Transcriptome Sequencing in Pigeonpea [Cajanus Cajan (L.) Millsp.]

Transcriptome Sequencing in Pigeonpea [Cajanus Cajan (L.) Millsp.]
Author: Sutapa Dutta
Publisher: LAP Lambert Academic Publishing
Total Pages: 188
Release: 2013
Genre:
ISBN: 9783659458279

Genomics research has great potential to revolutionize the discipline of plant breeding. Pigeonpea is an important grain legume mainly produced in semi-arid region, where it plays a crucial role in food and nutritional security of the people. It has the potential to be identified as an important food crop under changing climate. Pedigree and molecular diversity analysis in cultivars developed through classical breeding approaches indicated narrow genetic base in pigeonpea. Genetic and genomic improvement of pigeonpea is required to increase the productivity to meet the increasing food demand. Areas covered in this book include, construction and preparation for Expressed Sequence Tags library, sequencing by most traditional as well as next generation platform, assembling sequencing data, gene prediction, development of molecular markers (SSRs) and evaluation of this bio resources in biodiversity, phylogeny analysis across legume species. The book, therefore, should prove useful not only for students and teachers, but also for the young researchers starting their research career in the field of plant genomics.

The Pigeonpea Genome

The Pigeonpea Genome
Author: Rajeev K. Varshney
Publisher: Springer
Total Pages: 113
Release: 2017-12-15
Genre: Science
ISBN: 3319637975

Pigeonpea (Cajanus cajan) is a crop of small land holding farmers in arid and semi-arid regions of the world. It has a number of usages starting from protein rich food to vegetarian families; fuel wood; nitrogen supplier to soil; recycling minerals in soil to animal feed etc. Pigeonpea has been considered to be originated and domesticated in central India from where it travelled to different parts of the world such as Africa and Latin America. In ongoing scenario of climate change, biotic and especially abiotic stresses will make the conditions more challenging for entire agriculture. This volume focusing on the pigeonpea genome will collate the information on the genome sequencing and its utilization in genomics activities, with a focus on the current findings, advanced tools and strategies deployed in pigeonpea genome sequencing and analysis, and how this information is leading to direct outcomes for plant breeders and subsequently to farmers.

Genetic and Genomic Resources of Grain Legume Improvement

Genetic and Genomic Resources of Grain Legume Improvement
Author: Hari D. Upadhyaya
Publisher: Elsevier Inc. Chapters
Total Pages: 34
Release: 2013-07-18
Genre: Science
ISBN: 0128064420

Pigeon pea (Cajanus cajan (L.) Millspaugh) is an important grain legume crop grown in tropical and subtropical regions of the world. Though pigeon pea has a narrow genetic base, vast genetic resources are available for its genetic improvement. Evaluation of small subsets, such as core (10% of the whole collection) and mini-core collections (about 1% of the entire collection), has resulted in the identification of promising diverse sources for agronomic and nutrition-related traits as well as resistance/tolerance to important biotic/abiotic stresses for use in pigeon pea improvement programmes. Wild relatives of pigeon pea are the reservoir of many useful genes, including resistance/tolerance to diseases, insect pests and drought and good agronomic traits, and also have contributed to the development of cytoplasmic male sterility systems for pigeon pea improvement. Availability of genomic resources, including the genome sequence, will facilitate greater use of germplasm to develop new cultivars with a broad genetic base.

Genotyping by Sequencing for Crop Improvement

Genotyping by Sequencing for Crop Improvement
Author: Humira Sonah
Publisher: John Wiley & Sons
Total Pages: 404
Release: 2022-03-29
Genre: Science
ISBN: 1119745675

OGENOTYPING BY SEQUENCING FOR CROP IMPROVEMENT A thoroughly up-to-date exploration of genotyping-by-sequencing technologies and related methods in plant science In Genotyping by Sequencing for Crop Improvement, a team of distinguished researchers delivers an in-depth and current exploration of the latest advances in genotyping-by-sequencing (GBS) methods, the statistical approaches used to analyze GBS data, and its applications, including quantitative trait loci (QTL) mapping, genome-wide association studies (GWAS), and genomic selection (GS) in crop improvement. This edited volume includes insightful contributions on a variety of relevant topics, like advanced molecular markers, high-throughput genotyping platforms, whole genome resequencing, QTL mapping with advanced mapping populations, analytical pipelines for GBS analysis, and more. The distinguished contributors explore traditional and advanced markers used in plant genotyping in extensive detail, and advanced genotyping platforms that cater to unique research purposes are discussed, as is the whole-genome resequencing (WGR) methodology. The included chapters also examine the applications of these technologies in several different crop categories, including cereals, pulses, oilseeds, and commercial crops. Genotyping by Sequencing for Crop Improvement also offers: A thorough introduction to molecular marker techniques and recent advancements in the technology Comprehensive explorations of the genotyping of seeds while preserving their viability, as well as advances in genomic selection Practical discussions of opportunities and challenges relating to high throughput genotyping in polyploid crops In-depth examinations of recent advances and applications of GBS, GWAS, and GS in cereals, pulses, oilseeds, millets, and commercial crops Perfect for practicing plant scientists with an interest in genotyping-by-sequencing technology, Genotyping by Sequencing for Crop Improvement will also earn a place in the libraries of researchers and students seeking a one-stop reference on the foundational aspects of – and recent advances in – genotyping-by-sequencing, genome-wide association studies, and genomic selection.

