Separation Of Protein Mixtures By Extraction
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| Author | : Hafiz Ahmed |
| Publisher | : CRC Press |
| Total Pages | : 408 |
| Release | : 2017-07-27 |
| Genre | : Medical |
| ISBN | : 0203507436 |
Principles and Reactions of Protein Extraction, Purification, and Characterization provides the mechanisms and experimental procedures for classic to cutting-edge techniques used in protein extraction, purification, and characterization. The author presents the principles and reactions behind each procedure and uses tables to compare the different
| Author | : Carola Hunte |
| Publisher | : Elsevier |
| Total Pages | : 339 |
| Release | : 2003-01-05 |
| Genre | : Science |
| ISBN | : 0080536174 |
This second edition of Membrane Protein Purification and Crystallization, A Practical Guide is written for bench scientists working in the fields of biochemistry, biology, and proteomic research. This guide presents isolation and crystallization techniques in a concise form, emphasizing the critical aspects unique to membrane proteins. It explains the principles of the methods and provides protocols of general use, permitting researchers and students new to this area to adapt these techniques to their particular needs. This edition is not only an update but is comprised mainly of new contributions. It is the first monograph compiling the essential approaches for membrane protein crystallization, and emphasizes recent progress in production and purification of recombinant membrane proteins. - Provides general guidelines and strategies for isolation and crystallization of membrane proteins - Gives detailed protocols that have wide application, and low specialized equipment needs - Emphasizes recent progress in production and purification of recombinant membrane proteins, especially of histidine-tagged and other affinity-epitope-tagged proteins - Summarizes recent developments of Blue-Native PAGE, a high resolution separation technique, which is independent of the use of recombinant techniques, and is especially suited for proteomic analyses of membrane protein complexes - Gives detailed protocols for membrane protein crystallization, and describes the production and use of antibody fragments for high resolution crystallization - Presents a comprehensive guide to 2D-crystallization of membrane proteins
| Author | : Jörg von Hagen |
| Publisher | : John Wiley & Sons |
| Total Pages | : 498 |
| Release | : 2011-08-24 |
| Genre | : Science |
| ISBN | : 3527644695 |
This long-awaited first guide to sample preparation for proteomics studies overcomes a major bottleneck in this fast growing technique within the molecular life sciences. By addressing the topic from three different angles -- sample, method and aim of the study -- this practical reference has something for every proteomics researcher. Following an introduction to the field, the book looks at sample preparation for specific techniques and applications and finishes with a section on the preparation of sample types. For each method described, a summary of the pros and cons is given, as well as step-by-step protocols adaptable to any specific proteome analysis task.
| Author | : John F. Scamehorn |
| Publisher | : CRC Press |
| Total Pages | : 359 |
| Release | : 2020-11-25 |
| Genre | : Science |
| ISBN | : 1000103749 |
Complete with bibliographic citations and illustrations, this volume focuses on novel techniques and reviews established methods for surfactant-based separation processes that can be widely applied in industry. Describes new extraction techniques, and introduces micellar-enhanced ultrafiltration and admicellar chromatography, discusses protein extraction using reverse micelles, surfactant-enhanced carbon regeneration, and demonstrates new methods of turning waste streams containing dilute concentrations of valuable materials into product streams and examines such traditional surfactant-based methods as froth flotation and foam fractionation.
| Author | : P. Alexander |
| Publisher | : Elsevier |
| Total Pages | : 289 |
| Release | : 2014-05-15 |
| Genre | : Science |
| ISBN | : 1483149803 |
A Laboratory Manual of Analytical Methods of Protein Chemistry (Including Polypeptides), Volume 1: The Separation and Isolation of Proteins deals with the techniques used in the separation and isolation of proteins, including fractionation and characterization by dialysis, multi-membrane electrodecantation, and zonal density gradient electrophoresis. The fractionation of proteins by adsorption and ion exchange is also described. This book is comprised of seven chapters and begins with a discussion on procedures for the separation of proteins, paying particular attention to the liberation of proteins from cellular material; removal of lipids from lipoproteins; and denaturation, fractionation, and purification of proteins. The next chapter focuses on the isolation of biologically active proteins such as cytochrome, bacterial amylases, and bacterial proteinases. The reader is methodically introduced to fractionation of proteins by adsorption and ion exchange; fractionation and characterization by dialysis; multi-membrane electrodecantation; and continuous and discontinuous partition. The final chapter explains how zonal density gradient electrophoresis works as a separation method for natural mixtures of proteins, their degradation products, and other substances carrying electric charges in solution or suspension. This volume will be of interest to chemists working with proteins.
