Three Dimensional And Multidimensional Microscopy
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Multidimensional Microscopy
| Author | : P.-C. Cheng |
| Publisher | : Springer Science & Business Media |
| Total Pages | : 394 |
| Release | : 2012-12-06 |
| Genre | : Science |
| ISBN | : 1461383668 |
Modern cell biology is being revolutionized by the wedding of microscopy and computers. This book describes the new instrumentation and methods which allow three-dimensional reconstruction of specimens. Multidimensional Microscopy will be of interest to cell biologists, microscopists, and basic biomedical researchers whose work involves microscopic techniques. This book presents current results on a very active field in modern biology: methods in light and electron microscopy that allow the reconstruction of three-dimensional objects with the aid of computers. The book emphasizes the methods that can be used and examples of biological systems to which they have been applied. It includes extensive descriptions of confocal microscopy and its applications, as well as chapters on X-ray microscopy, low-voltage electron microscopy, and image reconstruction. This is an impressive summary of state-of-the-art methods in microscopy, in which microscopes and computers are being joined to permit specimens to be examined and reconstructed in three dimensions. Will be of interest to cell biologists, biomedical researchers, and microscopists.
Focus on Multidimensional Microscopy
| Author | : Ping-chin Cheng |
| Publisher | : World Scientific |
| Total Pages | : 326 |
| Release | : 2000-01-01 |
| Genre | : Confocal microscopy |
| ISBN | : 9789810230531 |
This book covers various aspects of modern microscopy, with emphasis on multidimensional (three-dimensional and higher) and multimodality microscopy. The topics discussed include multiphoton fluorescent microscopy, confocal microscopy, x-ray microscopy and microtomography, electron microscopy, probe microscopy and multidimensional image processing for microscopy. In addition, there are chapters demonstrating typical microscopical applications, both biological and material
Unconventional Optical Imaging for Biology
| Author | : Corinne Fournier |
| Publisher | : John Wiley & Sons |
| Total Pages | : 308 |
| Release | : 2024-05-21 |
| Genre | : Medical |
| ISBN | : 1789451329 |
Optical imaging of biological systems has undergone spectacular development in recent years, producing a quantity and a quality of information that, just twenty years ago, could only be dreamed of by physicists, biologists and physicians. Unconventional imaging systems provide access to physical quantities – phase, absorption, optical index, the polarization property of a wave or the chemical composition of an object – not accessible to conventional measurement systems. To achieve this, these systems use special optical setups and specific digital image processing to reconstruct physical quantities. This field is also known as computational imaging. This book presents various non-conventional imaging modalities developed for the biomedical field: wave front analysis imaging, digital holography/tomography, optical nanoscopy, endoscopy and singlesensor imaging. Experimental setups and reconstruction algorithms are presented for each modality.
Focus On Multidimensional Microscopy (In 2 Vols) - Volume 2
| Author | : Ping-chin Cheng |
| Publisher | : World Scientific |
| Total Pages | : 338 |
| Release | : 1999-12-28 |
| Genre | : Science |
| ISBN | : 981449772X |
This book covers various aspects of modern microscopy, with emphasis on multidimensional (three-dimensional and higher) and multimodality microscopy. The topics discussed include multiphoton fluorescent microscopy, confocal microscopy, x-ray microscopy and microtomography, electron microscopy, probe microscopy and multidimensional image processing for microscopy. In addition, there are chapters demonstrating typical microscopical applications, both biological and material.
Three-dimensional Electron Microscopy of Macromolecular Assemblies
| Author | : Joachim Frank |
| Publisher | : Oxford University Press |
| Total Pages | : 427 |
| Release | : 2006 |
| Genre | : Computers |
| ISBN | : 0195150961 |
Cryoelectron microscopy of biological molecules is among the hottest growth areas in biophysics and structural biology at present, and Frank is arguably the most distinguished practitioner of this art. CryoEM is likely over the next few years to take over much of the structural approaches currently requiring X-ray crystallography, because one can now get good and finely detailed images of single molecules down to as little as 200,000 MW, covering a substantial share of the molecules of greatest biomedical research interest. This book, the successor to an earlier work published in 1996 with Academic Press, is a natural companion work to our forthcoming book on electron crystallography by Robert Glaeser, with contributions by six others, including Frank. A growing number of workers will employ CryoEM for structural studies in their own research, and a large proportion of biomedical researchers will have a growing interest in understanding what the capabilities and limits of this approach are.
Handbook of Biological Confocal Microscopy
| Author | : James Pawley |
| Publisher | : Springer Science & Business Media |
| Total Pages | : 639 |
| Release | : 2013-04-17 |
| Genre | : Science |
| ISBN | : 1475753489 |
This third edition of a classic text in biological microscopy includes detailed descriptions and in-depth comparisons of parts of the microscope itself, digital aspects of data acquisition and properties of fluorescent dyes, the techniques of 3D specimen preparation and the fundamental limitations, and practical complexities of quantitative confocal fluorescence imaging. Coverage includes practical multiphoton, photodamage and phototoxicity, 3D FRET, 3D microscopy correlated with micro-MNR, CARS, second and third harmonic signals, ion imaging in 3D, scanning RAMAN, plant specimens, practical 3D microscopy and correlated optical tomography.
Focus On Multidimensional Microscopy (In 2 Vols) - Volume 1
| Author | : Ping-chin Cheng |
| Publisher | : World Scientific |
| Total Pages | : 320 |
| Release | : 1999-12-28 |
| Genre | : Science |
| ISBN | : 9814497711 |
This book covers various aspects of modern microscopy, with emphasis on multidimensional (three-dimensional and higher) and multimodality microscopy. The topics discussed include multiphoton fluorescent microscopy, confocal microscopy, x-ray microscopy and microtomography, electron microscopy, probe microscopy and multidimensional image processing for microscopy. In addition, there are chapters demonstrating typical microscopical applications, both biological and material.
Cell Biological Applications of Confocal Microscopy
| Author | : |
| Publisher | : Elsevier |
| Total Pages | : 521 |
| Release | : 2003-01-04 |
| Genre | : Science |
| ISBN | : 008049658X |
This volume of the acclaimed Methods in Cell Biology series provides specific examples of applications of confocal microscopy to cell biological problems. It is an essential guide for students and scientists in cell biology, neuroscience, and many other areas of biological and biomedical research, as well as research directors and technical staff of microscopy and imaging facilities. An integrated and up-to-date coverage on the many various techniques and uses of the confocal microscope (CM). - Includes detailed protocols accessible to new users - Details how to set up and run a "Confocal Microscope Core Facility" - Contains over 170 figures
MicroComputed Tomography
| Author | : Stuart R. Stock |
| Publisher | : CRC Press |
| Total Pages | : 390 |
| Release | : 2019-12-06 |
| Genre | : Technology & Engineering |
| ISBN | : 1498775012 |
MicroComputed Tomography has become the gold standard for studying 3D microscopic structures nondestructively, and this book provides up-to-date coverage of the modality. The first part of the book focuses on methodology, covering experimental methods, data analysis, and visualization approaches. Emphasis is on fundamentals so that those new to the field can design their own effective microCT studies. The second part addresses various microCT applications, organized by type of microstructure so that the reader can appreciate approaches from other disciplines. The applications include porous solids, microstructural evolution, soft tissue studies, applications using x-ray phase contrast or x-ray scattering contrast, and multimode studies.
