Transposable Elements
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| Author | : Jungnam Cho |
| Publisher | : Humana |
| Total Pages | : 272 |
| Release | : 2022-05-11 |
| Genre | : Science |
| ISBN | : 9781071611364 |
This volume details the most up-to-date technologies used in plant transposable element studies and provides easy-to-follow protocols. Chapters guide readers on available database resources, annotation of different families of transposon, and experimental methods to detect their transposition intermediates, neo-transposed DNAs, and transposition events. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Plant Transposable Elements: Methods and Protocols aims to provide web-lab and dry-lab methodologies targeted at various levels from beginner to experienced.
| Author | : Jeffrey Bennetzen |
| Publisher | : Springer |
| Total Pages | : 390 |
| Release | : 2018-11-24 |
| Genre | : Science |
| ISBN | : 3319974270 |
This book discusses advances in our understanding of the structure and function of the maize genome since publication of the original B73 reference genome in 2009, and the progress in translating this knowledge into basic biology and trait improvement. Maize is an extremely important crop, providing a large proportion of the world’s human caloric intake and animal feed, and serving as a model species for basic and applied research. The exceptionally high level of genetic diversity within maize presents opportunities and challenges in all aspects of maize genetics, from sequencing and genotyping to linking genotypes to phenotypes. Topics covered in this timely book range from (i) genome sequencing and genotyping techniques, (ii) genome features such as centromeres and epigenetic regulation, (iii) tools and resources available for trait genomics, to (iv) applications of allele mining and genomics-assisted breeding. This book is a valuable resource for researchers and students interested in maize genetics and genomics.
| Author | : Barbara McClintock |
| Publisher | : Routledge |
| Total Pages | : 635 |
| Release | : 1987 |
| Genre | : Religion |
| ISBN | : 9780824013912 |
The introduction was written by Barbara McClintock to show how the concept of transposable elements evolved, and to comment on subsequent investigations of these elements. The papers in this volume were selected because of their relevance to this topic. For the discovery of "Mobile genetic elements" she received the Nobel Prize for Physiology or Medicine in 1983.
| Author | : Douglas E. Berg |
| Publisher | : Amer Society for Microbiology |
| Total Pages | : 972 |
| Release | : 1989-01 |
| Genre | : Medical |
| ISBN | : 9781555810054 |
Documents the remarkable mobility of DNA in procaryotic and eucaryotic genomes: the ability of various DNA segments to move to new sites, to invert, and to undergo deletion or amplification, generally without the extensive DNA sequence homology needed for classical recombination. Seventy contributors explore the mechanisms of these rearrangements, how they are regulated, their biological consequences, and their potential use as research tools. For students and researchers of molecular genetics. Annotation copyrighted by Book News, Inc., Portland, OR
| Author | : Michael Chandler |
| Publisher | : John Wiley & Sons |
| Total Pages | : 1321 |
| Release | : 2020-07-24 |
| Genre | : Science |
| ISBN | : 1555819214 |
An exploration of the raw power of genetic material to refashion itself to any purpose... Virtually all organisms contain multiple mobile DNAs that can move from place to place, and in some organisms, mobile DNA elements make up a significant portion of the genome. Mobile DNA III provides a comprehensive review of recent research, including findings suggesting the important role that mobile elements play in genome evolution and stability. Editor-in-Chief Nancy L. Craig assembled a team of multidisciplinary experts to develop this cutting-edge resource that covers the specific molecular mechanisms involved in recombination, including a detailed structural analysis of the enzymes responsible presents a detailed account of the many different recombination systems that can rearrange genomes examines the tremendous impact of mobile DNA in virtually all organisms Mobile DNA III is valuable as an in-depth supplemental reading for upper level life sciences students and as a reference for investigators exploring new biological systems. Biomedical researchers will find documentation of recent advances in understanding immune-antigen conflict between host and pathogen. It introduces biotechnicians to amazing tools for in vivo control of designer DNAs. It allows specialists to pick and choose advanced reviews of specific elements and to be drawn in by unexpected parallels and contrasts among the elements in diverse organisms. Mobile DNA III provides the most lucid reviews of these complex topics available anywhere.
