DNA-mediated Gold Nanoparticle Assembly

DNA-mediated Gold Nanoparticle Assembly
Author: Kyrylo Zagorovsky
Publisher:
Total Pages:
Release: 2016
Genre:
ISBN:

Unique physicochemical properties of nanomaterials offer novel methodologies for disease treatment and diagnosis. Dynamic nanoparticle assemblies are adding an additional level of functionality and flexibility by combining multiple nanoparticle types into structures with stimuli-responsive size and surface chemistry. DNA-mediated gold nanoparticles assemblies are offering the most functional and versatile platform, which combines DNA addressability with large surface area, monodispersity, and easy functionalization of gold nanoparticles. Assembly size determines their application, with smaller nanoassemblies particularly suitable for drug delivery, and larger microassemblies used for biosensing. Despite a number of published studies, no in vivo examples or clinically ready biosensors have been reported. Therefore, the goal of this thesis was to identify the barriers for translation in both fields and to develop methods to overcome these limitations. To this end core-satellite nanoassemblies were designed for in vivo delivery, which efficiently protected loaded cargo and minimized macrophage recognition. Their controllable serum stability could be used as the mechanism of cargo release. In vivo nanoassemblies showed longer circulation half-life, improved tumour targeting, and renal bioelimination. In parallel, larger microassembly structures were combined with DNA enzyme signal amplification to generate a novel infectious pathogen biosensor. The assay was particularly suited for point-of-care setting with cheap and stable components and simple, fast, and specific colourimetric readout that could assay multiple targets in parallel. To demonstrate its clinical readiness, the assay was adapted for detection of a panel of five urinary tract infection bacteria. All targets were successfully identified in parallel at clinically relevant concentrations. Preliminary tests with E. coli patient samples confirmed clinical detection with good sensitivity and excellent specificity. This work presents a novel DNA-mediated gold nanoparticle assembly platform that is ready for in vivo translation and clinical in vitro diagnostics.

Gold Nanostars

Gold Nanostars
Author: Giuseppe Chirico
Publisher: Springer
Total Pages: 93
Release: 2015-08-03
Genre: Technology & Engineering
ISBN: 3319207687

This Brief focuses on the synthesis, functionalization techniques, optical properties and biomedical application of gold nanostars (GNS). Various facilities of gold nanostars synthesis as well as functionalization of GNS with PEG, organic dyes, bioactive compounds are discussed. The authors discuss physical origin of the Localized Surface Plasmon Resonances and the way the nano-environment affects them. The implication of the LSPR of gold nanostars surface enhanced Raman scattering is also discussed. The emphasis has been done on the application of GNS for current and emerge needs of medicine, biology and pharmacy. Moreover, properties of gold nanostars as contrast agents for in vivo imaging and interaction of GNS with cells are also discussed in this Brief.

Assemblies of Gold Nanoparticles at Liquid-Liquid Interfaces

Assemblies of Gold Nanoparticles at Liquid-Liquid Interfaces
Author: Evgeny Smirnov
Publisher: Springer
Total Pages: 270
Release: 2018-04-19
Genre: Technology & Engineering
ISBN: 3319779141

This book is devoted to various aspects of self-assembly of gold nanoparticles at liquid-liquid interfaces and investigation of their properties. It covers primarily two large fields: (i) self-assembly of nanoparticles and optical properties of these assemblies; and (ii) the role of nanoparticles in redox electrocatalysis at liquid-liquid interfaces. The first part aroused from a long-lasting idea to manipulate adsorption of nanoparticles at liquid-liquid with an external electric field to form 'smart' mirrors and/or filters. Therefore, Chapters 3 to 5 are dedicated to explore fundamental aspects of charged nanoparticles self-assembly and to investigate optical properties (extinction and reflectance) in a through manner. Novel tetrathiafulvalene (TTF)-assisted method leads to self-assembly of nanoparticles into cm-scale nanofilms or, so-called, metal liquid-like droplets (MeLLDs) with remarkable optical properties. The second part (Chapters 6 to 8) clarifies the role of nanoparticles in interfacial electron transfer reactions. They demonstrate how nanoparticles are charged and discharged upon equilibration of Fermi levels with redox couples in solution and how it can be used to perform HER and ORR. Finally, Chapter 9 gives a perspective outlook, including applications of suggested methods in fast, one-step preparation of colloidosomes, SERS substrates as well as pioneer studies on so-called Marangony-type shutters drive by the electric field.

Self-Assembly

Self-Assembly
Author: Ramanathan Nagarajan
Publisher: John Wiley & Sons
Total Pages: 364
Release: 2019-01-07
Genre: Science
ISBN: 1119001366

An introduction to the state-of-the-art of the diverse self-assembly systems Self-Assembly: From Surfactants to Nanoparticles provides an effective entry for new researchers into this exciting field while also giving the state of the art assessment of the diverse self-assembling systems for those already engaged in this research. Over the last twenty years, self-assembly has emerged as a distinct science/technology field, going well beyond the classical surfactant and block copolymer molecules, and encompassing much larger and complex molecular, biomolecular and nanoparticle systems. Within its ten chapters, each contributed by pioneers of the respective research topics, the book: Discusses the fundamental physical chemical principles that govern the formation and properties of self-assembled systems Describes important experimental techniques to characterize the properties of self-assembled systems, particularly the nature of molecular organization and structure at the nano, meso or micro scales. Provides the first exhaustive accounting of self-assembly derived from various kinds of biomolecules including peptides, DNA and proteins. Outlines methods of synthesis and functionalization of self-assembled nanoparticles and the further self-assembly of the nanoparticles into one, two or three dimensional materials. Explores numerous potential applications of self-assembled structures including nanomedicine applications of drug delivery, imaging, molecular diagnostics and theranostics, and design of materials to specification such as smart responsive materials and self-healing materials. Highlights the unifying as well as contrasting features of self-assembly, as we move from surfactant molecules to nanoparticles. Written for students and academic and industrial scientists and engineers, by pioneers of the research field, Self-Assembly: From Surfactants to Nanoparticles is a comprehensive resource on diverse self-assembly systems, that is simultaneously introductory as well as the state of the art.

