Thidiazuron: From Urea Derivative to Plant Growth Regulator

Thidiazuron: From Urea Derivative to Plant Growth Regulator
Author: Naseem Ahmad
Publisher: Springer
Total Pages: 489
Release: 2018-03-23
Genre: Science
ISBN: 9811080046

Plant biotechnology is a most interesting branch for academicians and researchers in recent past. Now days, it becomes a very useful tool in agriculture and medicine and is regarded as a popular area of research especially in biological sciences because it makes an integral use of biochemistry, molecular biology and engineering sciences in order to achieve technological application of cultured tissues, cell and microbes. Plant tissue culture (PTC) refers to a technique of cultivation of plant cells and other parts on artificial nutrient medium in controlled environment under aseptic conditions. PTC requires various nutrients, pH, carbon source, gelling agent, temperature, photoperiod, humidity etc. and most importantly the judicious use of plant growth regulators. Various natural, adenine and phenyl urea derivatives are employed for the induction and proliferation of different types of explants. Several phenyl urea derivatives were evaluated and it was observed that thidiazuron (n-phenyl-N”-1,2,3- thidiazol-5-ulurea) was found to be the most active among the plant growth regulators. Thidiazuron (TDZ) was initially developed as a cotton defoliant and showed high cytokinin like activity. In some examples, its activity was 100 times more than BA in tobacco callus assay and produces more number of shoots in cultures than Zeatin and 2iP. TDZ also showed major breakthrough in tissue culture of various recalcitrant legumes and woody species. For the last two decades, number of laboratories has been working on TDZ with different aspect and number of publications has come out. To the best of our knowledge, there is no comprehensive edited volume on this particular topic. Hence th,e edited volume is a deed to consolidate the scattered information on role of TDZ in plant tissue culture and genetic manipulations that would hopefully prove informative to various researches. Thidiazuron: From Urea Derivative to Plant Growth Regulator compiles various aspects of TDZ in Plant Tissue Culture with profitable implications. The book will provides basic material for academicians and researchers who want to initiate work in this fascinating area of research. The book will contain 26 chapters compiled by International dignitaries and thus giving a holistic view to the edited volume.

Meta-topolin: A Growth Regulator for Plant Biotechnology and Agriculture

Meta-topolin: A Growth Regulator for Plant Biotechnology and Agriculture
Author: Naseem Ahmad
Publisher: Springer Nature
Total Pages: 339
Release: 2021-05-02
Genre: Science
ISBN: 981159046X

Plant tissue culture (PTC) technology has gained unassailable success for its various commercial and research applications in plant sciences. Plant growth regulators (PGRs) are an essential part of any plant tissue culture intervention for propagation or modification of plants. A wide range of PGRs are available, including aromatic compounds that show cytokinin activities, promote cell division and micro-propagation, viz. kinetin, N6-benzyladenine and topolins. Topolins are naturally occurring aromatic compounds that have gained popularity as an effective alternative for other frequently used cytokinins in in vitro culture of plants. Among them, meta-topolin [6-(3-hydroxybenzlyamino) purine] is the most popular and its use in plant tissue culture has amplified swiftly. During the last few decades, there have been numerous reports highlighting the effectiveness of meta-topolin in micropropagation and alleviation of various physiological disorders, rooting and acclimatization of tissue culture raised plants.

Micropropagation of Medicinal Plants: Volume 2

Micropropagation of Medicinal Plants: Volume 2
Author: T. Pullaiah
Publisher: Bentham Science Publishers
Total Pages: 391
Release: 2024-03-29
Genre: Science
ISBN: 9815238310

This volume presents information about protocols for micropropagation of more than 40 species of medicinal plants. The contents combine knowledge about the scientific principles of micropropagation with state of the art updates in tissue culture techniques presented by plant scientists. The readers will learn about techniques required to grow plants in challenging conditions that aim to reduce the impacts of injudicious harvesting, deforestation, climate change, pollution, urbanization and other factors that limit the ability to meet current demand. General topics such as biotization and pharmaceutical investigation are also included to guide readers about the significance of these plants in research and development for new medicines. The book provides protocols for micropropagation of important medicinal plants like Rauvolfia serpentina, Catharanthus roseus, Withania somnifera, Tylophora indica, Bacopa monnieri, Aloe vera, Phyllanthus amarus, Allium sativum, Moringa oleifera, Operculina turpethum, Glycyrrhiza glabra, Pterocarpus marsupium, Vetiver grass, Ruta graveolens, Tinospora cordifolia, Kaempferia, Hedychium, Decalepis hamiltonii, Saraca asoca, Wrightia tinctoria, Wrightia arborea, Artemisia absinthium, Aegle marmelos, Atropa acuminata, Atropa belladonna, Alpinia species, Hedychium species, and Cissus species. This book is a handy reference for medicinal chemists, horticulturists and pharmacists who want to learn about the growth and conservation of important medicinal herbs and plants.

