The Protein Protocols Handbook
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Author | : John M. Walker |
Publisher | : Springer Science & Business Media |
Total Pages | : 759 |
Release | : 2008-02-12 |
Genre | : Science |
ISBN | : 1603272593 |
In The Protein Protocols Handbook, I have attempted to provide a cross-section of analytical techniques commonly used for proteins and peptides, thus providing a benehtop manual and guide both for those who are new to the protein chemistry laboratory and for those more established workers who wish to use a technique for the first time. We each, of course, have our own favorite, commonly used gel system, g- staining method, blotting method, and so on; I'm sure you will find yours here. H- ever, I have also described a variety of altematives for many of these techniques; though they may not be superior to the methods you commonly use, they may nev- theless be more appropriate in a particular situation. Only by knowing the range of techniques that are available to you, and the strengths and limitations of these te- niques, will you be able to choose the method that best suits your purpose.
Author | : John M. Walker |
Publisher | : Springer |
Total Pages | : 1099 |
Release | : 2007-10-02 |
Genre | : Science |
ISBN | : 1592591698 |
The Protein Protocols Handbook, Second Edition aims to provide a cross-section of analytical techniques commonly used for proteins and peptides, thus providing a benchtop manual and guide for those who are new to the protein chemistry laboratory and for those more established workers who wish to use a technique for the first time. All chapters are written in the same format as that used in the Methods in Molecular BiologyTM series. Each chapter opens with a description of the basic theory behind the method being described. The Materials section lists all the chemicals, reagents, buffers, and other materials necessary for carrying out the protocol. Since the principal goal of the book is to provide experimentalists with a full account of the practical steps necessary for carrying out each protocol successfully, the Methods section contains detailed st- by-step descriptions of every protocol that should result in the successful execution of each method. The Notes section complements the Methods material by indicating how best to deal with any problem or difficulty that may arise when using a given technique, and how to go about making the widest variety of modifications or alterations to the protocol. Since the first edition of this book was published in 1996 there have, of course, been significant developments in the field of protein chemistry.
Author | : John M. Walker |
Publisher | : Humana Press |
Total Pages | : 490 |
Release | : 1994-04-26 |
Genre | : Science |
ISBN | : 9780896032699 |
Basic Protein and Peptide Protocols offers an excellent collection of reproducible, step-by-step laboratory methods covering three major areas: (1) the quantitation and characterization of proteins, (2) the electrophoretic and blotting procedures used in protein isolation and characterization, and (3) the analysis of protein and peptide structure. THOUSANDS of labs are already using Basic Protein and Peptide Protocols-you should be too!
Author | : Walker |
Publisher | : |
Total Pages | : 1176 |
Release | : 2007-09-01 |
Genre | : |
ISBN | : 9788181287670 |
Author | : Robert Allen Copeland |
Publisher | : Kluwer Academic Pub |
Total Pages | : 228 |
Release | : 1994 |
Genre | : Science |
ISBN | : 9780412037412 |
A laboratory manual detailing the methods most useful to chemists, biochemists and molecular biologists analyzing the purity and structural integrity of proteins. Assumes no specialized equipment or expertise in proteins. Some background in also provided, but the basic science is not covered. Spiral bound to lie flat. Annotation copyright by Book News, Inc., Portland, OR
Author | : John E. Coligan |
Publisher | : |
Total Pages | : |
Release | : 1995 |
Genre | : |
ISBN | : |
Author | : John M. Walker |
Publisher | : Springer Science & Business Media |
Total Pages | : 969 |
Release | : 2005-03-09 |
Genre | : Medical |
ISBN | : 1588293432 |
Hands-on researchers describe in step-by-step detail a wide range of proven laboratory methods and bioinformatics tools essential for analysis of the proteome. These cutting-edge techniques (73 in all) addresses such important tasks as sample preparation, 2D-PAGE, gel staining, protein expression profiling, identifying protein-protein interactions, and protein chip tehcnology, as well as a range of newly developed methodologies for determing the structure and function of a protein, including novel mass spectrometry and LC-MS techniques, protein array technology, and a variety of structural and functional proteomics techniques needed to determine the function of newly discovered protein sequences.
Author | : Shawn Doonan |
Publisher | : Springer Science & Business Media |
Total Pages | : 406 |
Release | : 2008-02-02 |
Genre | : Science |
ISBN | : 1592595456 |
Hans Neurath has written that this is the second golden era of enzymology {Protein Science [1994], vol. 3, pp. 1734—1739); he could with justice have been more general and referred to the second golden age of protein chemistry. The last two decades have seen enormous advances in our understanding of the structures and functions of pro teins arising on the one hand from improvements and developments in analytical techniques {see the companion volume, Basic Protein and Peptide Protocols, in this series) and on the other hand from the tech nologies of molecular genetics. Far from turning the focus away from protein science, the ability to isolate, analyze, and express genes has increased interest in proteins as gene products. Hence, many laborato ries are now getting involved in protein isolation for the first time, either as an essential adjunct to their work in molecular genetics or because of a curiosity to know more about the products of the genes that they have been studying. Protein Purification Protocols is aimed mainly at these newcom ers to protein purification, but it is hoped that it will also be of value to established practitioners who may find here techniques that they have not tried, but which might well be most applicable in their work. With the exception mainly of the first and last chapters, the format of the contributions to the present book conform to the established format of the Methods in Molecular Biology series.
Author | : Roger A. Clegg |
Publisher | : Springer Science & Business Media |
Total Pages | : 352 |
Release | : 1998 |
Genre | : Medical |
ISBN | : 9780896034877 |
In Protein Targeting Protocols, master experimentalists describe, largely from the perspective of intracellular signaling, their best time-tested methods for detecting and analyzing the proteins and protein motifs involved in cellular targeting interactions. The techniques range from basic methods that reveal the existence of targeting interactions and characterize physicochemical properties to those for analyzing the more structurally elaborate systems involved in the movement of molecules between cellular compartments, with examples taken from nuclear transport, clathrin-coated vesicle traffic, and secretory sorting. The themes of targeting domains, particularly the SH-2, SH-3, and PH domains, and the principal targeting of lipid modifications-myristoylation, reversible palmitoylation, prenylation-are fully explored. Protein Targeting Protocols offers a valuable collection of both basic and state-of-the-art techniques suitable for use in all areas of molecular cell biology by researchers interested in defining and characterizing the wide variety of protein interactions underlying targeting mechanisms. Readers will find it serves well as a firm methodological foundation for laboratories newly entering this field and as an indispensable reference work for those already well-established in it.
Author | : John E. Coligan |
Publisher | : Current Protocols |
Total Pages | : |
Release | : 1995 |
Genre | : Science |
ISBN | : 9780471111849 |
Scientists across disciplines have increasingly come to recognize the power of the protein. Current Protocols in Protein Science, a two-volume looseleaf manual, was developed in response to this revitalized interest and provides the most comprehensive collection of expert protein methods available. The publication covers both basic and advanced methods used in protein purification, characterization, and analysis as well as post-translational modification and structural analysis. More than 800 basic, support and alternate protocols have been carefully chosen for maximum applicability. Carefully edited, step-by-step protocols replete with material lists, expert commentaries, and safety and troubleshooting tips ensure that you can duplicate the experimental results in your own laboratory. Quarterly updates, which are filed into the looseleaf, keep the set current with the latest developments in protein science methods. The initial purchase includes one year of updates and then subscribers may renew their annual subscriptions. Current Protocols publishes a family of laboratory manuals for bioscientists, including Molecular Biology, Immunology, Human Genetics, Cytometry, Cell Biology, Neuroscience, Pharmacology, and Toxicology.