Two Phase Systems
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| Author | : Rajni Hatti-Kaul |
| Publisher | : Springer Science & Business Media |
| Total Pages | : 442 |
| Release | : 2008-02-05 |
| Genre | : Science |
| ISBN | : 1592590284 |
A mixture of two polymers, or one polymer and a salt, in an aqueous medium separates into two phases: this phenomenon is useful in biotechn- ogy for product separations. Separation of biological molecules and particles in these aqueous two-phase systems (ATPS) was initiated over 40 years ago by P.-Å. Albertsson, and later proved to be of immense utility in biochemical and cell biological research. A boost in the application of ATPS was seen when problems of separations in biotechnology processes were encountered. Its simplicity, biocompatibility, and amenability to easy scaleup operations make the use of ATPS very attractive for large-scale bioseparations. Despite the advantages ATPS enjoys over other separation techniques, the application of two-phase systems has for a long time been confined to selected labora- ries. Recent years have, however, shown a trend in which increasing numbers of researchers employ two-phase partitioning techniques in both basic and applied research.
| Author | : Harry Walter |
| Publisher | : Elsevier |
| Total Pages | : 731 |
| Release | : 2012-12-02 |
| Genre | : Science |
| ISBN | : 0323142915 |
Partitioning in Aqueous Two-Phase Systems: Theory, Methods, Uses, and Applications to Biotechnology is a collection of papers that discusses the applications of aqueous two-phase systems to problems of separation and extraction of macromolecules, organelles, and cells. Papers focus on the theoretical basis and the practical details of the procedures used. Some of the papers describe in one or a few steps how two components can be separated by the investigator manipulating their partitions so that one component is in one phase and the other component is in the other phase or at the interface. Investigators can also avail of developed batch extractions for plant organelles, cell membranes, nucleic acids, and proteins. The book cites as an example the partitioning of right-side-out and inside-out vesicles (obtained from fragments of thylakoid membranes) to the top and bottom phases, respectively, of a Dx-PEG system. Other papers describe the use of the countercurrent distribution when single extraction steps are not sufficient to produce a separation in materials that do not differ greatly in their partitioning behavior. The collection can prove valuable for bio-chemists, cellular biologists, micro-biologists, and developmental biologists.
| Author | : Pallippattu Krishnan Vijayan |
| Publisher | : Woodhead Publishing |
| Total Pages | : 651 |
| Release | : 2019-06-19 |
| Genre | : Technology & Engineering |
| ISBN | : 0081024878 |
Single-Phase, Two-Phase and Supercritical Natural Circulation Systems provides readers with a deep understanding of natural circulation systems. This book equips the reader with an understanding on how to detect unstable loops to ensure plant safety and reliability, calculate heat transport capabilities, and design effective natural circulation loops, stability maps and parallel channel systems. Each chapter begins with an introduction to the circulation system before discussing each element in detail and analyzing its effect on the performance of the system. The book also presents thermosyphon heat transport devices in nuclear and other industrial plants, a common information need for students and researchers alike. This book is invaluable for engineers, designers, operators and consultants in nuclear, mechanical, electrical and chemical disciplines. - Presents single-phase, two-phase and supercritical natural circulation systems together in one resource to fill an existing knowledge gap - Guides the reader through relevant processes, such as designing, analyzing and generating stability maps and natural circulation loops, calculating heat transport capabilities, and maintaining natural circulation system operations - Includes global case studies and examples to increase understanding, along with important IAEA standards and procedures
| Author | : Marco Rito-Palomares |
| Publisher | : Springer |
| Total Pages | : 196 |
| Release | : 2017-07-18 |
| Genre | : Technology & Engineering |
| ISBN | : 3319593099 |
This comprehensive and unique text presents a full overview of downstream processing useful for those new to the concept as well as professionals with experience in the area. The history and theoretical principles of Aqueous Two-Phase Systems (ATPS) are covered in depth. Information on ATPS characterization and application is included, and ATPS equilibria and system parameters that have significant effect on partition behavior are studied. Aqueous Two-Phase Systems for Bioprocess Development for the Recovery of Biological Products addresses specific applications of ATPS for the recovery and partial purification of high molecular weight compounds such as proteins, nucleic acids and polysaccharides, particulate bioproducts such as cells and organelles and low molecular weight compounds. Non-conventional strategies involving ATPS such as affinity systems, continuous liquid-liquid fractionation stages and the recovery from plant extracts are presented. Economic analysis of the application of ATPS in comparison to other fractionation techniques, particularly liquid chromatography, is considered, as are opportunity and current trends in the ATPS research area. Each chapter utilizes the contributors' experimental expertise in traditional and non-conventional ATPS strategies, as well as analysis of areas of opportunity and perspectives on the development and future applications of ATPS in both the lab and larger scale operations. The result is a thorough and singular overview of ATPS which has not been matched by any other text on the market.
