Preparative Bioaffinity Chromatography
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Author | : J. Turková |
Publisher | : Elsevier |
Total Pages | : 819 |
Release | : 1993-09-14 |
Genre | : Science |
ISBN | : 0080858635 |
Bioaffinity chromatography is now the preferred choice for the purification, determination or removal of many biologically active substances. The book includes information on biologically active substances with their affinants, solid supports and methods of coupling, summarized in tables covering classical, high-performance liquid and large-scale bioaffinity chromatography.Optimization of the preparation and the use of highly active and stable biospecific adsorbents is discussed in several chapters. Following a chapter dealing with the choice of affinity ligands, affinity-sorbent bonding is described in detail. Other chapters give information on solid supports, the most common coupling procedures and a general discussion of sorption and elution. Several applications of bioaffinity chromatography are described, e.g. quantitative evaluation of biospecific complexes and many applications in medicine and in the biotechnology industry.
Author | : Ton van Sommeren |
Publisher | : |
Total Pages | : 119 |
Release | : 1997 |
Genre | : |
ISBN | : |
Author | : Hassan Y. Aboul-Enein |
Publisher | : CRC Press |
Total Pages | : 482 |
Release | : 2020-10-15 |
Genre | : Science |
ISBN | : 1000146251 |
Reports up-to-date research developments on purifying and isolation large organic molecules. The text provides information on high-performance liquid chromatography and capillary electrophoresis (CE) as tools for analyzing biomacromolecules and developing new biochemical and medicinal compounds. It applies biochemical separation technology to the study of macromolecules such as proteins, polysaccharides, nucleic acids and more.
Author | : Irwin M. Chaiken |
Publisher | : CRC Press |
Total Pages | : 303 |
Release | : 2018-01-10 |
Genre | : Science |
ISBN | : 135108657X |
This volume presents discussions of theoretical and experimental considerations that have led to the analytical affinity chromatography field, as well as current efforts to use this methodology to characterize the interaction mechanisms of biological macromolecules and to establish conditions for employing bioaffinity chromatographic systems as preparative tools. The chapters include a comprehensive discussion of interactive chromatography theory (DeLisi and Hethcote), a review of experimental data obtained for biological macromolecules and the relevant theoretical considerations of affinity chromatography which led to them (Swaisgood and Chaiken), an evaluation of rate processes in affinity chromatography and the potential to determine biologically meaningful chemical rate constants (Walters), and the use of quantitative and molecular considerations to design affinity chromatographic systems (Stellwagen and Liu).
Author | : Irwin Chaiken |
Publisher | : Elsevier |
Total Pages | : 542 |
Release | : 2012-12-02 |
Genre | : Science |
ISBN | : 0323146627 |
Affinity Chromatography and Biological Recognition contains manuscripts presented at the Fifth International Symposium on Affinity Chromatography and Biological Recognition convened in June 12-17, 1983, at St. John's College in Annapolis, Maryland. Organized into six parts encompassing 82 chapters, the book begins by examining the growing synergism between affinity methods and the understanding and study of basic principles of biological recognition. The book then focuses on the trends and progress in the design and application of affinity methods for isolation, therapeutics, diagnostics, and biotechnology. Significant chapters are devoted to the contributions of affinity methodology in such areas as cell membrane receptors, quantitative properties of macromolecular interactions, microscale analytical and preparative applications of high performance affinity chromatography, antibodies as in vivo and in vitro diagnostic and therapeutic agents, and drug targeting. This volume will be a stimulus for broad and creative application of affinity concepts and methods in many fields of biomedical research and biotechnology.
Author | : J. Turková |
Publisher | : Elsevier |
Total Pages | : 417 |
Release | : 1978-01-01 |
Genre | : Science |
ISBN | : 0080858120 |
Bioaffinity chromatography has increasingly become the method of choice for the purification, determination or removal of many biologically active substances.
Author | : Donald Huddler |
Publisher | : John Wiley & Sons |
Total Pages | : 148 |
Release | : 2017-10-02 |
Genre | : Science |
ISBN | : 111909948X |
Applied Biophysics for Drug Discovery is a guide to new techniques and approaches to identifying and characterizing small molecules in early drug discovery. Biophysical methods are reasserting their utility in drug discovery and through a combination of the rise of fragment-based drug discovery and an increased focus on more nuanced characterisation of small molecule binding, these methods are playing an increasing role in discovery campaigns. This text emphasizes practical considerations for selecting and deploying core biophysical method, including but not limited to ITC, SPR, and both ligand-detected and protein-detected NMR. Topics covered include: • Design considerations in biophysical-based lead screening • Thermodynamic characterization of protein-compound interactions • Characterizing targets and screening reagents with HDX-MS • Microscale thermophoresis methods (MST) • Screening with Weak Affinity Chromatography • Methods to assess compound residence time • 1D-NMR methods for hit identification • Protein-based NMR methods for SAR development • Industry case studies integrating multiple biophysical methods This text is ideal for academic investigators and industry scientists planning hit characterization campaigns or designing and optimizing screening strategies.
Author | : International Symposium on Bioaffinity Chromatography and Related Techniques |
Publisher | : |
Total Pages | : |
Release | : 1985 |
Genre | : |
ISBN | : |
Author | : Arne Staby |
Publisher | : John Wiley & Sons |
Total Pages | : 608 |
Release | : 2017-02-02 |
Genre | : Technology & Engineering |
ISBN | : 1119031176 |
Preparative Chromatography for Separation of Proteins addresses a wide range of modeling, techniques, strategies, and case studies of industrial separation of proteins and peptides. • Covers broad aspects of preparative chromatography with a unique combination of academic and industrial perspectives • Presents Combines modeling with compliantce useing of Quality-by-Design (QbD) approaches including modeling • Features a variety of chromatographic case studies not readily accessible to the general public • Represents an essential reference resource for academic, industrial, and pharmaceutical researchers
Author | : |
Publisher | : |
Total Pages | : 0 |
Release | : 1988 |
Genre | : |
ISBN | : |