Regulatory Mechanisms Of Chemosensory Signaling In Caenorhabditis Elegans
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Author | : Michelle Christine Krzyzanowski |
Publisher | : |
Total Pages | : 213 |
Release | : 2015 |
Genre | : |
ISBN | : |
All animals rely on their ability to sense and respond to their environment to survive. While similar signal transduction pathways are implicated in both C. elegans and vertebrate chemosensation, there are still large gaps in our understanding of the mechanisms used to regulate signaling in these systems. In my thesis, I have identified a new role for the C. elegans cGMP-dependent protein kinase (PKG) EGL-4 in the negative regulation of nociceptive chemosensory signaling. Nociceptive sensory systems detect harmful stimuli and allow for the initiation of protective behavioral responses. The polymodal ASH sensory neurons are the primary nociceptors in C. elegans. The data suggest that EGL-4 negatively regulates signaling and behavior by activating known inhibitors of G protein-coupled signal transduction, RGS proteins. Using C. elegans behavioral response to aversive stimuli as the readout for neuronal activity, I provide the first evidence for PKG regulation of RGS function in sensory neurons in any system. Additionally, the suitability of a behavioral response is context-dependent, and must reflect both an animal's life history and its present internal state. Based on the integration of these variables, an animal's needs can be prioritized to optimize survival strategies. I show that cGMP movement through a gap junction neural network allows dynamic repurposing of several C. elegans head sensory neurons to regulate ASH sensitivity through EGL-4 function in response to an animal's feeding status. Such decentralized regulation of ASH signaling allows for rapid correlation between an animal's internal state and its behavioral output, and lends an unexpected modulatory flexibility to this hard-wired nociceptive neural circuit.
Author | : Meredith Jean Ezak |
Publisher | : |
Total Pages | : 121 |
Release | : 2011 |
Genre | : |
ISBN | : |
All animals rely on their ability to sense and respond to their constantly changing environments to survive. Caenorhabditis elegans depend heavily upon their ability to taste and smell chemical information in their soil habitat to find favorable conditions and avoid noxious environments. While similar signal transduction pathways are implicated in both C. elegans and vertebrate chemosensation, there are still large gaps in our understanding of the mechanisms used to regulate signaling in these systems. I have sought to define cellular pathways that modulate signaling activity, and ultimately responsiveness, of chemosensory neurons in C. elegans. I describe herein the identification of cell- and modality-specific pathways that regulate neuronal activity in response to aberrant signaling, as well as a novel mechanism by which neuronal cells may regulate expression of sensory genes to directly impact cellular activity.
Author | : Eva B. Nokes |
Publisher | : |
Total Pages | : 93 |
Release | : 2010 |
Genre | : Caenorhabditis elegans |
ISBN | : |
Author | : Anna Menini |
Publisher | : CRC Press |
Total Pages | : 438 |
Release | : 2009-11-24 |
Genre | : Science |
ISBN | : 1420071998 |
Comprehensive Overview of Advances in OlfactionThe common belief is that human smell perception is much reduced compared with other mammals, so that whatever abilities are uncovered and investigated in animal research would have little significance for humans. However, new evidence from a variety of sources indicates this traditional view is likely
Author | : Leigh R. Wexler |
Publisher | : |
Total Pages | : 126 |
Release | : 2019 |
Genre | : |
ISBN | : |
The feeding-state of an animal can have deep effects on its chemosensation and behavioral decisions. Although many internal signals of nutritional state and hunger are known, the mechanisms by which these modulate neural circuits and behavior are less well understood. C. elegans display an interesting intersection of feeding state and sex in the regulation of chemosensory behavior. Self-fertile hermaphrodites exhibit strong food attraction while males prioritize mate-searching over feeding. This sexual dimorphism is regulated in part through differences the chemoreceptor ODR-10, which detects the food-associated odorant diacetyl in the AWA neuron. Hermaphrodites expresses high levels of odr-10 while in adult males, expression of odr-10 is low, promoting exploratory behavior. However, starved males transiently upregulate odr-10 to generate stronger food attraction until their nutritional needs are met. This multi-layered regulation of odr-10 provides an opportunity to study how neural circuits integrate genetic sex and feeding-state to regulate chemosensory behaviors through chemoreceptor expression. We show that in response to food availability, odr-10 is modulated in males through insulin-like signaling and DAF-7/TGF? signaling. Interestingly, we find that in males odr-10 is regulated primarily by sensory perception of food, not metabolic starvation. Similarly, the TGF? ligand daf-7 is regulated in the ASJ neuron by food perception, linking environmental signals to odr-10. Additionally, these mechanisms are modulated by sex. We show hermaphrodites also regulate odr-10 in response to feeding state, however they do so through divergent mechanisms from males, independent of insulin and TFG? signaling and in response to metabolic starvation. Through this work we identify a male-specific mechanisms which modulates chemosensation based on environmental conditions furthering our understanding of how chemoreceptor expression and behavioral choice is regulated through multiple internal and external states.
Author | : Howard C. Berg |
Publisher | : Springer Science & Business Media |
Total Pages | : 136 |
Release | : 2008-01-11 |
Genre | : Science |
ISBN | : 0387216383 |
Escherichia coli, commonly referred to as E. coli, has been the organism of choice for molecular genetics for decades. Its machinery and mobile behavior is one of the most fascinating topics for cell scientists. Scientists and engineers, not trained in microbiology, and who would like to learn more about living machines, can see it as a unique example. This cross-disciplinary monograph covers more than thirty years of research and is accessible to graduate students and scientists alike.
