Enzyme Histochemistry

Enzyme Histochemistry
Author: Z. Lojda
Publisher: Springer Science & Business Media
Total Pages: 350
Release: 2012-12-06
Genre: Medical
ISBN: 3642672345

During recent years enzyme histochemical reactions have increasingly been considered as important, the reason being that enzyme histo chemistry is now a well-established link between morphology and bio chemistry. The development of numerous new methods and in particular the improvement of existing techniques contributed to the expansion of enzyme histochemical reactions. Today, the use of these methods allows detailed insight into molecular processes of single cells and their constituents. The selection of a suitable method for enzyme histochemical investigations needs thorough knowledge and critical evaluation of the reactions de scribed for the histochemical demonstration of enzymes and introduced in laboratory practice. Often, it is difficult for scientists primarily concerned with the application of methods and for laboratory assistants to comment on the value of an enzyme histochemical reaction. Our book will serve as a guide in this respect. It contains the most important histochemical methods for the localization of enzymes, all of which were checked by the authors themselves. These methods were often modified and frequently used for numerous different investigations of healthy and diseased organs in basic research and in routine practice.

Histological & Histochemical Methods

Histological & Histochemical Methods
Author: John Alan Kiernan
Publisher: Pergamon
Total Pages: 454
Release: 1990
Genre: Medical
ISBN:

Revised and updated edition (1st was 1981) of a textbook on chemical and physical principles of fixation, staining and histochemistry. For students i all biological subjects using histological techniques, as well as researcher and medical laboratory technologists. Annotation copyright Book News, Inc

Pancreatic Islet Biology

Pancreatic Islet Biology
Author: Anandwardhan A. Hardikar
Publisher: Springer
Total Pages: 332
Release: 2016-10-25
Genre: Science
ISBN: 3319453076

This comprehensive volume discusses in vitro laboratory development of insulin-producing cells. It encompasses multiple aspects of islet biology—from embryonic development and stem cell differentiation to clinical studies in islet transplantation, regulation of islet beta-cell regeneration, pancreatic progenitors, mathematical modelling of islet development, epigenetic regulation, and much more. The chapter authors represent leading laboratories from around the world who contribute their international perspectives and global expertise. Collectively, they provide the reader with a concise yet detailed knowledge of processes and current developments in islet regenerative biology. Pancreatic Islet Biology, part of the Stem Cell Biology and Regenerative Medicine series, is essential reading for researchers and clinicians in stem cells or endocrinology, especially those focusing on diabetes.

Histochemistry of Single Molecules

Histochemistry of Single Molecules
Author: Carlo Pellicciari
Publisher: Springer Nature
Total Pages: 351
Release: 2022-09-24
Genre: Science
ISBN: 1071626752

This volume details histochemical techniques for the detection of specific molecules or metabolic processes, both at light and electron microscopy. Chapters are divided into seven sections covering Vital histochemistry, Carbohydrate histochemistry, Protein histochemistry, Lipid histochemistry, Nuclear histochemistry, Plant histochemistry and Histochemistry for Nanoscience. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. The volume also contains three discursive chapters on Histochemistry in advanced cytometry, Lectins and Detection of molecules in plant cell walls by fluorescence microscopy. Authoritative and cutting-edge, Histochemistry of Single Molecules: Methods and Protocols, Second Edition aims to be a useful practical guide for researchers to help further their study in this field.

Immunohistochemistry and Immunocytochemistry

Immunohistochemistry and Immunocytochemistry
Author: Simon Renshaw
Publisher: John Wiley & Sons
Total Pages: 258
Release: 2017-02-06
Genre: Science
ISBN: 1118717775

Immunohistochemistry and immunocytochemistry are invaluable tools for the visualization of tissue and cellular antigens in diagnostic and biological research environments. The need to obtain accurate, reliable and reproducible results is paramount. It is with this fundamental aim in mind that we have compiled Immunohistochemistry: Essential Methods. We have achieved this by examining each aspect of immunochemistry in turn, with each chapter including detailed information regarding the subject matter in question. Each chapter is written by an expert in their field and includes protocols that are typically used in their own research. Subjects covered are, amongst others, antibodies and their production; selection of reporter labels; immunochemical staining methods and experimental design (both using single and multiple reporter labels); quality assurance; automated immunochemistry; confocal microscopy and electron microscopy. In addition, benefits and limitations of each approach are discussed within the chapters.

