This new edition has been updated to include the following: The use of biomarkers (organic compounds in the geospherical record with carbon skeletons) reflecting the upsurge in geoporphyrin research primarily due to MS, yeast RNA nucleic acid studies: reversed-phase HPLC of amino acids; brewing industry applications (HPLC evaluation of carotenoids in orange juice and of "debittered" citrus); HPTLC of carbohydrates; synthesis of a sweetening agent from citrus peels, synthesis and degradation of alkaloids and of sterols, GC/MS uses with sterols, petroleum products, and aromatic constituents of wine and grape juice, flash chromatography of essential oils, optical purity of enantiomers affecting flavors, fragrances, and pheromones, as well as studies of lattice inclusion compounds 1H- and 13C-NMR, MS, IR and UV data are presented for most natural products. Biomarkers—organic compounds in the geospherical record with carbon skeletons—reflecting the upsurge in geoporphyrin research primarily due to MS Yeast RNA nucleic acid studies Reversed-phase HPLC of amino acids, citrus juice components, and HPLC in brewing industry application HPTLC of carbohydrates 1H- and 13C-NMR: Sweetness evaluation and synthesis of a sweetening agent from citrus peels; seed oil sesamolin; alkaloids (strychnine, piperine, caffeine); and sterol analyses GC/MS: sterols, petroleum studies, aromatic constituents of wine and grapejuice Flash chromatography of essential oils Optical purity of enantiomers affecting flavors, fragrances, and pheromones Materials science studies of lattice inclusion compounds