Overgrazing and fire suppression has left much rangeland in poor condition for various wildlife species. Prescribed fire is one range improvement practice used to restore degraded wildlife habitat. I determined the effect of prescribed fire on whitetailed deer (Odocoileus virginianus) spatial and temporal distribution, in the presence of cattle grazing. Three 40 ha patches, constituting 10% and 6% of the land area in the lesser and greater Yellow Bluff pasture, respectively, were burned in September 2005. To determine habitat use and distribution of deer relative to these burns 3 bucks and 3 does were netted from a helicopter and fitted with Global Positioning System (GPS) telemetry collars (Lotek GPS_3300S) for a period of 30 days during each season. For estimation of spatial distribution of deer, the collars were programmed to take a position fix every hour to reduce problems associated with spatial autocorrelation. For 12 days within this period the collars recorded animal location every 5 minutes to compare habitat use with 6-9 GPS collars (GPS_3300LR) placed on cattle. This allowed me to examine fine-scale movements of deer relative to cattle.