Smart Plant Breeding for Field Crops in Post-genomics Era

Smart Plant Breeding for Field Crops in Post-genomics Era
Author: Devender Sharma
Publisher: Springer Nature
Total Pages: 424
Release: 2023-04-28
Genre: Science
ISBN: 981198218X

This book emphasizes on cutting-edge next-generation smart plant breeding approaches for maximizing the use of genomic resources generated by high-throughput genomics in the post-genomic era. Through this book the readers would learn about the recent development in the genomic approaches such as genotype by sequencing (GBS) for genomic analysis (SNPs, Single Nucleotide Polymorphism), whole-genome re-sequencing (WGRS) and RNAseq for transcriptomic analysis (DEGs, Differentially Expressed Genes). To maximize the genetic gains in the cereal/food crops, the book covers topics on transgenic breeding, genome editing, high-throughput phenotyping, reliable/precision phenotyping and genomic information-based analysis. In the era of climate change and the ever-increasing population, food security and nutritional security are the primary concern of plant breeders, growers, and policymakers to address the UN’s sustainable development goals. Chapters of this book cohere around these goals and covers techniques such as (QTL mapping, association studies, candidate gene identification), omics, RNAi [through micro RNA (miRNA), small interfering RNA (siRNA) and artificial micro RNA (amiRNA)]. It also covers other genomic techniques like antisense technology, genome editing (CRISPR/cas9, base editing) and epigenomics that assist the crop improvement programmes to fulfil the UNs sustainable development goals. It explores the influence of rapidly available sequencing data assisting in the next generation breeding programmes. This volume is a productive resource for the students, researchers, scientists, teachers, public and private sector stakeholders involved in the genetic enhancement of cereal crops.

Underutilised Crop Genomes

Underutilised Crop Genomes
Author: Mark A. Chapman
Publisher: Springer Nature
Total Pages: 460
Release: 2022-11-03
Genre: Science
ISBN: 3031008480

This book highlights the uses for underutilized crops, presenting the state-of-the-art in terms of genome sequencing for over 30 crops, previously understudied and under-researched. In a changing climate and with significant pressure on the land, it is the ideal time to be discussing novel crops, with significant biotic and abiotic tolerances and/or rich nutrient profiles for consumers. Previously, the only species with sequenced genomes were high-profile internationally recognized crops, but in the current era genomes are being sequenced for dozens of crops, including those previously classified as underutilized, now being investigated. This book covers food crops, from fruits to tubers, and from grasses to legumes, as well as crops with non-food applications. Some of these crops have draft genomes, and others have polished genomes with extensive resequencing panels. Each chapter tells the story of an individual crop or crop group, written by experts, focusing on the genome data available, revealing more about crop domestication and genetic variation, and the current and future prospects given that this data is now becoming available. It also highlights how even small sequencing projects can provide draft genome sequences suitable for gene discovery, comparative genomics, and identification of molecular markers for understanding these crops further.

Genetic and Genomic Resources of Grain Legume Improvement

Genetic and Genomic Resources of Grain Legume Improvement
Author: Mohar Singh
Publisher: Newnes
Total Pages: 322
Release: 2013-07-18
Genre: Science
ISBN: 0123984947

Grain legumes, including common-bean, chickpea, pigeonpea, pea, cowpea, lentil and others, form important constituents of global diets, both vegetarian and non-vegetarian. Despite this significant role, global production has increased only marginally in the past 50 years. The slow production growth, along with a rising human population and improved buying capacity has substantially reduced the per capita availability of food legumes. Changes in environmental climate have also had significant impact on production, creating a need to identify stable donors among genetic resources for environmentally robust genes and designing crops resilient to climate change. Genetic and Genomic Resources of Grain Legume Improvement is the first book to bring together the latest resources in plant genetics and genomics to facilitate the identification of specific germplasm, trait mapping and allele mining to more effectively develop biotic and abiotic-stress-resistant grains. This book will be an invaluable resource for researchers, crop biologists and students working with crop development. - Explores origin, distribution and diversity of grain legumes - Presents information on germplasm collection, evaluation and maintenance - Offers insight into pre-breeding/germplasm enhancement efforts - Integrates genomic and genetic resources in crop improvement - Internationally contributed work