Separation Methods In Proteomics

Separation Methods In Proteomics
Author: Gary B. Smejkal
Publisher: CRC Press
Total Pages: 532
Release: 2005-12-12
Genre: Medical
ISBN: 1420028057

Driven by the widespread growth of proteomic practices, protein separation techniques have been refined to minimize variability, optimize particular applications, and adapt to user preferences in the analysis of proteins. Separation Methods in Proteomics provides a comprehensive examination of all major separation techniques for proteomic

Separation Techniques Applied to Omics Sciences

Separation Techniques Applied to Omics Sciences
Author: Ana Valéria Colnaghi Simionato
Publisher: Springer Nature
Total Pages: 277
Release: 2021-10-09
Genre: Science
ISBN: 3030772527

This book covers liquid chromatography, gas chromatography and capillary electrophoresis, the three main separation techniques lately available, applied to key omic sciences, such as genomics, proteomics, metabolomics and foodomics. The fundamentals of each technique are not covered herein. Instead, the recent advances in such techniques are presented focusing on the application to omics analyses and unique aspects in each case. This volume intends to offer wide ranging options available to researchers on omics sciences, and how to integrate them in order to achieve the comprehension of a biological system as a whole. Omic sciences have been of ultimate importance to comprehend the complex biochemical reactions and related events that occurs upon a biological system. The classical central dogma of molecular biology, which states that genetic information flows unidirectionally from DNA to RNA and then to proteins, has been gradually replaced by the systems biology approach. This book presents a multidisciplinary approach that explains the biological system as a whole, where the entire organism is influenced by a variety of internal events as well as by the environment, showing that each level of the biological information flux may influence the previous or the subsequent one.

New Techniques for Proteomics Study: Instrumentation, Separation, and Application

New Techniques for Proteomics Study: Instrumentation, Separation, and Application
Author: Si Wu
Publisher:
Total Pages: 222
Release: 2006
Genre:
ISBN: 9781109906714

Second, to increase the mass measurement accuracy and protein identification confidence in FTICR-MS, we have developed a new calibration method, Calibration Optimization on Fragment Ions (COFI), for fragmentation data to correct the effects of space charge in FTICR-MS. There is a post-data processing strategy, and no need to include an internal calibrant or to apply ion population control. COFI has been successfully applied to different types of LC-FTICR fragmentation data, including LC-MS/MS data and multiplexed LC-CID data.

Spectral Techniques In Proteomics

Spectral Techniques In Proteomics
Author: Daniel S. Sem
Publisher: CRC Press
Total Pages: 464
Release: 2007-03-30
Genre: Medical
ISBN: 1420017098

Facilitating the innovation, development, and application of new spectroscopic methods in proteomics, Spectral Techniques in Proteomics provides a broad overview of the spectroscopic toolbox that can be used, either with proteome or sub-proteome mixtures or with individual/purified proteins studied in parallel. It gives a modest overview of

Proteomics Today

Proteomics Today
Author: Mahmoud H. Hamdan
Publisher: John Wiley & Sons
Total Pages: 448
Release: 2005-05-13
Genre: Science
ISBN: 0471709107

The last few years have seen an unprecedented drive toward theapplication of proteomics to resolving challenging biomedical andbiochemical tasks. Separation techniques combined with modern massspectrometry are playing a central role in this drive. This bookdiscusses the increasingly important role of mass spectrometry inproteomic research, and emphasizes recent advances in the existingtechnology and describes the advantages and pitfalls as well. * Provides a scientifically valid method for analyzing theapproximatey 500,000 proteins that are encoded in the humangenome * Explains the hows and whys of using mass spectrometry inproteomic analysis * Brings together the latest approaches combining separationtechniques and mass spectrometry and their application in proteomeanalysis * Comments on future challenges and how they may be addressed * Includes sections on troubleshooting

Introducing Proteomics

Introducing Proteomics
Author: Josip Lovric
Publisher: John Wiley & Sons
Total Pages: 310
Release: 2011-06-17
Genre: Science
ISBN: 1119957192

Introducing Proteomics gives a concise and coherent overview of every aspect of current proteomics technology, which is a rapidly developing field that is having a major impact within the life and medical sciences. This student-friendly book, based on a successful course developed by the author, provides its readers with sufficient theoretical background to be able to plan, prepare, and analyze a proteomics study. The text covers the following: Separation Technologies Analysis of Peptides/Proteins by Mass Spectrometry Strategies in Proteomics This contemporary text also includes numerous examples and explanations for why particular strategies are better than others for certain applications. In addition, Introducing Proteomics includes extensive references and a list of relevant proteomics information sources; essential for any student. This no-nonsense approach to the subject tells students exactly what they need to know, leaving out unnecessary information. The student companion site enhances learning and provides answers to the end of chapter problems. "I think this book will be a popular and valuable resource for students and newcomers to the field who would like to have an overview and initial understanding of what proteomics is about. The contents are well organized and address the major issues." —Professor Walter Kolch, Director, Systems Biology Ireland & Conway Institute, University College Dublin Companion Website www.wiley.com/go/lovric

Neuroproteomics

Neuroproteomics
Author: Oscar Alzate
Publisher: CRC Press
Total Pages: 356
Release: 2009-10-26
Genre: Medical
ISBN: 1420076264

In this, the post-genomic age, our knowledge of biological systems continues to expand and progress. As the research becomes more focused, so too does the data. Genomic research progresses to proteomics and brings us to a deeper understanding of the behavior and function of protein clusters. And now proteomics gives way to neuroproteomics as we beg

2D PAGE: Sample Preparation and Fractionation

2D PAGE: Sample Preparation and Fractionation
Author: Anton Posch
Publisher: Methods in Molecular Biology
Total Pages: 368
Release: 2008-03-19
Genre: Science
ISBN:

Focusing on two-dimensional electrophoresis (2-DE), this book sets forth the principles and recent developments of sample preparation and fractionation tools in expression proteomics. Step-by-step instructions alongside detailed illustrations enable you to quickly learn how to perform the methods yourself. Tricks of the trade and notes on troubleshooting and avoiding pitfalls help you ensure successful results every time. The methods covered in this book are not limited to 2-DE; they can also be applied to a broad range of protein separation techniques.

Proteomics in Foods

Proteomics in Foods
Author: Fidel Toldrá
Publisher: Springer Science & Business Media
Total Pages: 589
Release: 2012-12-16
Genre: Technology & Engineering
ISBN: 1461456266

Food proteomics is one of the most dynamic and fast-developing areas in food science. The goal of this book is to be a reference guide on the principles and the current and future potential applications of proteomics in food science and technology. More specifically, the book will discuss recent developments and the expected trends of the near future in food proteomics. The book will be divided into two parts. The first part (7 chapters) will focus on the basic principles for proteomics, e.g., sample preparation, such as extraction and separation techniques, analytical instrumentation currently in use, and available databases for peptide and protein identification. The second part of the book (26 chapters) will focus on applications in foods. It will deal with quality issues related to post-mortem processes in animal foods and quality traits for all foods in general, as well as the identification of bioactive peptides and proteins, which are very important from the nutritional point of view. Furthermore, consumers are now extremely susceptible to food safety issues, and proteomics can provide reassurance with different safety aspects, such as food authenticity, detection of animal species in the food, and identification of food allergens. All of these issues will be covered in this book. It is also worth noting that both editors are internationally recognized experts in the field of food science, and both have edited numerous food science books and handbooks.