Micropropagation of Woody Plants

Micropropagation of Woody Plants
Author: M.R. Ahuja
Publisher: Springer Science & Business Media
Total Pages: 540
Release: 1992-11-30
Genre: Science
ISBN: 9780792318071

This volume covers recent advances in the vegetative propagation of woody plants by tissue culture. A wide range of topics relevant to micropropagation of woody plants are discussed by renowned international scientists. These include cellular contro of morphogenesis, light regimes in tissue culture, maturation and rejuvenation, synthetic seed, genetics of micropropagated plants, haploid embryogenesis, protoplast culture, and acclimatization of ex vitro woody plants. In addition to micropropagation of selected woody plants, both gymnosperms and angiosperms, this volume also includes in vitro genetic selection, strategic planning for application of biotechnology for genetics and breeding, and clonal options for woody plant improvement. A balanced view of both perspectives and limitations of woody plant micropropagation is presented.

Orchid Propagation

Orchid Propagation
Author: Edward Chee-Tak Yeung
Publisher: Springer Nature
Total Pages: 410
Release:
Genre:
ISBN: 1071640313

Commercial Scale Tissue Culture for Horticulture and Plantation Crops

Commercial Scale Tissue Culture for Horticulture and Plantation Crops
Author: Shubhpriya Gupta
Publisher: Springer Nature
Total Pages: 338
Release: 2022-06-16
Genre: Science
ISBN: 9811900558

This edited book is focusing on the novel and innovative procedures in tissue culture for large scale production of plantation and horticulture crops. It is bringing out a comprehensive collection of information on commercial scale tissue culture with the objective of producing high quality, disease-free and uniform planting material. Developing low cost commercial tissue culture can be one of the best possible way to attain the goal of sustainable agriculture. Tissue culture provides a means for rapid clonal propagation of desired cultivars, and a mechanism for somatic hybridization and in vitro selection of novel genotypes. Application of plant tissue culture technology in horticulture and plantation crops provides an efficient method to improve the quality and nutrition of the crops. This book includes a description of highly efficient, low cost in vitro regeneration protocols of important plantation and horticulture crops with a detailed guideline to establish a commercial plant tissue culture facility including certification, packaging and transportation of plantlets. The book discusses somatic embryogenesis, virus elimination, genetic transformation, protoplast fusion, haploid production, coculture of endophytic fungi, effects of light and ionizing radiation as well as the application of bioreactors. This book is useful for a wide range of readers such as, academicians, students, research scientists, horticulturists, agriculturists, industrial entrepreneurs, and agro-industry employees.

Growth Regulators and Biostimulants: Upcoming Opportunities

Growth Regulators and Biostimulants: Upcoming Opportunities
Author: Paromik Bhattacharyya
Publisher: Frontiers Media SA
Total Pages: 287
Release: 2023-10-20
Genre: Science
ISBN: 2832526780

In the recent past, significant strides have been made in the domain of plant growth regulators (PGRs) and biostimulants. In the sustainable utilization of plant germplasm PGRs and biostimulants play a pivotal role. With a magnified growth rate and less risk of inducing clonal somaclonal variations, PGRs (such as auxins, gibberellins, isoprenoid and aromatic cytokinins, ethylene, and abscisic acid) serve as a boon to plant biologists especially those working with rare endangered and threatened species and medicinal and aromatic plant species. Furthermore, the combined effect of PGRs with LEDs (light-emitting diode) on various aspects of plant development is an area of research gaining attention. The use of biostimulants to promote plant growth, yield and stress tolerance has increasingly gained attention. However, their functional role in the growth and development of plants is not clearly understood.

Cytokinins

Cytokinins
Author: David W. S. Mok
Publisher: CRC Press
Total Pages: 353
Release: 2019-07-23
Genre: Medical
ISBN: 1351079735

Cytokinins are hormones involved in all aspects of plant growth and development and are essential for in vitro manipulation of plant cells and tissues. Much information has been gathered regarding the chemistry and biology of cytokinins, while recent studies have focused on the genetics and cytokinin-related genes. However, other than proceedings of symposia, no single volume on cytokinins has been written. This book is the first of its kind, homing in on the key subject areas of cytokinin-chemistry, biosynthesis, metabolism, activity, function, genetics, and analyses. These areas are comprehensively reviewed in individual chapters by experts currently active in the field. In addition, a personal history on the discovery of cytokinin is presented by Professor Folke Skoog. This volume summarizes previous findings and identifies future research directions.

In Vitro Culture of Higher Plants

In Vitro Culture of Higher Plants
Author: R.L.M Pierik
Publisher: Springer Science & Business Media
Total Pages: 346
Release: 2012-12-06
Genre: Science
ISBN: 9401157502

In Vitro Culture of Higher Plants presents an up-to-date and wide- ranging account of the techniques and applications, and has primarily been written in response to practical problems. Special attention has been paid to the educational aspects. Typical methodological aspects are given in the first part: laboratory set-up, composition and preparation of media, sterilization of media and plant material, isolation and (sub)culture, mechanization, the influence of plant and environmental factors on growth and development, the transfer from test-tube to soil, aids to study. The question of why in vitro culture is practised is covered in the second part: embryo culture, germination of orchid seeds, mericloning of orchids, production of disease-free plants, vegetative propagation, somaclonal variation, test-tube fertilization, haploids, genetic manipulation, other applications in phytopathology and plant breeding, secondary metabolites.