Quantitative Phase Imaging

Quantitative Phase Imaging
Author: Gabriel Popescu
Publisher:
Total Pages:
Release: 2015
Genre: Diagnostic imaging
ISBN:

'Proceedings of SPIE' presents the original research papers presented at SPIE conferences and other high-quality conferences in the broad-ranging fields of optics and photonics. These books provide prompt access to the latest innovations in research and technology in their respective fields.

Quantitative Phase Imaging of Cells and Tissues

Quantitative Phase Imaging of Cells and Tissues
Author: Gabriel Popescu
Publisher: McGraw Hill Professional
Total Pages: 384
Release: 2011-03-14
Genre: Technology & Engineering
ISBN: 0071663436

Cutting-edge quantitative phase imaging techniques and their applications Filled with unique, full-color images taken by advanced quantitative phase imaging (QPI), Quantitative Phase Imaging of Cells and Tissues thoroughly explores this innovative technology and its biomedical applications. An introductory background on optical imaging and traditional optical microscopy is included to illustrate concept development. The book explains how various visualization modalities can be obtained by numerical calculations. This authoritative resource reveals how to take full advantage of the unprecedented capabilities of QPI, such as rendering scattering properties of minute subcellular structures and nanoscale fluctuations in live cells. Coverage includes: Groundwork Spatiotemporal field correlations Image characteristics Light microscopy Holography Point scanning QPI methods Principles of full-field QPI Off-axis full-field methods Phase-shifting techniques Common-path methods White light techniques Fourier transform light scattering (FTLS) Current trends in QPI

Coherent Light Microscopy

Coherent Light Microscopy
Author: Pietro Ferraro
Publisher: Springer Science & Business Media
Total Pages: 378
Release: 2011-02-09
Genre: Science
ISBN: 3642158137

This book deals with the latest achievements in the field of optical coherent microscopy. While many other books exist on microscopy and imaging, this book provides a unique resource dedicated solely to this subject. Similarly, many books describe applications of holography, interferometry and speckle to metrology but do not focus on their use for microscopy. The coherent light microscopy reference provided here does not focus on the experimental mechanics of such techniques but instead is meant to provide a users manual to illustrate the strengths and capabilities of developing techniques. The areas of application of this technique are in biomedicine, medicine, life sciences, nanotechnology and materials sciences.

Methods in Nano Cell Biology

Methods in Nano Cell Biology
Author:
Publisher: Academic Press
Total Pages: 534
Release: 2008-12-11
Genre: Science
ISBN: 008094910X

Understanding live cells at the single molecule level is the most important and single major challenge facing biology and medicine today. Nanobiology focuses on the properties and structure of complex assemblies of biomolecules—biochips and molecular motors, for example—in conjunction with distinctive surfaces, rods, dots, and materials of nanoscience. Nano Cell Biology will describe the current applications of nanobiology to the study of the structure, function, and metabolic processes of cells. - Provides historical background on this newly emerging field - Covers the latest application of new instrumentation in the field - Detailed protocols in the study of live cells at the nanometer level - Discusses future technologies and their applications in the study of living cells

Quantitative Imaging in Cell Biology

Quantitative Imaging in Cell Biology
Author:
Publisher: Academic Press
Total Pages: 609
Release: 2014-06-25
Genre: Science
ISBN: 0124202012

This new volume, number 123, of Methods in Cell Biology looks at methods for quantitative imaging in cell biology. It covers both theoretical and practical aspects of using optical fluorescence microscopy and image analysis techniques for quantitative applications. The introductory chapters cover fundamental concepts and techniques important for obtaining accurate and precise quantitative data from imaging systems. These chapters address how choice of microscope, fluorophores, and digital detector impact the quality of quantitative data, and include step-by-step protocols for capturing and analyzing quantitative images. Common quantitative applications, including co-localization, ratiometric imaging, and counting molecules, are covered in detail. Practical chapters cover topics critical to getting the most out of your imaging system, from microscope maintenance to creating standardized samples for measuring resolution. Later chapters cover recent advances in quantitative imaging techniques, including super-resolution and light sheet microscopy. With cutting-edge material, this comprehensive collection is intended to guide researchers for years to come. Covers sections on model systems and functional studies, imaging-based approaches and emerging studies Chapters are written by experts in the field Cutting-edge material

