Radioimmunoassay
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| Author | : Carlo Patrono |
| Publisher | : Springer Science & Business Media |
| Total Pages | : 625 |
| Release | : 2012-12-06 |
| Genre | : Medical |
| ISBN | : 3642718094 |
Thirty years have elapsed since the first description by S. A. BERSON and R. S. Y ALOW of the basic principles of radioimmunoassay (RIA). During this period of time, RIA methodology has been instrumental to the growth of many areas of biomedical research, including endocrinology, oncology, hematology, and pharmacology. It has done so by providing a relatively simple universal tool allowing, for the first time, the detection of endogenous mediators that are present 12 10 in body fluids at concentrations as low as 10- _10- M. The fundamental nature of this discovery and the wide-ranging fall-out of basic and clinical knowledge derived from its application have been acknowledged by the many honors tributed to its pioneers, including the Nobel Prize awarded to Dr. Y ALOW 10 years ago. Although several excellent books have been published during the past decades covering various aspects of RIA methodology, we felt the need, as pharmacologists, for a comprehensive discussion of the methodological and conceptual issues related to the main classes of mediators of drug action and to drugs themselves. Thus, we gladly accepted the challenge provided by the invitation to edit a volume of the Handbook of Experimental Pharmacology on Radioimmunoassay in Basic and Clinical Pharmacology. We tried to balance the emphasis placed on more general aspects of the RIA methodology and that on specific mediators.
| Author | : Vijay Kumar |
| Publisher | : Springer |
| Total Pages | : 174 |
| Release | : 2018-03-30 |
| Genre | : Medical |
| ISBN | : 9811081867 |
This book is a practical guidebook in biochemistry, for medical as well as life sciences' students. The book covers reference values, sample collection procedure and detailed protocol to perform experiments. Each experiment starts with a brief introduction of the protocol, followed by specimen requirements and procedure. The procedures are presented in a very lucid manner and discuss details of calculations and clinical interpretations,The book is divided into 29 chapters, It offers references, general guidelines and abbreviations and provides principles and procedures of clinical biochemistry tests, along with their diagnostic importance.
| Author | : Rowa Yousef Alhabbab |
| Publisher | : Springer |
| Total Pages | : 144 |
| Release | : 2018-05-23 |
| Genre | : Medical |
| ISBN | : 3319776940 |
This book discusses in detail various serological tests that are used to identify medical conditions and diseases, from a general overview of each test to the equipment and steps needed to carry them out. The book is aimed specifically at immunology students and professionals who may occasionally need to use these tests, and thus lack training and experience in performing them. The book provides a brief overview of the immune system, including antibodies, antigens, and their interactions. The bulk of the book is comprised of 16 chapters that each explain different serological tests. These chapters start with a general introduction of the test or disease being detected, followed by the test principle, reagents required for the test, procedures and steps to perform the test, and, finally, result interpretation. Both test principles and result interpretation segments include illustrations to aid comprehension. In addition, the book also enables the reader to distinguish between positive and negative results in serological testing.
| Author | : Bernard Jaffe |
| Publisher | : Elsevier |
| Total Pages | : 1071 |
| Release | : 2012-12-02 |
| Genre | : Science |
| ISBN | : 032316126X |
Methods of Hormone Radioimmunoassay, Second Edition is a compilation of radioimmunoassay techniques for the quantification of hormones. Various kinds of hormones are considered, namely: intracellular messengers; hypothalamic and pineal hormones; pituitary hormones; thyroid and parathyroid hormones; renal hormones; hormones of the gastrointestinal tract; pancreatic hormones; steroid hormones; uterine and placental hormones; vasoactive peptide hormones; and growth factors. The corresponding method of radioimmunoassay for each hormone is described. This book is comprised of 49 chapters and begins with a discussion on the radioimmunoassay of cyclic adenosine-3',5-monophosphate (cAMP) and cyclic guanosine-3,5'-monophosphate (cGMP), together with problems related to measuring both compounds. The reader is then introduced to prostaglandins and prostaglandin metabolites, thyrotropin-releasing hormone, and gonadotropin-releasing hormone. Pituitaty gonadotropins, prolactin, and thyrotropin are also discussed. The remaining chapters deal with hormones of the gastrointestinal tract, pancreatic hormones, steroid hormones, and growth factors. This monograph is intended for both clinicians and investigators interested in methods of hormone radioimmunoassay.
| Author | : Eleftherios P. Diamandis |
| Publisher | : Academic Press |
| Total Pages | : 613 |
| Release | : 1996-06-21 |
| Genre | : Medical |
| ISBN | : 0080534503 |
Immunoassays are among the most powerful and sensitive technologies now available for patient diagnosis and monitoring. This book is an indispensable guide to information on the theory and practice of immunoassays. It discusses the scientific basis of these technologies in a logical, organized, and heuristic manner and provides protocols for specific assays. The contents of this unique book are balanced among theory, practical issues, quality control, automation, and subspecialty areas, making it ideal for health science students, laboratory scientists, and clinicians. - Presents up-to-date information - Provides extensive cross-referencing - Covers theory and practice in full detail - Written by leading authorities
| Author | : Lemuel J. Bowie |
| Publisher | : CRC Press |
| Total Pages | : 305 |
| Release | : 2018-02-06 |
| Genre | : Science |
| ISBN | : 135108688X |
The various instruments described in this text all provide an integrated and automated approach for separating free and bound ligands along with other steps in radioimmunoassay. Although the various systems differ significantly in design, they all perform these basic manoeuvres, for the most part, without any operator intervention. It is hoped that the following descriptions of the various system capabilities will not only provide useful information for present and potential users of the systems, but also stimulate additional development by manufacturers which will bring the full potential of radioimmunoassay even closer to a reality.
