The market value of cotton is largely determined by a subjective evaluation of a limited number of quality parameters such as: Grade, Length, and Fineness. Cotton is classed to determine its grade, which indicates the spinning value and utility, and hence the market value or price. Farmers are interested in the classification of cotton by grade to evaluate production, harvesting, and ginning practices, in order to market their cotton profitably, on the other hand, merchants could efficiently buy and sell cotton in accordance with grade.The world wide spread of High Volume Instrument (HVI) testing systems, with their ability to provide rapid and economical measurements of the most important cotton fiber properties, has stimulated efforts to obtain the maximum benefits from these systems.There is a direct correlation between the quality of raw material and the end products. The lower quality of cotton fibers means the lower quality of yarn produced from such a raw material. High quality cotton blends are superior with respect to properties such as length, fineness, elongation, and brightness, sufficiently mature and without any trash particles and displaying a high capacity of spinning consistency.It's worth to say that we are in a need for the visual classification, as it is suitable for application in the local marketing system, as producer sells seed cotton. While using the fiber quality index (FQI) comes in a following step, as it is suitable for application in the international marketing system, as traders sells lint cotton.The present investigations are subjected to study the relationship between the HVI fiber properties and yarn quality to evaluate the actual value of the cotton fibers. Besides determining the relative importance or contribution percentage of each fiber property to the yarn quality and suggesting a statistical approach for calculating a Fiber Quality Index (FQI). In Addition to study the effect of variety, location and seed cotton level on the fiber quality index of the Egyptian cotton.Also to clarify the effect of both genotype and environment on the HVI cotton fiber properties of some Egyptian cotton varieties and its yarn quality. Besides, introducing some new variables fiber quality index (FQI) for determining the actual value of cotton using the HVI fiber properties. This FQI defined the desirable properties of cotton that allow the production of the best quality yarn in particular manufacturing system.New FQIs (Fiber Quality Indices) equations, Lea Product value predicting equation, and a New Leaf Grade equation was obtained, beside studying Card Waste (%) effect on color attributes.