Label-Free Super-Resolution Microscopy

Label-Free Super-Resolution Microscopy
Author: Vasily Astratov
Publisher: Springer Nature
Total Pages: 498
Release: 2019-08-31
Genre: Science
ISBN: 3030217221

This book presents the advances in super-resolution microscopy in physics and biomedical optics for nanoscale imaging. In the last decade, super-resolved fluorescence imaging has opened new horizons in improving the resolution of optical microscopes far beyond the classical diffraction limit, leading to the Nobel Prize in Chemistry in 2014. This book represents the first comprehensive review of a different type of super-resolved microscopy, which does not rely on using fluorescent markers. Such label-free super-resolution microscopy enables potentially even broader applications in life sciences and nanoscale imaging, but is much more challenging and it is based on different physical concepts and approaches. A unique feature of this book is that it combines insights into mechanisms of label-free super-resolution with a vast range of applications from fast imaging of living cells to inorganic nanostructures. This book can be used by researchers in biological and medical physics. Due to its logically organizational structure, it can be also used as a teaching tool in graduate and upper-division undergraduate-level courses devoted to super-resolved microscopy, nanoscale imaging, microscopy instrumentation, and biomedical imaging.

Imaging Cellular and Molecular Biological Functions

Imaging Cellular and Molecular Biological Functions
Author: Spencer L. Shorte
Publisher: Springer Science & Business Media
Total Pages: 457
Release: 2007-09-12
Genre: Science
ISBN: 354071331X

This book offers a comprehensive selection of essays by leading experts, which covers all aspects of modern imaging, from its application and up-scaling to its development. The chapter content ranges from the basics to the most complex overview of method and protocols. There is ample practical and detailed "how-to" content on important, but rarely addressed topics. This first edition features all-colour-plate chapters, licensed software and a unique, continuously updated website forum.

Video Microscopy

Video Microscopy
Author: Leslie Wilson
Publisher: Gulf Professional Publishing
Total Pages: 362
Release: 1998-02-26
Genre: Computers
ISBN: 9780126491609

Video microscopy is used extensively in many life and biomedical science disciplines today, and is a useful tool for both cell biologists and students. This book presents how to track the dynamic changes that take place in the structure of living cells and in reconstituted preparations using video and digital imaging microscopy. Basic information, principles, and applications are also covered, as well as more specialized video microscopy techniques. Chapters cover the commonly used video technologies in biological research Nontechnical presentation of principles Emphasis on the practical aspects of instrument use Covers pitfalls in instrument uses that can lead to artifacts Authors are leaders in the design and application of video methods to biological microscopy Presentation of material tailored for the established researcher that has little experience with video methods Examples are extensively illustrated with photographs

Live Cell Imaging

Live Cell Imaging
Author: Robert D. Goldman
Publisher: CSHL Press
Total Pages: 658
Release: 2005
Genre: Science
ISBN: 9780879696832

Recent advances in imaging technology reveal, in real time and great detail, critical changes in living cells and organisms. This manual is a compendium of emerging techniques, organized into two parts: specific methods such as fluorescent labeling, and delivery and detection of labeled molecules in cells; and experimental approaches ranging from the detection of single molecules to the study of dynamic processes in organelles, organs, and whole animals. Although presented primarily as a laboratory manual, the book includes introductory and background material and could be used as a textbook in advanced courses. It also includes a DVD containing movies of living cells in action, created by investigators using the imaging techniques discussed in the book. The editors, David Spector and Robert Goldman, whose previous book was Cells: A Laboratory Manual,are highly respected investigators who have taught microscopy courses at Cold Spring Harbor Laboratory, the Marine Biology Laboratory at Woods Hole, and Northwestern University.