| Author | : Salomon Levy |
| Publisher | : John Wiley & Sons |
| Total Pages | : 450 |
| Release | : 1999-08-02 |
| Genre | : Technology & Engineering |
| ISBN | : 9780471329671 |
Mehrphasige Strömungen spielen in etlichen Industriezweigen, besonders der Luft- und Raumfahrt und der Energieerzeugung, eine zentrale Rolle. Derart komplexe Strömungsvorgänge sind extrem schwer vorauszuberechnen, zu analysieren und zu testen. Wertvolle Hilfestellungen, die für reale technische Situationen gedacht sind, gibt dieser Band, der auch Quelltexte einschlägiger Computerprogramme enthält. (07/99)
| Author | : Vangelis Anatolijs Xanthopoulos |
| Publisher | : |
| Total Pages | : 0 |
| Release | : 2018 |
| Genre | : Phase partition |
| ISBN | : 9781536142419 |
Aqueous Two-Phase Systems (ATPS) have been used since their discovery for the isolation of a large number of different biological materials, such as animal and plant cells, microorganisms, viruses, cellular organelles such as chloroplasts, mitochondria, membrane vesicles, and also in the purification of proteins and nucleic acids. In Aqueous Two-Phase Systems: Properties, Functions and Advantages, the authors begin by discussing the benefits of these systems.The following chapter presents the main forms of the purification of lipases from aqueous biphasic systems, addressing and discussing the fundamentals for the formation, use and optimization of these systems applied in the biotechnological context. The authors address the basis of aqueous-biphasic systems, aqueous two-phase systems for enzymatic purification, purification of lipases and combined uses of aqueous two-phase systems.The most important experimental parameters that affect the efficiency of metal ions extraction in aqueous PEG-based two-phase systems are examined to provide a starting point in the design of a suitable system for the extraction of metal ions. Compared to the conventional solvent extraction, which uses toxic, flammable and volatile organic solvents and can be quite expensive, the extraction in aqueous PEG-based two-phase systems is considered a more environmental friendly and economically viable method.Also in this compilation, an overview about application of ATPSs in microstructured devices is provided. Microstructured devices offer potential benefits due to well-defined high specific interfacial areas available for heat and mass transfer. These areas increase transfer rate and enhance yield, selectivity and process control. The application of ATPSs in Micro Total Analysis Systems is described as well.
| Author | : Boris Y. Zaslavsky |
| Publisher | : CRC Press |
| Total Pages | : 720 |
| Release | : 1994-11-15 |
| Genre | : Science |
| ISBN | : 9780824794613 |
Covers the fundamental principles of solute partitioning in aqueous two-phase systems, explains their important practical features, and furnishes methods of characterization. The information provided by the partition behaviour of a solute in an aqueous two-phase system is examined.
| Author | : Mamoru Ishii |
| Publisher | : Springer Science & Business Media |
| Total Pages | : 462 |
| Release | : 2006-09-28 |
| Genre | : Technology & Engineering |
| ISBN | : 0387291873 |
This book has been written for graduate students, scientists and engineers who need in-depth theoretical foundations to solve two-phase problems in various technological systems. Based on extensive research experiences focused on the fundamental physics of two-phase flow, the authors present the detailed theoretical foundation of multi-phase flow thermo-fluid dynamics as they apply to a variety of scenarios, including nuclear reactor transient and accident analysis, energy systems, power generation systems and even space propulsion.
| Author | : Harry Walter |
| Publisher | : Elsevier |
| Total Pages | : 768 |
| Release | : 1994-04-18 |
| Genre | : Nature |
| ISBN | : 9780121821296 |
General methodology and apparatus: phase diagrams, preparation and analysis of two-phase systems, partioning and affinity partitioning of macromolecules: Proteins, nucleic acids, studies on protein interactionsmolecular structure, charge, hydrophobicity, and conformational chan ges, partitioning and affinity partitioning of particulates,organellesseparation and subfractionation, menbrane: separation and subfractionation, membrane domain analysis, aqueous phase separation in biologicalsystems, aqueous two-phase systems in large-scale process biotechnolo gy, proteins; downstream processing, design of proteins for enhanced extraction, other applications of aqueous phases in biotechnology. Enzymology.
| Author | : Sümer M. Peker |
| Publisher | : Elsevier |
| Total Pages | : 535 |
| Release | : 2011-04-18 |
| Genre | : Science |
| ISBN | : 0080553419 |
This book is an undertaking of a pioneering work of uniting three vast fields of interfacial phenomena, rheology and fluid mechanics within the framework of solid-liquid two phase flow. No wonder, much finer books will be written in the future as the visionary aims of many nations in combining molecular chemistry, biology, transport and interfacial phenomena for the fundamental understanding of processes and capabilities of new materials will be achieved. Solid-liquid systems where solid particles with a wide range of physical properties, sizes ranging from nano- to macro- scale and concentrations varying from very dilute to highly concentrated, are suspended in liquids of different rheological behavior flowing in various regimes are taken up in this book. Interactions among solid particles in molecular scale are extended to aggregations in the macro scale and related to settling, flow and rheological behavior of the suspensions in a coherent, sequential manner. The classical concept of solid particles is extended to include nanoparticles, colloids, microorganisms and cellular materials. The flow of these systems is investigated under pressure, electrical, magnetic and chemical driving forces in channels ranging from macro-scale pipes to micro channels. Complementary separation and mixing processes are also taken under consideration with micro- and macro-scale counterparts.- Up-to-date including emerging technologies- Coherent, sequential approach- Wide scope: microorganisms, nanoparticles, polymer solutions, minerals, wastewater sludge, etc- All flow conditions, settling and non-settling particles, non-Newtonian flow, etc- Processes accompanying conveying in channels, such as sedimentation, separation, mixing