| Author | : C. Dennison |
| Publisher | : Springer Science & Business Media |
| Total Pages | : 266 |
| Release | : 2013-03-09 |
| Genre | : Science |
| ISBN | : 9401702691 |
It is a truism of science that the more fundamental the subject, the more universally applicable it is. Neverthelens, it is important to strike a level of "fundamentalness" appropriate to the task in hand. For example, an in-depth study of the mechanics of motor cars would tell one nothing about the dynamics of traffic. Traffic exists on a different "level" - it is dependent upon the existence of motor vehicles but the physics and mathematics of traffic can be adequately addressed by considering motor vehicles as mobile "blobs", with no consideration of how they become mobile. To start a discourse on traffic with a consideration of the mechanics of motor vehicles would thus be inappropropriate. In writing this volume, I have wrestled with the question of the appropriate level at which to address the physics underlying many of the techniques used in protein isolation. I have tried to strike a level as would be used by a mechanic (with perhaps a slight leaning towards an engineer) - i.e. a practical level, offering appropriate insight but with minimal mathematics. Some people involved in biochemical research have a minimal grounding in chemistry and physics and so I have tried to keep it as simple as possible.
| Author | : |
| Publisher | : |
| Total Pages | : 0 |
| Release | : 2002 |
| Genre | : Cells |
| ISBN | : 9780815332183 |
| Author | : Syed S. H. Rizvi |
| Publisher | : Elsevier |
| Total Pages | : 696 |
| Release | : 2010-10-28 |
| Genre | : Technology & Engineering |
| ISBN | : 0857090755 |
Separation, extraction and concentration are essential processes in the preparation of key food ingredients. They play a vital role in the quality optimization of common foods and beverages and there is also increasing interest in their use for the production of high-value compounds, such as bioactive peptides from milk and whey, and the recovery of co-products from food processing wastes.Part one describes the latest advances in separation, extraction and concentration techniques, including supercritical fluid extraction, process chromatography and membrane technologies. It also reviews emerging techniques of particular interest, such as pervaporation and pressurised liquid extraction. Part two then focuses on advances in separation technologies and their applications in various sectors of the food, beverage and nutraceutical industries. Areas covered include dairy and egg processing, oilseed extraction, and brewing. This section discusses the characteristics of different foods and fluids, how food constituents are affected by separation processes and how separation processes can be designed and operated to optimize end product quality.With its team of experienced international contributors, Separation, extraction and concentration processes in the food, beverage and nutraceutical industries is an important reference source for professionals concerned with the development and optimisation of these processes. - Describes the latest advances in separation, extraction and concentration techniques and their applications in various sectors of the food, beverage and nutraceutical industries - Reviews emerging techniques of particular interest, such as pervaporation and pressurised liquid extraction - Explores the characteristics of different foods and fluids and how food constituents are affected by separation processes
| Author | : Jan Rydberg |
| Publisher | : CRC Press |
| Total Pages | : 760 |
| Release | : 2004-03-01 |
| Genre | : Science |
| ISBN | : 0824752201 |
A complete and up-to-date presentation of the fundamental theoretical principles and many applications of solvent extraction, this enhanced Solvent Extraction Principles and Practice, Second Edition includes new coverage of the recent developments in solvent extraction processes, the use of solvent extraction in analytical applications and waste recovery, and computational chemistry methods for modeling the solvent extraction of metal ions. Offering sound scientific and technical descriptions in a format accessible to students and expedient for researchers and engineers, this edition also features a new chapter on ionic strength corrections and contains more than 850 up-to-date literature citations.
| Author | : Paolo Nannipieri |
| Publisher | : Springer Science & Business Media |
| Total Pages | : 468 |
| Release | : 2006-09-22 |
| Genre | : Science |
| ISBN | : 354029449X |
With millions of different bacterial species living in soil, the microbial community is extremely complex, varying at very small scales. Microbe-driven functions are essential for most processes in soil. Thus, a better understanding of this microbial diversity will be invaluable for the management of the various soil functions. Nucleic Acids and Proteins in Soil combines traditional approaches in soil microbiology and biochemistry with the latest techniques in molecular microbial ecology. Included are methods to analyse the presence and importance of nucleic acids and proteins both inside and outside microbial cells, the horizontal gene transfer which drives bacterial diversity, as well as soil proteomes. Further chapters describe techniques such as PCR, fingerprinting, the challenging use of gene arrays for structural and functional analysis, stable isotope probing to identify in situ metabolic functions, and the use of marker and reporter genes in soil microbial ecology.
