Membrane Protein Structure Determination

Membrane Protein Structure Determination
Author: Jean-Jacques Lacapère
Publisher: Methods in Molecular Biology
Total Pages: 482
Release: 2010-08-06
Genre: Science
ISBN:

Membrane proteins, representing nearly 40% of all proteins, are key components of cells involved in many cellular processes, yet only a small number of their structures have been determined. Membrane Protein Structure Determination: Methods and Protocols presents many detailed techniques for membrane protein structure determination used today by bringing together contributions from top experts in the field. Divided into five convenient sections, the book covers various strategies to purify membrane proteins, approaches to get three dimensional crystals and solve the structure by x-ray diffraction, possibilities to gain structural information for a membrane protein using electron microscopy observations, recent advances in nuclear magnetic resonance (NMR), and molecular modelling strategies that can be used either to get membrane protein structures or to move from atomic structure to a dynamic understanding of a molecular functioning mechanism. Written in the highly successful Methods in Molecular BiologyTM series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and easy to use, Membrane Protein Structure Determination: Methods and Protocols serves as an ideal reference for scientists seeking to further our knowledge of these vital and versatile proteins as well as our overall understanding of the complicated world of cell biology.

Peptide-Lipid Interactions

Peptide-Lipid Interactions
Author: Sidney A. Simon
Publisher: Academic Press
Total Pages: 606
Release: 2002-11-13
Genre: Science
ISBN: 0080925855

This volume contains a comprehensive overview of peptide-lipid interactions by leading researchers. The first part covers theoretical concepts, experimental considerations, and thermodynamics. The second part presents new results obtained through site-directed EPR, electron microscopy, NMR, isothermal calorimetry, and fluorescence quenching. The final part covers problems of biological interest, including signal transduction, membrane transport, fusion, and adhesion. Key Features * world-renowned experts * state-of-the-art experimental methods * monolayers, bilayers, biological membranes * theoretical aspects and computer simulations * rafts * synaptic transmission * membrane fusion * signal transduction

Biomembranes

Biomembranes
Author: Robert B. Gennis
Publisher: Springer Science & Business Media
Total Pages: 549
Release: 2013-04-17
Genre: Science
ISBN: 1475720653

New textbooks at all levels of chemistry appear with great regularity. Some fields like basic biochemistry, organic reaction mechanisms, and chemical thermody namics are well represented by many excellent texts, and new or revised editions are published sufficiently often to keep up with progress in research. However, some areas of chemistry, especially many of those taught at the graduate level, suffer from a real lack of up-to-date textbooks. The most serious needs occur in fields that are rapidly changing. Textbooks in these subjects usually have to be written by scientists actually involved in the research which is advancing the field. It is not often easy to persuade such individuals to set time aside to help spread the knowledge they have accumulated. Our goal, in this series, is to pinpoint areas of chemistry where recent progress has outpaced what is covered in any available textbooks, and then seek out and persuade experts in these fields to produce relatively concise but instructive introductions to their fields. These should serve the needs of one semester or one quarter graduate courses in chemistry and biochemistry. In some cases, the availability of texts in active research areas should help stimulate the creation of new courses.

Experimental Approaches of NMR Spectroscopy

Experimental Approaches of NMR Spectroscopy
Author: The Nuclear Magnetic Resonance Society of Japan
Publisher: Springer
Total Pages: 634
Release: 2017-11-23
Genre: Science
ISBN: 9811059667

This book describes the advanced developments in methodology and applications of NMR spectroscopy to life science and materials science. Experts who are leaders in the development of new methods and applications of life and material sciences have contributed an exciting range of topics that cover recent advances in structural determination of biological and material molecules, dynamic aspects of biological and material molecules, and development of novel NMR techniques, including resolution and sensitivity enhancement. First, this book particularly emphasizes the experimental details for new researchers to use NMR spectroscopy and pick up the potentials of NMR spectroscopy. Second, the book is designed for those who are involved in either developing the technique or expanding the NMR application fields by applying them to specific samples. Third, the Nuclear Magnetic Resonance Society of Japan has organized this book not only for NMR members of Japan but also for readers worldwide who are interested in using NMR spectroscopy extensively.

