual of Techniques in Invertebrate Pathology
| Author | : Lawrence A. Lacey |
| Publisher | : Academic Press |
| Total Pages | : 513 |
| Release | : 2012-04-25 |
| Genre | : Science |
| ISBN | : 0123869005 |
Manual of Techniques in Invertebrate Pathology, Second Edition, describes a wide range of techniques used in the identification, isolation, propagation/cultivation, bioassay, quantification, preservation, and storage of the major groups of entomopathogens, including entomophthorales, entomopathogenic fungi, entomopathogenic bacteria of the Bacilli, Nematode parasites, and pathogens and parasites of terrestrial molluscs. The book presents the perspectives of an international group of experts in the fields of invertebrate pathology, including microbiology, mycology, virology, nematology, biological control, and integrated pest management. Organized into 15 chapters, the book covers methods for the study of virtually every major group of entomopathogen, as well as methods for discovery and diagnosis of entomopathogens and the use of complementary methods for microscopy. It discusses the use of molecular techniques for identifying and determining phylogeny, factors that contribute to resistance to entomopathogens, and several other aspects of the science of invertebrate pathology. It also explains initial handling and diagnosis of diseased invertebrates, basic techniques in insect virology, and bioassay of bacterial entomopathogens against insect larvae. In addition, the reader is introduced to the use of bacteria against soil-inhabiting insects and preservation of entomopathogenic fungal cultures. The remaining chapters focus on research methods for entomopathogenic microsporidia and other protists, how the pathogenicity and infectivity of entomopathogens to mammals are tested, and preparations of entomopathogens and diseased specimens for more detailed study using microscopy. Experienced insect pathologists, biologists, entomologists, students, biotechnology personnel, technicians, those working in the biopesticide industry, and government regulators will find this manual extremely helpful. - Step-by-step instructions for the latest techniques on how to isolate, identify, culture, bioassay and store the major groups of entomopathogens - New edition fully updated to address changes in the taxonomy of the vast majority of taxa - Discussion of safety testing of entomopathogens in mammals and also broader methods such as microscopy and molecular techniques - Provides extensive supplemental literature and recipes for media, fixatives and stains
Microscopic-analytical Methods in Food and Drug Control
| Author | : United States. Food and Drug Administration |
| Publisher | : |
| Total Pages | : 300 |
| Release | : 1960 |
| Genre | : Drug adulteration |
| ISBN | : |
Environmental Physiology and Biochemistry of Insects
| Author | : K. H. Hoffmann |
| Publisher | : Springer Science & Business Media |
| Total Pages | : 304 |
| Release | : 2012-12-06 |
| Genre | : Science |
| ISBN | : 3642700209 |
Of all the zoological classes the insects are the most numerous in species and the most varied in structure. Estimates of the number 18 of species vary from 1 to 10 million, and 10 individuals are es timated to be alive at any given moment. In their evolution, in sects are relatively ancient and, therefore, they have proved to be a phenomenally successful biological design which has survived unchanged in its basic winged form during the last 300 m. y. In sects were the first small animals to colonize the land with full suc cess. Their small size opened many more ecological niches to them and permitted a greater diversification than the vertebrates. What is it about this design that has made insects so successful in habitats stretching from arid deserts to the Arctic and Antarctic and from freshwater brooks to hot springs and salines? Is it due to the adapta bility of their behavior, physiology, and biochemistry to changing environmental conditions? Three features of insects are of particular importance in determin ing their physiological relationship with the environment: their small size, as mentioned above, the impermeability and rigidity of their exoskeleton, and their poikilothermy. Of course, as with any other animals, the insects' success in its environment depends on its ability to maintain its internal state within certain tolerable limits of temperature, osmotic pressure, pH or oxygen concentra tion (homoeostasis).
Cuticle Techniques in Arthropods
| Author | : T. A. Miller |
| Publisher | : Springer Science & Business Media |
| Total Pages | : 422 |
| Release | : 2012-12-06 |
| Genre | : Science |
| ISBN | : 1461260760 |
Insects as a group occupy a middle ground in the biosphere between bac teria and viruses at one extreme, amphibians and mammals at the other. The size and general nature of insects present special problems to the student of entomology. For example, many commercially available in struments are geared to measure in grams, while the forces commonly en countered in studying insects are in the milligram range. Therefore, tech niques developed in the study of insects or in those fields concerned with the control of insect pests are often unique. Methods for measuring things are common to all sciences. Advances sometimes depend more on how something was done than on what was measured; indeed a given field often progresses from one technique to another as new methods are discovered, developed, and modified. Just as often, some of these techniques find their way into the classroom when the problems involved have been sufficiently ironed out to permit students to master the manipulations in a few laboratory periods. Many specialized techniques are confined to one specific research labo ratory. Although methods may be considered commonplace where they are used, in another context even the simplest procedures may save con siderable time. It is the purpose of this series (1) to report new develop ments in methodology, (2) to reveal sources of groups who have dealt with and solved particular entomological problems, and (3) to describe ex periments which might be applicable for use in biology laboratory courses.
Advanced Techniques in Biological Electron Microscopy
| Author | : J.K. Koehler |
| Publisher | : Springer Science & Business Media |
| Total Pages | : 319 |
| Release | : 2012-12-06 |
| Genre | : Science |
| ISBN | : 3642654924 |
The past decade has seen a remarkable increase in the use of electron microscopy as a researm tool in biology and medicine. Thus, most institu tions of higher learning now boast several electron optical laboratories having various levels of sophistication. Training in the routine use of elec tron optical equipment and interpretation of results is no longer restricted to a few prestigious centers. On the other hand, temniques utilized by researm workers in the ultrastructural domain have become extremely diverse and complex. Although a large number of quite excellent volumes of electron microscopic temnique are now dedicated to the basic elements available whim allow the novice to acquire a reasonable introduction to the field, relatively few books have been devoted to a discussion of more ad vanced temnical aspects of the art. It was with this view that the present volume was conceived as a handy reference for workers already having some background in the field, as an information source for those wishing to shift efforts into more promising temniques, or for use as an advanced course or seminar guide. Subject matter has been mosen particularly on the basis of pertinence to present researm activities in biological electron microscopy and emphasis has been given those areas whim seem destined to greatly expand in useful ness in the near future.