Enzyme Histochemistry

Enzyme Histochemistry
Author: Cornelis J. F. van Noorden
Publisher: Oxford University Press, USA
Total Pages: 136
Release: 1992
Genre: Business & Economics
ISBN:

The functional analysis of macromolecular structures in tissues and cells has been greatly enhanced by advances in histochemistry and cytochemistry. Enzyme histochemistry is becoming particularly important as new methods succeed in demonstrating and quantifying the activity of key regulatory enzymes. The specificity, precision, and reproducibility of enzyme histochemical methods are constantly improving. This practical laboratory handbook contains a selection of the most important enzyme histochemical techniques currently available for light microscopy. The methods included here were chosen because of their reliability and specificity, and all are clearly detailed in easy-to-follow protocols. The introductory chapter provides a good theoretical background to enzyme histochemistry, and the book will be of interest to all researchers in cell biology, pathology, biochemistry, and cell physiology.

Enzyme Histochemistry

Enzyme Histochemistry
Author: John D. Bancroft
Publisher: Oxford University Press, USA
Total Pages: 84
Release: 1987
Genre: Language Arts & Disciplines
ISBN:

Enzyme histochemical methods depend on visual identification of reaction products for the identification and localization of specific enzymes within tissue. This concise handbook is designed to give students, pathology, MLSO's and research workers an outline and handy lab reference to the methods which have become routine in research and hospital histopathological laboratories. The author discusses the most reliable and diagnostically important methods, and offers suggestions for further reading.

The Sugar Code

The Sugar Code
Author: Hans-Joachim Gabius
Publisher: John Wiley & Sons
Total Pages: 560
Release: 2011-09-19
Genre: Science
ISBN: 3527644946

A reader friendly overview of the structure and functional relevance of natural glycosylation and its cognate proteins (lectins), this book is also one of the few books to cover their role in health and disease. Edited by one of the pioneering experts in the field and written by a team of renowned researchers this resource is a perfect introduction for all students in life and medical sciences, biochemistry, chemistry and pharmacy. Website: WWW.WILEY-VCH.DE/HOME/THESUGARCODE

Bancroft's Theory and Practice of Histological Techniques E-Book

Bancroft's Theory and Practice of Histological Techniques E-Book
Author: Kim S Suvarna
Publisher: Elsevier Health Sciences
Total Pages: 655
Release: 2012-10-01
Genre: Medical
ISBN: 0702050326

This is a brand new edition of the leading reference work on histological techniques. It is an essential and invaluable resource suited to all those involved with histological preparations and applications, from the student to the highly experienced laboratory professional. This is a one stop reference book that the trainee histotechnologist can purchase at the beginning of his career and which will remain valuable to him as he increasingly gains experience in daily practice. Thoroughly revised and up-dated edition of the standard reference work in histotechnology that successfully integrates both theory and practice.Provides a single comprehensive resource on the tried and tested investigative techniques as well as coverage of the latest technical developments. Over 30 international expert contributors all of whom are involved in teaching, research and practice.Provides authoritative guidance on principles and practice of fixation and staining. Extensive use of summary tables, charts and boxes.Information is well set out and easy to retrieve. Six useful appendices included (SI units, solution preparation, specimen mounting, solubility). Provides practical information on measurements, preparation solutions that are used in daily laboratory practice. Color photomicrographs used extensively throughout. Better replicates the actual appearance of the specimen under the microscope. Brand new co-editors. New material on immunohistochemical and molecular diagnostic techniques.Enables user to keep abreast of latest advances in the field.