The Paradigm Of Understanding Engineering And Delivering Dna Binding Proteins
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| Author | : Joshua Farrell Meckler |
| Publisher | : |
| Total Pages | : |
| Release | : 2015 |
| Genre | : |
| ISBN | : 9781339542683 |
Engineered DNA-binding proteins have put unprecedented power into the hands of molecular biologists. In a blistering five-year span, the field has seen a revolution in technologies, from the hard-to-construct Zinc Fingers, through the extremely effective TAL Effector (TALE) proteins, to the incredibly simple Cas9 platform. These three proteins, co-opted from nature, are entirely distinct in their origins: ZFs are eukaryotic transcription factors, TALEs are invasive bacterial proteins, and Cas9 is part of a prokaryotic immune system. Still, as different as their sources and even their modes of binding, they share a modularity and programmability that has made them ideal for genome engineering. In understanding and then utilizing these tools for biotechnological purposes, investigators have followed a paradigm: understand the binding, build targeted tools upon the framework, and finally, deploy the tools for therapy. If one looks at papers in the genome engineering field, it's not uncommon to see the same study mirrored for these three platforms. Here, we trailblaze our own course along this well-worn pathway. We first biochemically investigate the binding of TAL Effectors, quantifying the relative affinities of their modular repeats and revealing an unexpected polarity of binding. Next, we take a catalytically inactive Cas9 nuclease and we convert it to a targeted gene-regulation tool that is active at endogenous loci in human cells. And finally, we take up the challenge of delivery and demonstrate successful protein-mediated transduction of both TALEs and Cas9.
| Author | : Morgan Lee Maeder |
| Publisher | : |
| Total Pages | : |
| Release | : 2013 |
| Genre | : |
| ISBN | : |
Engineered DNA-binding proteins enable targeted manipulation of the genome. Zinc fingers are the most well characterized DNA-binding domain and for many years research has focused on understanding and manipulating the sequence-specificities of these proteins. Recently, major advances in the ability to engineer zinc finger proteins, as well as the discovery of a new class of DNA-binding domains - transcription activator-like effectors (TALEs), have made it possible to rapidly and reliably engineer proteins targeted to any sequence of interest. With this capability, focus has shifted to exploring the applications of this powerful technology. In this dissertation I explore three important applications of engineered DNA-binding proteins.
| Author | : Kevin Healy |
| Publisher | : Elsevier |
| Total Pages | : 4865 |
| Release | : 2017-05-18 |
| Genre | : Technology & Engineering |
| ISBN | : 0081006926 |
Comprehensive Biomaterials II, Second Edition, Seven Volume Set brings together the myriad facets of biomaterials into one expertly-written series of edited volumes. Articles address the current status of nearly all biomaterials in the field, their strengths and weaknesses, their future prospects, appropriate analytical methods and testing, device applications and performance, emerging candidate materials as competitors and disruptive technologies, research and development, regulatory management, commercial aspects, and applications, including medical applications. Detailed coverage is given to both new and emerging areas and the latest research in more traditional areas of the field. Particular attention is given to those areas in which major recent developments have taken place. This new edition, with 75% new or updated articles, will provide biomedical scientists in industry, government, academia, and research organizations with an accurate perspective on the field in a manner that is both accessible and thorough. Reviews the current status of nearly all biomaterials in the field by analyzing their strengths and weaknesses, performance, and future prospects Covers all significant emerging technologies in areas such as 3D printing of tissues, organs and scaffolds, cell encapsulation; multimodal delivery, cancer/vaccine - biomaterial applications, neural interface understanding, materials used for in situ imaging, and infection prevention and treatment Effectively describes the many modern aspects of biomaterials from basic science, to clinical applications
| Author | : TALWAR, G.P. |
| Publisher | : PHI Learning Pvt. Ltd. |
| Total Pages | : 1593 |
| Release | : 2015 |
| Genre | : Medical |
| ISBN | : 8120351258 |
The Fourth Edition of the compendium pools together the knowledge and experience of experts from all over the world, who are engaged in teaching and research in the field of biochemistry, medical sciences and allied disciplines. Comprising 20 sections, the present edition of the book has been substantially revised incorporating the latest research and achievements in the field. Beginning appropriately with chemical architecture of the living systems, role and significance of biochemical reactions, organization of specialised tissues, and importance of food and nutrition, the book explores beyond traditional boundaries of biochemistry. The knowledge of various organ systems has been expanded covering their normal function, ailments and dysfunction. A chapter on Eye and Vision explaining molecular basis of cataract and glaucoma have been added. Also, the book introduces stem cells and regenerative therapy and defines molecules associated with pleasure, happiness, stress and anxiety. A Section on Gastrointestinal and Biliary System elaborates on physiology and dysfunction including fatty liver and its implications, and hepatitis viruses. The knowledge of Human Genetics and Biochemical Basis of Inheritance has been appropriately expanded to reflect the latest advances in various domains. Besides DNA fingerprinting for identity establishment, the Section discusses epigenetics, micro-RNA and siRNA including their role in gene expression, chromatin modification and its association with human diseases, and genetic engineering. It also explores emerging areas such as metabolomics and proteomics; synthetic biology; and dual use technology in bioterrorism. Due emphasis has been given to the Section on Cell Replication and Cancer. Emergence of the use of probiotics in human health has also been highlighted. Besides, an entire Section has been devoted to male and female reproductive systems, fertilization, implantation, pregnancy, lactation, and assisted reproductive technology. Immunology, including vaccines and immunization, has been given due attention with latest updates in this fast growing area. Modern medicine, despite its stupendous advances cannot provide cure for all ailments. Thus, the new edition provides knowledge of alternative medicine systems—Ayurveda, Homeopathy, Unani, Yoga and Herbal Medicine. Incorporating vast information on the latest and emerging areas, the book will be of immense value to the students of medical sciences not only in their preclinical years, but also in all phases of medical course including postgraduate education and practice. Besides, it will also serve as a valuable source to the students of biochemistry and human bi
