Protocols for Cyanobacteria Sampling and Detection of Cyanotoxin

Protocols for Cyanobacteria Sampling and Detection of Cyanotoxin
Author: N. Thajuddin
Publisher: Springer Nature
Total Pages: 504
Release: 2024-01-19
Genre: Science
ISBN: 9819945143

This protocol book provides detailed procedures for the isolation of cyanobacteria, extraction, quantification, and detection of cyanotoxins. It illustrates the sampling and processing of toxin-producing cyanobacteria in water and aquatic animal samples, detection of cyanotoxins from Anabaena, Anabaenopsis, Cylindrospermopsis, Microsystis, Microcystis, Nodularia, Nostoc, Schizotrix, Lyngbya, Raphidiopsis, Oscillatoria, Planktothrix in aquatic resources. It also covers toxicity analysis by various bioassay protocols, and in vitro and insilico analysis methods. The book also reviews the methods for cyanotoxin extraction, detection, and quantification by various tools including LC-MS/MS, HPLC, NMR, PCR, and HESI-MS/MS. A separate section is dedicated to the advanced methods in Cyanotoxin analysis including the Molecular Imprinting Method (MIM), Cellular signaling biosensor, Electrochemical sensor, Nanosensors, and screening of Polyketide synthase gene. The analysis of various toxin-producing genes like sxtA and mcy is also accounted for in this book. In a nutshell, the book gives comprehensive procedures about the basics and preliminary processes that are involved in sample collection to advanced methods incorporated into the well-explored and unexplored Cyanobacterial toxin. Consequently, this manual is useful for both beginners and advanced researchers, including postgraduate students, academicians, researchers, and scientists in the field of Cyanobacterial research. ​

Toxic Cyanobacteria in Water

Toxic Cyanobacteria in Water
Author: Ingrid Chorus
Publisher: CRC Press
Total Pages: 701
Release: 2021-03-08
Genre: Technology & Engineering
ISBN: 1000262049

Cyanobacterial toxins are among the hazardous substances most widely found in water. They occur naturally, but concentrations hazardous to human health are usually due to human activity. Therefore, to protect human health, managing lakes, reservoirs and rivers to prevent cyanobacterial blooms is critical. This second edition of Toxic Cyanobacteria in Water presents the current state of knowledge on the occurrence of cyanobacteria and cyanotoxins as well as their impacts on health through water-related exposure pathways, chiefly drinking-water and recreational activity. It provides scientific and technical background information to support hazard identification, assessment and prioritisation of the risks posed by cyanotoxins, and it outlines approaches for their management at each step of the water-use system. It sets out key practical considerations for developing management strategies, implementing efficient measures and designing monitoring programmes. This enables stakeholders to evaluate whether there is a health risk from toxic cyanobacteria and to mitigate it with appropriate measures. This book is intended for those working on toxic cyanobacteria with a specific focus on public health protection. It intends to empower professionals from different disciplines to communicate and cooperate for sustainable management of toxic cyanobacteria, including public health workers, ecologists, academics, and catchment and waterbody managers. Ingrid Chorus headed the department for Drinking-Water and Swimming-Pool Hygiene at the German Environment Agency. Martin Welker is a limnologist and microbiologist, currently with bioMérieux in Lyon, France.

Molecular Tools for the Detection and Quantification of Toxigenic Cyanobacteria

Molecular Tools for the Detection and Quantification of Toxigenic Cyanobacteria
Author: Rainer Kurmayer
Publisher: John Wiley & Sons
Total Pages: 446
Release: 2017-09-05
Genre: Science
ISBN: 1119332109

A guide to state-of-the-art molecular tools for monitoring and managing the toxigenicity of cyanobacteria Runaway eutrophication and climate change has made the monitoring and management of toxigenic organisms in the world’s bodies of water more urgent than ever. In order to influence public policy regarding the detection and quantification of those organisms, it is incumbent upon scientists to raise the awareness of policy makers concerning the increased occurrence of toxigenic cyanobacteria and the threats they pose. As molecular methods can handle many samples in short time and help identify toxigenic organisms, they are reliable, cost-effective tools available for tracking toxigenic cyanobacteria worldwide. This volume arms scientists with the tools they need to track toxigenicity in surface waters and food supplies and, hopefully, to develop new techniques for managing the spread of toxic cyanobacteria. This handbook offers the first comprehensive treatment of molecular tools for monitoring toxigenic cyanobacteria. Growing out of the findings of the landmark European Cooperation in Science and Technology Cyanobacteria project (CYANOCOST), it provides detailed, practical coverage of the full array of available molecular tools and protocols, from water sampling, nucleic acid extraction, and downstream analysis—including PCR and qPCR based methods—to genotyping (DGGE), diagnostic microarrays, and community characterization using next-gen sequencing techniques. Offers an overview of the latest trends in the field, while providing a foundation for understanding and applying the tools and techniques described Provides detailed coverage of the full range of molecular tools currently available, with expert guidance on the analysis and interpretation of results Includes step-by-step guidance on standard operational procedures, including molecular tests used in environmental monitoring, with individual chapters devoted to each procedure Complements the published Handbook of Cyanobacterial Monitoring and Cyanotoxin Analysis from the CyanoCOST project This handbook is an indispensable working resource for scientists, lab technicians, and water management professionals and an excellent text/reference for graduate students and supervisors who use molecular tools. It will also be of great value to environmental health and protection officials and policy makers.

