Segregation of hot mix asphalt is a recurring problem in the paving industry. There is little documented research that quantifies the effects of segregation on mix properties and performance. The Asphalt Pavement Analyzer (APA) is a new class of pavement tester available to evaluate pavement performance. The APA can evaluate the rutting potential, moisture susceptibility and fatigue life of bituminous mixtures. Materials were available from a previous study (K-TRAN: KU-96-6) on segregation for use in evaluating the use of the APA with Kansas mixtures and materials. Two surface mixes, a BM-1B and BM-2, were fabricated to different levels of segregation as determined in the K-TRAN: KU-96-6 study. The mixtures were tested in the APA for rutting, moisture damage and fatigue life.