| Author | : Aleš Prokop |
| Publisher | : Springer Science & Business Media |
| Total Pages | : 871 |
| Release | : 2011-05-26 |
| Genre | : Science |
| ISBN | : 9400712480 |
This book features a special subsection of Nanomedicine, an application of nanotechnology to achieve breakthroughs in healthcare. It exploits the improved and often novel physical, chemical and biological properties of materials only existent at the nanometer scale. As a consequence of small scale, nanosystems in most cases are efficiently uptaken by cells and appear to act at the intracellular level. Nanotechnology has the potential to improve diagnosis, treatment and follow-up of diseases, and includes targeted drug delivery and regenerative medicine; it creates new tools and methods that impact significantly upon existing conservative practices. This volume is a collection of authoritative reviews. In the introductory section we define the field (intracellular delivery). Then, the fundamental routes of nanodelivery devices, cellular uptake, types of delivery devices, particularly in terms of localized cellular delivery, both for small drug molecules, macromolecular drugs and genes; at the academic and applied levels, are covered. The following section is dedicated to enhancing delivery via special targeting motifs followed by the introduction of different types of intracellular nanodelivery devices (e.g. a brief description of their chemistry) and ways of producing these different devices. Finally, we put special emphasis on particular disease states and on other biomedical applications, whilst diagnostic and sensing issues are also included. Intracellular delivery / therapy is a highly topical which will stir great interest. Intracellular delivery enables much more efficient drug delivery since the impact (on different organelles and sites) is intracellular as the drug is not supplied externally within the blood stream. There is great potential for targeted delivery with improved localized delivery and efficacy.
| Author | : Sougata Ghosh |
| Publisher | : Elsevier |
| Total Pages | : 362 |
| Release | : 2021-05-20 |
| Genre | : Technology & Engineering |
| ISBN | : 0128231157 |
Nanobiotechnology: Microbes and Plant Assisted Synthesis of Nanoparticles, Mechanisms and Applications covers in detail the green synthesis of nanostructures of tailor-made size, shape and physico-chemical and opto-electronic properties. The rationale behind the selection of bacteria, cyanobacteria, algae, fungi, virus and medicinal plants for the synthesis of biologically active exotic nanoparticles for biomedical applications is also part of this book. It also explores metal recovery, bioconversion, detoxification and removal of heavy metals using nanobiotechnology and discusses the potential of nanobiotechnology to address environmental pollution and toxicity. The book further covers the economic and commercial aspects of such green nanobiotechnology initiatives, its current status in intellectual property rights like patents filed so far globally, technology transfers, and market potential. This information enables one to decipher the scope of biogenic nanoparticles and its prospects. - Provides an overview on the general and applied aspects on nanotechnology - Gives the scope of exploring bacteria, fungi, algae, virus and medicinal plants for the synthesis of exotic nanoparticles - Furnishes a comprehensive report on the underlying molecular mechanisms behind the biosynthesis of nanoparticles - Outlines sustainable alternative strategies of bioremediation of heavy metals, metal recovery, detoxification and bioconversion using nanobiotechnology - Explores the promises of patenting, technology transfer and commercialization potential of biogenic nanoparticles
| Author | : Rocky Termanini |
| Publisher | : Academic Press |
| Total Pages | : 484 |
| Release | : 2020-08-18 |
| Genre | : Science |
| ISBN | : 012823458X |
Storing Digital Binary Data into Cellular DNA demonstrates how current digital information storage systems have short longevity and limited capacity, also pointing out that their production and consumption of data exceeds supply. Author Rocky Termanini explains the DNA system and how it encodes vast amounts of data, then presents information on the emergence of DNA as a storage technology for the ever-growing stream of data being produced and consumed. The book will be of interest to a range of readers looking to understand this game-changing technology, including researchers in computer science, biomedical engineers, geneticists, physicians, clinicians, law enforcement and cybersecurity experts. - Presents a comprehensive reference on the fascinating and emerging technology of DNA storage - Helps readers understand key concepts on how DNA works as an information storage system - Provides readers with key information on the technologies used to work with DNA data encoding, such as CRISPR - Covers emerging areas of application and ethical concern, such as Smart Cities, cybercrime and cyberwarfare - Includes coverage of synthesizing DNA-encoded data, sequencing DNA-encoded data, and fusing DNA with Digital Immunity Ecosystems (DIE)
| Author | : |
| Publisher | : |
| Total Pages | : 1940 |
| Release | : 2004 |
| Genre | : Medicine |
| ISBN | : |
Vols. for 1963- include as pt. 2 of the Jan. issue: Medical subject headings.
| Author | : |
| Publisher | : |
| Total Pages | : 840 |
| Release | : 2006 |
| Genre | : Genetic engineering |
| ISBN | : |
| Author | : Daan J. A. Crommelin |
| Publisher | : Springer |
| Total Pages | : 661 |
| Release | : 2019-04-13 |
| Genre | : Medical |
| ISBN | : 3030007103 |
This introductory text explains both the basic science and the applications of biotechnology-derived pharmaceuticals, with special emphasis on their clinical use. It serves as a complete one-stop source for undergraduate/graduate pharmacists, pharmaceutical science students, and for those in the pharmaceutical industry. The Fifth Edition completely updates the previous edition, and also includes additional coverage on the newer approaches such as oligonucleotides, siRNA, gene therapy and nanotech and enzyme replacement therapy.