Comprehensive Nanoscience and Technology

Comprehensive Nanoscience and Technology
Author:
Publisher: Academic Press
Total Pages: 2785
Release: 2010-10-29
Genre: Science
ISBN: 0123743966

From the Introduction: Nanotechnology and its underpinning sciences are progressing with unprecedented rapidity. With technical advances in a variety of nanoscale fabrication and manipulation technologies, the whole topical area is maturing into a vibrant field that is generating new scientific research and a burgeoning range of commercial applications, with an annual market already at the trillion dollar threshold. The means of fabricating and controlling matter on the nanoscale afford striking and unprecedented opportunities to exploit a variety of exotic phenomena such as quantum, nanophotonic and nanoelectromechanical effects. Moreover, researchers are elucidating new perspectives on the electronic and optical properties of matter because of the way that nanoscale materials bridge the disparate theories describing molecules and bulk matter. Surface phenomena also gain a greatly increased significance; even the well-known link between chemical reactivity and surface-to-volume ratio becomes a major determinant of physical properties, when it operates over nanoscale dimensions. Against this background, this comprehensive work is designed to address the need for a dynamic, authoritative and readily accessible source of information, capturing the full breadth of the subject. Its six volumes, covering a broad spectrum of disciplines including material sciences, chemistry, physics and life sciences, have been written and edited by an outstanding team of international experts. Addressing an extensive, cross-disciplinary audience, each chapter aims to cover key developments in a scholarly, readable and critical style, providing an indispensible first point of entry to the literature for scientists and technologists from interdisciplinary fields. The work focuses on the major classes of nanomaterials in terms of their synthesis, structure and applications, reviewing nanomaterials and their respective technologies in well-structured and comprehensive articles with extensive cross-references. It has been a constant surprise and delight to have found, amongst the rapidly escalating number who work in nanoscience and technology, so many highly esteemed authors willing to contribute. Sharing our anticipation of a major addition to the literature, they have also captured the excitement of the field itself in each carefully crafted chapter. Along with our painstaking and meticulous volume editors, full credit for the success of this enterprise must go to these individuals, together with our thanks for (largely) adhering to the given deadlines. Lastly, we record our sincere thanks and appreciation for the skills and professionalism of the numerous Elsevier staff who have been involved in this project, notably Fiona Geraghty, Megan Palmer and Greg Harris, and especially Donna De Weerd-Wilson who has steered it through from its inception. We have greatly enjoyed working with them all, as we have with each other.

Handbook of Nanofabrication

Handbook of Nanofabrication
Author:
Publisher: Academic Press
Total Pages: 307
Release: 2010-05-25
Genre: Technology & Engineering
ISBN: 0123751772

Many of the devices and systems used in modern industry are becoming progressively smaller and have reached the nanoscale domain. Nanofabrication aims at building nanoscale structures, which can act as components, devices, or systems, in large quantities at potentially low cost. Nanofabrication is vital to all nanotechnology fields, especially for the realization of nanotechnology that involves the traditional areas across engineering and science. - Includes chapters covering the most important Nanofabrication techniques, which aids comprehensive understanding of the latest manufacturing technologies encountered in the field of nano-level manufacturing which is essential for preparing for advanced study and application in nanofabrication techniques by enabling thorough understanding of the entire nanofabrication process as it applies to advanced electronic and related manufacturing technologies - Each chapter covers a nanofabrication technique comprehensively, which allows the reader to learn to produce nanometer-level products as well as collect, process, and analyze data, improve process parameters, and how to assist engineers in research, development and manufacture of the same - Includes contributions from recognized experts from around the globe, making the reader aware of variations in similar techniques applied in different geographical locations and is better positioned to establish all possible global applications

Gold Nanoparticles

Gold Nanoparticles
Author: Abagail Jarnagin
Publisher: Nova Science Publishers
Total Pages: 0
Release: 2013
Genre: Gold
ISBN: 9781622579273

In this book, the authors present current research in the study of the synthesis, optical properties and applications for cancer treatment of gold nanoparticles. Topics discussed include the use of gold nanoparticles in cancer treatment and biomedical applications to target tumors and provide detection, drug carriers, gene silencing and radiotherapy; gold nanoparticle fabrication by laser ablation technique and their optical and morphological study; gold nanoparticles for metabolite imaging; formation of gold nanoparticles inside the corona of amphiphilic triblock copolymer micelles; and the intracellular delivery of gold nanoparticles and their application in nanomedicine.

Nanotechnology in Catalysis 3

Nanotechnology in Catalysis 3
Author: Bing Zhou
Publisher: Springer Science & Business Media
Total Pages: 342
Release: 2007-09-05
Genre: Technology & Engineering
ISBN: 0387346880

This volume continues the tradition formed in Nanotechnology in Catalysis 1 and 2. As with those books, this one is based upon an ACS symposium. Some of the most illustrious names in heterogeneous catalysis are among the contributors. The book covers: Design, synthesis, and control of catalysts at nanoscale; understanding of catalytic reaction at nanometer scale; characterization of nanomaterials as catalysts; nanoparticle metal or metal oxides catalysts; nanomaterials as catalyst supports; new catalytic applications of nanomaterials.