Plant Tissue Culture Theory Practicals 2nd Ed
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| Author | : T. Pullaiah |
| Publisher | : Scientific Publishers |
| Total Pages | : 249 |
| Release | : 2017-03-01 |
| Genre | : Technology & Engineering |
| ISBN | : 9387869806 |
Plant Tissue Culture, Second Edition is accompanied with new exercises demonstrating new arrays along with information on development of a customized protocol for protoplast isolation, suspension, haploid cultures, secondary metabolite production, and cryopreservation techniques. All experimental systems are written clear and easy-to-understand manner with the text being well-documented along with detailed drawings containing the plant tissue culture requirements for each particular application. Besides addressing recent advancements on wide variety of topics of Plant Tissue Culture, it gives the practical and technical knowledge required to train the next generation of plant scientists regardless of their ultimate specialization. It includes the complements of both theory and experiments. Plant Scientists, teachers and students will benefit greatly from this clearly presented tissue culture techniques that guides reader from lab setup to supplies, stock solution and media preparation, measurements, explant selection and disinfestations, along with their experimental observations.
| Author | : Robert H. Smith |
| Publisher | : Academic Press |
| Total Pages | : 190 |
| Release | : 2012-12-02 |
| Genre | : Science |
| ISBN | : 0323160476 |
Plant Tissue Culture Techniques and Experiments is a manual that contains laboratory exercises about the demonstration of the methods and different plant materials used in plant tissue culture. It provides an overview on the plant cell culture techniques and plant material options in selecting the explant source. This book starts by discussing the proper setup of a tissue culture laboratory and the selection of the culture medium. It then explains the determination of an explant which is the ultimate goal of the cell culture project. The explant is a piece of plant tissue that is used in tissue culture. Furthermore, the book discusses topics about callus induction, regeneration and morphogenesis process, and haploid plants from anther and pollen culture. The meristem culture for virus-free plants and in vitro propagation for commercial propagation of ornamentals are also explained in this manual. The book also provides topics and exercises on the protoplast isolation and fusion and agrobacterium-mediated transformation of plants. This manual is intended for college students, both graduate and undergraduate, who study chemistry, plant anatomy, and plant physiology.
| Author | : S.S. Bhojwani |
| Publisher | : Elsevier |
| Total Pages | : 779 |
| Release | : 1996-11-08 |
| Genre | : Science |
| ISBN | : 0080539092 |
Since the publication of the first edition in 1983, several new and exciting developments have taken place in the field of plant tissue culture, which forms a major component of what is now called plant biotechnology. The revised edition presents updated information on theoretical, practical and applied aspects of plant tissue culture. Each chapter has been thoroughly revised and, as before, is written in lucid language, includes relevant media protocols, and is profusely illustrated with self-explanatory diagrams and original photographs. This book includes three new chapters: "Variant selection", "Genetic Engineering" and "Production of Industrial Compounds" and contains a complete bibliography and a glossary of terms commonly used in tissue culture literature.This updated version proves to be an excellent text for undergraduate, postgraduate students and teachers in various fields of plant sciences and a useful reference book for those interested in the application of any aspect of this aseptic technology.
| Author | : Sant Saran Bhojwani |
| Publisher | : Springer Science & Business Media |
| Total Pages | : 318 |
| Release | : 2013-03-20 |
| Genre | : Science |
| ISBN | : 8132210263 |
Plant tissue culture (PTC) is basic to all plant biotechnologies and is an exciting area of basic and applied sciences with considerable scope for further research. PTC is also the best approach to demonstrate the totipotency of plant cells, and to exploit it for numerous practical applications. It offers technologies for crop improvement (Haploid and Triploid production, In Vitro Fertilization, Hybrid Embryo Rescue, Variant Selection), clonal propagation (Micropropagation), virus elimination (Shoot Tip Culture), germplasm conservation, production of industrial phytochemicals, and regeneration of plants from genetically manipulated cells by recombinant DNA technology (Genetic Engineering) or cell fusion (Somatic Hybridization and Cybridization). Considerable work is being done to understand the physiology and genetics of in vitro embryogenesis and organogenesis using model systems, especially Arabidopsis and carrot, which is likely to enhance the efficiency of in vitro regeneration protocols. All these aspects are covered extensively in the present book. Since the first book on Plant Tissue Culture by Prof. P.R. White in 1943, several volumes describing different aspects of PTC have been published. Most of these are compilation of invited articles by different experts or proceedings of conferences. More recently, a number of books describing the Methods and Protocols for one or more techniques of PTC have been published which should serve as useful laboratory manuals. The impetus for writing this book was to make available a complete and up-to-date text covering all basic and applied aspects of PTC for the students and early-career researchers of plant sciences and plant / agricultural biotechnology. The book comprises of nineteen chapters profusely illustrated with self-explanatory illustrations. Most of the chapters include well-tested protocols and relevant media compositions that should be helpful in conducting laboratory experiments. For those interested in further details, Suggested Further Reading is given at the end of each chapter, and a Subject and Plant Index is provided at the end of the book.
| Author | : J.K. Maheshwari |
| Publisher | : Scientific Publishers |
| Total Pages | : 470 |
| Release | : 1996 |
| Genre | : Science |
| ISBN | : 9387991202 |
The present volume contains an editorial review article New vistas in Ethnobotany along with 76 other articles written by eminent ethno-botanist working in various scientific research and academic institutions in South Asia. Ethnobotany of tribals/traditional uses of plants in different parts of South Asia and ethnobotanical uses of Herbarium have been dealt with in this work besides many other useful articles. This work provides a glimpse of rich ethnobotanical heritage of South Asia.
