Apis mellifera L., the European honey bee, is a social insect that is vital to managed pollination services in the United States. Honey bee health and colony survival are challenged by the novel parasite Varroa destructor (Anderson & Trueman 2000). Honey bee queens are naturally polyandrous, mating with an average of 12 males. Hyper-polyandrous queens, created via artificial insemination, have shown increased resistance to Varroa mites. This project combined a hyper-polyandry treatment with a genetic treatment of bees selected for Varroa Sensitive Hygienic (VSH) trait, an additive rare resistance trait. The combination of these two intrinsic forms of resistance resulted in colonies with lower Varroa mite levels and these findings offer support to the rare-allele hypothesis for the evolution of extreme polyandry in honey bees. Additionally, an applied field method was evaluated to simulate the benefits of hyper-polyandry via brood mixing: the manual sharing of immature bees between a set of colonies to increase genetic diversity without artificial insemination. Brood mixing did not have a significant effect on any measure of colony strength and the lack of similar results to relevant tests of hyper-polyandry in the United Kingdom may be due to the homogeneous genetic structure of the United States honey bee population and indicates a lack of heterogeneous genetics when sourcing queen stock from individual honey bee queen breeders.