Cyanobacterial Harmful Algal Blooms: State of the Science and Research Needs

Cyanobacterial Harmful Algal Blooms: State of the Science and Research Needs
Author: H. Kenneth Hudnell
Publisher: Springer Science & Business Media
Total Pages: 955
Release: 2008-03-13
Genre: Medical
ISBN: 0387758658

With the ever-increasing incidence of harmful cyanobacterial algal blooms, this monograph has added urgency and will be essential reading for all sorts of researchers, from neuroscientists to cancer research specialists. The volume contains the proceedings of the 2005 International Symposium on Cyanobacterial Harmful Algal Blooms, and has been edited by H. Kenneth Hudnell, of the US Environmental Protection Agency. It contains much of the most recent research into the subject.

Algae Detection and Removal Strategies for Drinking Water Treatment Plants

Algae Detection and Removal Strategies for Drinking Water Treatment Plants
Author:
Publisher: American Water Works Association
Total Pages: 520
Release: 2004
Genre: Algae
ISBN: 9781583213070

This manual for conventional water treatment plants outlines monitoring strategies for detecting the onset of algae blooms in drinking water sources as well as treatment strategies for minimizing the adverse effects of algae on unit process performance and finished water quality. The manual draws on

Guidelines on recreational water quality. Volume 1

Guidelines on recreational water quality. Volume 1
Author: World Health Organization
Publisher: World Health Organization
Total Pages: 164
Release: 2021-07-12
Genre: Medical
ISBN: 9240031308

Use of coastal, estuarine and freshwater recreational environments has significant benefits for health and well-being, including rest, relaxation, exercise, cultural and religious practices, and aesthetic pleasure, while also providing substantial local, regional and national economic benefits. These guidelines focus on water quality management for coastal and freshwater environments to protect public health. The guidelines: 1. describe the current state of knowledge about the possible adverse health impacts of various forms of water pollution; and2. set out recommendations for setting national health-based targets, conducting surveillance and risk assessments, putting in place systems to monitor and control risks, and providing timely advice to users on water safety.These guidelines are aimed at national and local authorities, and other entities with an obligation to exercise due diligence relating to the safety of recreational water sites. They may be implemented in conjunction with other measures for water safety (such as drowning prevention and sun exposure) and measures for environmental protection of recreational water use sites.

Guidelines for Canadian Drinking Water Quality

Guidelines for Canadian Drinking Water Quality
Author:
Publisher:
Total Pages: 0
Release: 2023
Genre: Drinking water
ISBN: 9780660460239

"Malathion is a registered insecticide and acaricide used on a wide variety of sites including agricultural and non-agricultural sites. In 2018 (the most recent year for which data are available), over 25 000 kg of malathion was sold in Canada. Malathion may be released into surface water or soil as runoff from the application site. Malathion is not usually found in drinking water sources in Canada. Low levels of malathion have been found in several Canadian provinces. The maximum reported concentrations are well below the MAC. Malathion is rarely detected in foods"--Executive summary.

Toxic Microcystis

Toxic Microcystis
Author: Mariyo F. Watanabe
Publisher: CRC Press
Total Pages: 284
Release: 2024-11-01
Genre: Science
ISBN: 1040288367

This book discusses toxic Microcystis and the toxins from various viewpoints such as classification, cultivation, occurrences in lakes, and relations to zooplankton. The text presents new information on the chemistry, analytical chemistry, toxicology, molecular modeling, and liver tumor promotion of the toxins. Microcystis species are described in relation to morphological features, allozyme genotype, and toxin content. Seasonal changes of Microcystis population are described with special references to toxic species and composition of the toxins. Chemical characteristics of microcystins are reviewed and the process for identification of microcystins is described.