| Author | : Esra Galun |
| Publisher | : Springer Science & Business Media |
| Total Pages | : 342 |
| Release | : 2013-04-17 |
| Genre | : Nature |
| ISBN | : 9401735824 |
OY (])PONEOYLI TOIA YTA IIOAAOI OKOEOJII Many fail to grasp what they have seen, and cannot judge what they have learned, ErKYPEOYLI OYL1E MA®ONTEE ITINOEKOYIT although they tell themselves they know. EQYTOJII L1E L10KEOYLI Heraclitus of Ephesus, 500 BC " ... everyone that is not speckled and C~T~v: N,;~, 'T,ji~ ,~~~N ,tuN '= spotted among the goats and brown among the sheep, that shall be counted ~~N N,n =,~, c~w=== c,m stolen with me." Genesis Chapter 30 From Heraclitus of Ephesus and later philosophers, we can deduce that observation of natural phenomena, even when keen and accurate, will not result in meaningful knowledge unless combined with analysis of the mind; just as analysis of the mind without acquaintance with natural phenomena will not suffice to grasp the perceivable world. Only familiarity with phenomena combined with mental analysis will lead to additional knowledge. The citation from Genesis, Chapter 30, is part of an unusual story. It tells how Jacob received, as payment for his service to Laban, the bulk of Laban's herds. By agreement, Jacob was to receive "only" the newborn speckled and spotted goats and the newborn brown sheep that differed completely from their parents. Did Jacob know that there was instability (transposable elements?) in the pigmentation of Laban's herd? It is reasonable to assume that Jacob combined his keen observation with analysis of his mind in order to predict the outcome: most of the newborns were indeed speckled, spotted or brown.
| Author | : Jose Luis Garcia Perez |
| Publisher | : Humana |
| Total Pages | : 0 |
| Release | : 2016-02-20 |
| Genre | : Medical |
| ISBN | : 9781493933709 |
This volume covers the latest protocols designed to identify and characterize TEs in genomes, ancient or recently inserted. Additionally, this book includes a series of protocols designed to understand how host genomes act to regulate the activity of TEs, from elegant genetic mobilization assays to key biochemical methods. Finally, this book also includes chapters that describe how TEs can be used for biotechnological applications. Written for the Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Transposons and Retrotransposons: Methods and Protocols aims to ensure successful results in the further study of this vital field.
| Author | : O. Nelson |
| Publisher | : Springer Science & Business Media |
| Total Pages | : 396 |
| Release | : 2012-12-06 |
| Genre | : Medical |
| ISBN | : 1468455508 |
Transposon tagging can work. Even though most of our understand ing about the factors that contribute to a successful tagging experiment has been accumulated from a limited number of experiments using different transposable elements in different genetic backgrounds, it is still possible to draw some conclusions regarding the best experimental strategies for gene tagging. In our experience, Spm has proved to be a good element for transposon tagging. The frequency of recovering mutable alleles in duced by Spm is not significantly different from that for Ac-Ds or for Mu 6 (summarized in Ref. 22) and varies from about 10- to 10=zr:-8pm has the unique advantage, however, in that all of the members of thiSfumily that have been examined thus far are homologous to each other at the DNA level. Therefore, by combining molecular analysis with genetic segre gation, it is possible to identify and isolate alleles that are due to insertions of either autonomous or nonautonomous Spm elements. There are definite steps one can take to increase the chances of de tecting a transposition into the gene of interest. The most important step is to select a genetic background in which the desired phenotype will be easy to screen. If the phenotype is not likely to be mutable, then tester lines should be constructed so as to contain flanking markers that can aid in subsequent segregation analyses.
| Author | : Nancy L. Craig |
| Publisher | : Amer Society for Microbiology |
| Total Pages | : 1204 |
| Release | : 2002 |
| Genre | : Science |
| ISBN | : 9781555812096 |
An extension of the original volume, reflecting the latest advances in understanding these elements. This title is published by the American Society for Microbiology Press and distributed by Taylor and Francis in rest of world territories.
| Author | : Andrew G. Clark |
| Publisher | : MDPI |
| Total Pages | : 206 |
| Release | : 2020-03-05 |
| Genre | : Science |
| ISBN | : 3039283669 |
Repetitive DNA is ubiquitous in eukaryotic genomes, and, in many species, comprises the bulk of the genome. Repeats include transposable elements that can self-mobilize and disperse around the genome, and tandemly-repeated satellite DNAs that increase in copy number due to replication slippage and unequal crossing over. Despite their abundance, repetitive DNA is often ignored in genomic studies due to technical challenges in their identification, assembly, and quantification. New technologies and methods are now providing the unprecedented power to analyze repetitive DNAs across diverse taxa. Repetitive DNA is of particular interest because it can represent distinct modes of genome evolution. Some repetitive DNA forms essential genome structures, such as telomeres and centromeres, which are required for proper chromosome maintenance and segregation, whereas others form piRNA clusters that regulate transposable elements; thus, these elements are expected to evolve under purifying selection. In contrast, other repeats evolve selfishly and produce genetic conflicts with their host species that drive adaptive evolution of host defense systems. However, the majority of repeats likely accumulate in eukaryotes in the absence of selection due to mechanisms of transposition and unequal crossing over. Even these neutral repeats may indirectly influence genome evolution as they reach high abundance. In this Special Issue, the contributing authors explore these questions from a range of perspectives.