| Author | : Ivor L. Simmons |
| Publisher | : Springer Science & Business Media |
| Total Pages | : 188 |
| Release | : 2013-11-11 |
| Genre | : Science |
| ISBN | : 1468433210 |
| Author | : Y. Pomeranz |
| Publisher | : Springer Science & Business Media |
| Total Pages | : 788 |
| Release | : 2013-12-01 |
| Genre | : Technology & Engineering |
| ISBN | : 1461569982 |
The first edition of Food Analysis: Theory and Practice was published in 1971 and was revised in 1978. The second edition was published in 1987, and in 1993 we found it necessary to prepare a third edition to reflect and cover the most recent advances in the field of food analysis. A complete revision of a book is an arduous and anguished task. The following are challenges that we wanted to address in this revision: to update the material without eliminating classic and time-preserved and honored methods used by the food analyst; to broaden and deepen the coverage and scope without increasing the size of the book; and to produce a textbook (for senior undergraduate and graduate students) with regard to objectives, scope, and outlay while providing a reference and resource for the worker and researcher in the field of food analysis. To meet those challenges we added much new material and took out practically the same amount of "rel atively outdated" material. Every chapter has been extensively updated and revised; many of the pictures in the previous editions were deleted and, whenever available and appropriate, were replaced by diagrams or flow sheets. In Part I we have expanded the seetions on sampling, preparation of sam pIes, reporting results, and reliability of analyses.
| Author | : Eric Reid |
| Publisher | : Springer Science & Business Media |
| Total Pages | : 407 |
| Release | : 2013-03-12 |
| Genre | : Science |
| ISBN | : 1461323975 |
Acknowledgements. - Valuable support for the Forum came from the Cancer Research Campaign, from Johnson Matthey & Co., and from U. K. pharmaceutical companies - Beechams, Glaxo, ICI and Smith, Kline & French. Moreover, some speakers came without full financial coverage. The choice of presentations was guided by Honorary Advisers including Drs. S.H. Curry (Chairman), J.A.F. de Silva, L.E. Martin, J. Chamberlain and G.G. Skellern. Drs. Jim Leppard and Joan Reid are thanked for Index drafting. As mentioned in the text, some Figs. have already appeared in journals, whose publishers (e.g. Elsevier, Dekker, Preston) are thanked: sources include Journal of Chroma- tography, Journal of Liquid Chromatography and Journal of Chromatographic Science, also (art. #E-S) a Wiley book edited by M. Trimble. Abbreviations.- In connection with HPLC ('LC' is a pet aver- sion) this Editor has often deplored the upstart use of 'ECD'-a term hallowed by its GC usage as in art. #F -2 later in the book. To connote 'electrochemical' the term 'EC' is now used, but 'ECD' is reserved for the electron-capture detector.Other abbreviations which, although well known, are generally defined in each article concerned include NP, normal-phase HPLC); RP, reverse(d)-phase; i.s., internal standard; MS, mass spectrometry (EI, electron-impact; CI, chemic, al-ionization); RIA, radioimmunoassay; UV, ultraviolet (usually absorbance)
| Author | : David Wild |
| Publisher | : Newnes |
| Total Pages | : 1037 |
| Release | : 2013-01-21 |
| Genre | : Science |
| ISBN | : 0080970389 |
The fourth edition of The Immunoassay Handbook provides an excellent, thoroughly updated guide to the science, technology and applications of ELISA and other immunoassays, including a wealth of practical advice. It encompasses a wide range of methods and gives an insight into the latest developments and applications in clinical and veterinary practice and in pharmaceutical and life science research. Highly illustrated and clearly written, this award-winning reference work provides an excellent guide to this fast-growing field. Revised and extensively updated, with over 30% new material and 77 chapters, it reveals the underlying common principles and simplifies an abundance of innovation. The Immunoassay Handbook reviews a wide range of topics, now including lateral flow, microsphere multiplex assays, immunohistochemistry, practical ELISA development, assay interferences, pharmaceutical applications, qualitative immunoassays, antibody detection and lab-on-a-chip. This handbook is a must-read for all who use immunoassay as a tool, including clinicians, clinical and veterinary chemists, biochemists, food technologists, environmental scientists, and students and researchers in medicine, immunology and proteomics. It is an essential reference for the immunoassay industry. Provides an excellent revised guide to this commercially highly successful technology in diagnostics and research, from consumer home pregnancy kits to AIDS testing.www.immunoassayhandbook.com is a great resource that we put a lot of effort into. The content is designed to encourage purchases of single chapters or the entire book. David Wild is a healthcare industry veteran, with experience in biotechnology, pharmaceuticals, medical devices and immunodiagnostics, which remains his passion. He worked for Amersham, Eastman-Kodak, Johnson & Johnson, and Bristol-Myers Squibb, and consulted for diagnostics and biotechnology companies. He led research and development programs, design and construction of chemical and biotechnology plants, and integration of acquired companies. Director-level positions included Research and Development, Design Engineering, Operations and Strategy, for billion dollar businesses. He retired from full-time work in 2012 to focus on his role as Editor of The Immunoassay Handbook, and advises on product development, manufacturing and marketing. - Provides a unique mix of theory, practical advice and applications, with numerous examples - Offers explanations of technologies under development and practical insider tips that are sometimes omitted from scientific papers - Includes a comprehensive troubleshooting guide, useful for solving problems and improving assay performancee - Provides valuable chapter updates, now available on www.immunoassayhandbook.com
