This study investigated attitudes toward and knowledge of the Americans with Disabilities Act (ADA). One hundred and thirty-three representatives from the private (for profit) and public (not for profit) sectors completed a series of paper-and-pencil measures. The malleability of ADA attitudes was also examined (70 participants received a negative and 63 participants received a neutral presentation of the ADA). As a whole, participants expressed positive attitudes toward the ADA and disability rights. Predictors of ADA attitudes included type of establishment, malleability of ADA attitudes, and knowledge of this law. More specifically, (a) public sector representatives expressed more favorable attitudes than those from the private sector; (b) participants who received the neutral ADA presentation reported more positive views than those in the negative group; and (c) participants with more ADA knowledge expressed more favorable attitudes than those with less knowledge of this law. Overall, participants demonstrated little knowledge about the ADA. Those with higher levels of academic attainment knew more about the ADA than those with lower levels of academic attainment. Lastly, acculturation to mainstream US culture was positively related to ADA knowledge. Implications of findings and directions for future research are discussed.