The second edition of the Carbon Sequestration Atlas of the United States and Canada contains three main sections: (1) Introduction, (2) National Perspectives, and (3) Regional Perspectives. The Introduction section contains an overview of CCS technologies, a summary of the DOE's efforts in the CCS area, a brief description of the RCSP Program, and information on the National Carbon Sequestration Database and Geographic Information System (NATCARB). The National Perspectives section provides maps showing the number, location, and magnitude of identified CO2 stationary sources in the U.S. and portions of Canada, as well as the areal extent and estimated CO2 storage resource available in geologic formations evaluated within the RCSP Regions. The National Perspectives section also contains a summary of the methodologies and assumptions employed to calculate CO2 emissions and estimated CO2 storage resource of various geologic formations. The Regional perspectives section includes a detailed presentation of CO2 stationary sources, CO2 storage resource assessments, updates on field projects, and information on commercialization opportunities in each RCSP based on these methodologies and assumptions.