Invertebrate and Fish Tissue Culture

Invertebrate and Fish Tissue Culture
Author: Yukiaki Kuroda
Publisher: Springer
Total Pages: 316
Release: 1988-06-09
Genre: Medical
ISBN:

The sessions dealt with new cultures and techniques, physiology and endocrinology of cells in vitro, production of specific proteins in vitro, cell differentiation and gene expression, application of cell cultures to pathology and microbiology, fish cell culture development and application, and new cell lines and their characterization.

Program Issue

Program Issue
Author: Tissue Culture Association
Publisher:
Total Pages: 184
Release: 1991
Genre:
ISBN:

Invertebrate and Fish Tissue Culture

Invertebrate and Fish Tissue Culture
Author: Yukiaki Kuroda
Publisher: Springer
Total Pages: 316
Release: 1988
Genre: Science
ISBN:

The sessions dealt with new cultures and techniques, physiology and endocrinology of cells in vitro, production of specific proteins in vitro, cell differentiation and gene expression, application of cell cultures to pathology and microbiology, fish cell culture development and application, and new cell lines and their characterization.

Baculovirus Expression Protocols

Baculovirus Expression Protocols
Author: Christopher D. Richardson
Publisher: Springer Science & Business Media
Total Pages: 412
Release: 2008-02-02
Genre: Science
ISBN: 159259526X

Baculovirus Expression Protocols offers both industrial and university-based researchers a comprehensive comilation of the latest baculovirus techniques along with step-by-step instructions and time-saving techniques. The contributors-leading authorities in the field-present the assorted expression plasmids currently in use, guide the reader through the process of generating and selecting recombinant virus, and describe specific examples of recombinant protein production and purification. The emphasis is on alternative and simpler screening techniques for the selection of recombinant baculovirus. The book also surveys the various insect cell lines currently compatible with the baculovirus system. Highlights include production of recombinant virus using linearized DNA and vectors that contain a b-galactosidase indicator and a complete list of expression vectors currently available. Detailed descriptions for the scale-up of protein production using spinner flasks, bioreactors, and insect larvae are also included. Baculovirus Expression Protocols offers both industrial and university-based researchers an outstanding collection of reproducible, step-by-step laboratory protocols. It will immediately become indispensable for anyone working with baculoviruses and their application in the expression of recombinant proteins in insect cells.