Solid State NMR

Solid State NMR
Author: Jerry C. C. Chan
Publisher: Springer
Total Pages: 328
Release: 2011-10-12
Genre: Science
ISBN: 3642248039

Dipolar Recoupling, by Niels Chr. Nielsen, Lasse A. Strassø and Anders B. Nielsen.- Solid-State NMR Techniques for the Structural Determination of Amyloid Fibrils, by Jerry C. C. Chan.- Solid-State 19F-NMR of Peptides in Native Membranes, by Katja Koch, Sergii Afonin, Marco Ieronimo, Marina Berditsch and Anne S. Ulrich.- Probing Quadrupolar Nuclei by Solid-State NMR Spectroscopy: Recent Advances, by Christian Fernandez and Marek Pruski.- Solid State NMR of Porous Materials Zeolites and Related Materials, by Hubert Koller and Mark Weiß.- Solid-State NMR of Inorganic Semiconductors, by James P. Yesinowski.-

Cholesterol in Membrane Models

Cholesterol in Membrane Models
Author: Leonard X. Finegold
Publisher: CRC Press
Total Pages: 298
Release: 1992-12-15
Genre: Science
ISBN: 9780849342073

Cholesterol in Membrane Models provides the most recent summary available of basic molecular models and experiments in models of biological membranes. The book introduces the cell biology of cholesterol and covers the modeling of cholesterol in model membranes by the mean field and Monte Carlo theoretical methods. The experimental movement of cholesterol into model membranes and phase diagrams of cholesterol in these membranes by nuclear magnetic resonance and by thermal techniques using different lipid chain lengths are discussed. Light and electron microscopy that explore the spatial distribution of cholesterol in the model membrane are covered. The book also examines the use of infrared/Raman and neutron spectroscopy. Cholesterol in Membrane Models is an excellent learning resource, research tool, and reference for a wide range of experimental and theoretical scientists, including cellular biologists, biophysicists, biochemists, and molecular modelers.

In-cell NMR Spectroscopy

In-cell NMR Spectroscopy
Author: Yutaka Ito
Publisher: Royal Society of Chemistry
Total Pages: 322
Release: 2019-12-09
Genre: Science
ISBN: 1839160934

In-cell NMR spectroscopy is a relatively new field. Despite its short history, recent in-cell NMR-related publications in major journals indicate that this method is receiving significant general attention. This book provides the first informative work specifically focused on in-cell NMR. It details the historical background of in-cell NMR, host cells for in-cell NMR studies, methods for in-cell biological techniques and NMR spectroscopy, applications, and future perspectives. Researchers in biochemistry, biophysics, molecular biology, cell biology, structural biology as well as NMR analysts interested in biological applications will all find this book valuable reading.

Structural Biology in Drug Discovery

Structural Biology in Drug Discovery
Author: Jean-Paul Renaud
Publisher: John Wiley & Sons
Total Pages: 1437
Release: 2020-01-09
Genre: Medical
ISBN: 1118900502

With the most comprehensive and up-to-date overview of structure-based drug discovery covering both experimental and computational approaches, Structural Biology in Drug Discovery: Methods, Techniques, and Practices describes principles, methods, applications, and emerging paradigms of structural biology as a tool for more efficient drug development. Coverage includes successful examples, academic and industry insights, novel concepts, and advances in a rapidly evolving field. The combined chapters, by authors writing from the frontlines of structural biology and drug discovery, give readers a valuable reference and resource that: Presents the benefits, limitations, and potentiality of major techniques in the field such as X-ray crystallography, NMR, neutron crystallography, cryo-EM, mass spectrometry and other biophysical techniques, and computational structural biology Includes detailed chapters on druggability, allostery, complementary use of thermodynamic and kinetic information, and powerful approaches such as structural chemogenomics and fragment-based drug design Emphasizes the need for the in-depth biophysical characterization of protein targets as well as of therapeutic proteins, and for a thorough quality assessment of experimental structures Illustrates advances in the field of established therapeutic targets like kinases, serine proteinases, GPCRs, and epigenetic proteins, and of more challenging ones like protein-protein interactions and intrinsically disordered proteins

Phospholipids Handbook

Phospholipids Handbook
Author: Gregor Cevc
Publisher: CRC Press
Total Pages: 1130
Release: 1993-08-02
Genre: Science
ISBN: 9780824790509

Employing a multidisciplinary approach to phospholipid research, this work catalogues the current knowledge of this class of molecules and details the general, chemical, physical and structural properties of phospholipid monolayers and bilayers. Phospholipid applications are also covered.