Author | : Donald L. Riddle |
Publisher | : Firefly Books |
Total Pages | : 1252 |
Release | : 1997 |
Genre | : Medical |
ISBN | : 9780879695323 |
Defines the current status of research in the genetics, anatomy, and development of the nematode C. elegans, providing a detailed molecular explanation of how development is regulated and how the nervous system specifies varied aspects of behavior. Contains sections on the genome, development, neural networks and behavior, and life history and evolution. Appendices offer genetic nomenclature, a list of laboratory strain and allele designations, skeleton genetic maps, a list of characterized genes, a table of neurotransmitter assignments for specific neurons, and information on codon usage. Includes bandw photos. For researchers in worm studies, as well as the wider community of researchers in cell and molecular biology. Annotation copyrighted by Book News, Inc., Portland, OR
Author | : Manon Guillermin |
Publisher | : |
Total Pages | : 97 |
Release | : 2018 |
Genre | : |
ISBN | : |
Adaptability is essential to organisms' fitness and survival. Evolutionary success depends on access to an array of behavioral choices in the face of changing environmental conditions. To navigate complex landscapes, organisms can interpret the significance of sensory stimuli, and assign context-appropriate valence, by integrating factors such as cues from their internal and external environments, and memories of previously experienced conditions, to dynamically shape neural circuits and generate ethologically relevant behaviors. In this thesis, I explore the cellular and molecular mechanisms that shape the carbon dioxide (CO2) circuit in the free-living nematode, Caenorhabditis elegans. CO2 is a complex sensory cue that can signify the presence of fruitful or dangerous surroundings. As a result, C. elegans can display a variety of different behaviors in response to CO2, from robust attraction to robust avoidance. Although sensory signaling of the CO2-responsive BAG neurons has been extensively characterized, how BAG communicates with postsynaptic interneurons, and how the CO2 signal is propagated through the nervous system to generate a context-appropriate behavior is unknown. First, we have found that neuromodulatory state and environmental oxygen (O2) levels converge on the CO2 circuit via the URX sensory neurons. The lab-derived N2 C. elegans strain expresses high levels of NPR-1 neuropeptide receptor, which inhibits URX and results in CO2 avoidance, regardless of environmental O2. In the C. elegans wild isolate "Hawaii", loss of npr-1 leads to modulation of URX by environmental O2, and results in CO2 avoidance at low O2, and loss of CO2-evoked behavior at high O2. Second, we present a new circuit motif that demonstrates how divergent responses to a single sensory input, CO2, can arise from an identical set of sensory and interneuron connections. We show that C. elegans exhibit an experience-dependent behavioral valence switch in response to CO2. While animals raised at ambient CO2 are repelled by CO2, animals raised in a high CO2 environment are attracted to CO2. Whether CO2 is attractive or repulsive is determined by the coordinated activity of specialized valence-encoding interneurons, AIY, RIG, and RIA, whose responses are subject to context-dependent modulation. An additional interneuron pair, AIZ, regulates behavioral sensitivity regardless of valence. Glutamatergic and neuropeptidergic signaling mediate both CO2 avoidance and attraction, and different neuropeptides play distinct roles in regulating valence and sensitivity. Our results elucidate a microcircuit motif whereby a fixed set of neurons are leveraged to generate alternative outputs in response to a single chemosensory input.
Author | : Eric H. Davidson |
Publisher | : Elsevier |
Total Pages | : 274 |
Release | : 2001-01-24 |
Genre | : Science |
ISBN | : 0080525598 |
The interaction between biology and evolution has been the subject of great interest in recent years. Because evolution is such a highly debated topic, a biologically oriented discussion will appeal not only to scientists and biologists but also to the interested lay person. This topic will always be a subject of controversy and therefore any breaking information regarding it is of great interest.The author is a recognized expert in the field of developmental biology and has been instrumental in elucidating the relationship between biology and evolution. The study of evolution is of interest to many different kinds of people and Genomic Regulatory Systems: In Development and Evolution is written at a level that is very easy to read and understand even for the nonscientist. * Contents Include* Regulatory Hardwiring: A Brief Overview of the Genomic Control Apparatus and Its Causal Role in Development and Evolution * Inside the Cis-Regulatory Module: Control Logic and How the Regulatory Environment Is Transduced into Spatial Patterns of Gene Expression* Regulation of Direct Cell-Type Specification in Early Development* The Secret of the Bilaterians: Abstract Regulatory Design in Building Adult Body Parts* Changes That Make New Forms: Gene Regulatory Systems and the Evolution of Body Plans
Author | : T.Y. Chang |
Publisher | : Springer Science & Business Media |
Total Pages | : 298 |
Release | : 2012-12-06 |
Genre | : Science |
ISBN | : 1461551137 |
INTRODUCTION AND RATIONALE FOR INTRACELLULAR CHOLESTEROL TRAFFICKING This volume is an elaboration of an earlier small meeting held in St. Louis, Missouri. In April 1997, many of the authors met for a two-day meeting devoted entirely to intracellular cholesterol trafficking. The rationale for this meeting was that investigators interested in this topic worked in a variety of fields, and rarely, if ever, all met together. Everybody knew each other's papers but mostly worked in isolation from one another. Understanding of cholesterol trafficking also appeared to have reached the point where it would start to rapidly expand beyond these few laboratories. Understanding of cholesterol trafficking was moving from a largely descriptive science into the molecular age. It seemed a good time to get together and see how much we agreed upon up to this point. More authors contributed to this volume than attended the St. Louis meeting. That meeting was generously funded by grants from Bristol-Myers Squibb, Merck and Company and Parke-Davis, however, the total funding available limited the size of the meeting. For the book, we are not so limited and have tried to be as inclusive as possible and pretty much invited everyone who is presently active in this area. We were quite fortunate to successfully recruit the authors we sought for each of these chapters. The authors and their contributions can be organized by particular interests and particular areas of expertise.