| Author | : Sunghun Park |
| Publisher | : Academic Press |
| Total Pages | : 254 |
| Release | : 2021-02-17 |
| Genre | : Science |
| ISBN | : 0323851371 |
Plant Tissue Culture: Techniques and Experiments, Fourth Edition, builds on the classroom tested, audience proven manual that has guided users through successful plant culturing for almost 30 years. The book's experiments demonstrate major concepts and can be conducted with a variety of plant materials readily available throughout the year. This fully updated edition describes the principles of the newest technologies, including CRISPR/Cas9 gene editing and RNAi technology with plant cell and tissue cultures and their applications. Bridging the gap between theory and practice, this book contains detailed methodology supported by comprehensive illustrations, giving users a diverse learning experience for both university students and plant scientists. - Provides fundamental principles, methods and techniques in plant cell, tissue and organ culture that can be applied to all crop plants, including agronomic crops, horticulture and forestry crops for germplasm improvement - Guides readers from lab setup to supplies, stock solution and media preparation, explant selection and disinfestations, and experimental observations and measurement - Contains the latest advances and updates since the previous edition published in 2012
| Author | : John H. Dodds |
| Publisher | : International Potato Center |
| Total Pages | : 260 |
| Release | : 1985-10-31 |
| Genre | : Science |
| ISBN | : 9780521315166 |
The second edition of Experiments in Plant Tissue Culture makes available new information that has resulted from recent advances in the applications of plant tissue culture techniques to agriculture and industry. This comprehensive laboratory text takes the reader through a graded series of experimental protocols and also provides an introductory review of each topic. Topics include: a plant tissue culture laboratory, aseptic techniques, nutritional components of media, callus induction, organ formation, xylem cell differentiation, root cultures, cell suspensions, micropropagation, embryogenesis, isolation and fusion of protoplasts, haploid cultures, storage of plant genetic resources, secondary metabolite production, and quantification of procedures. This volume offers all of the basic experimental methods for the major research areas of plant tissue culture, and it will be invaluable to undergraduates and research investigators in the plant sciences.
| Author | : B.D. Singh |
| Publisher | : Scientific Publishers |
| Total Pages | : 544 |
| Release | : 2019-05-01 |
| Genre | : Science |
| ISBN | : 9389061288 |
The discipline of plant breeding has undergone transformation due to the assimilation of the rapid developments in molecular biology. The existing books on plant breeding deal mainly with the classical approaches, while specialized books on molecular approaches usually lack discussion of the classical methods. The book Plant Breeding for 21st Century attempts to present the complete picture of plant breeding ranging from the classical to the molecular approaches applied to crop improvement. The book is divided into four sections: Classical Plant Breeding, Transgenic technology, Molecular Markers, and Miscellaneous. The first section deals with the classical plant breeding and is divided into eight chapters. The second section has four chapters and describes transgenic technology. The third section discusses various aspects of molecular markers and is spread over three chapters. The final section has a single chapter dealing with variety release, seed multiplication and intellectual property rights. This book is designed primarily for graduate students, viz., B.Sc. agriculture and B.Sc. science students with botany as one of the subjects, who would get their first exposure to plant breeding. It would also be useful for the post-graduate students, especially in botany, and to teachers of the subject. The book is written in simple and easy to understand language. Illustrations and photographs have been provided wherever they were expected to facilitate comprehension of the subject under discussion.
| Author | : K. R. Aneja |
| Publisher | : New Age International |
| Total Pages | : 636 |
| Release | : 2007 |
| Genre | : Microbiology |
| ISBN | : 9788122414943 |
Microorganisms Are Living Things Like Plants And Animals But Because Of Their Minute Size And Omnipresence, Performing Experiments With Microbes Requires Special Techniques And Equipment Apart From Good Theoretical Knowledge About Them. This Easy To Use Revised And Updated Edition Provides Knowledge About All The Three I.E., Techniques, Equipment And Principles Involved.The Notable Feature Of This Edition Is The Addition Of New Sections On Bacterial Taxonomy That Deals With The Criteria Used In Identification, Phylogeny And Current System Of Classification Of Procaryotes Based On The Second Edition Of Bergey Manual Of Systematic Bacteriology And The Section One On History Of Discovery Of Events That Covers Chronologically Important Events In Microbiology With The Contribution Of Pioneer Microbiologists Who Laid The Foundation Of The Science Of Microbiology. In The Subsequent Twenty-Two Sections, Various Microbiological Techniques Have Been Described Followed By Several Experiments Illustrating The Properties Of Microorganisms And Highlighting Their Involvement In Practically Every Sphere Of Life.Along With The Cultivation/Isolation/Purification Of Microbes, This Edition Also Contains Exercises Concerning Air, Soil, Water, Food, Dairy And AgriculturalMicrobiology, Bacterial Genetics, Plant Pathology, Plant Tissue Culture And Mushroom Production Technology. This Manual Contains 163 Experiments Spread Over 22 Different Sections. The Exercises Are Presented In A Simple Language With Explanatory Diagrams And A Brief Recapitulation Of Their Theory And Principle.The Exercises Are Selected By Keeping In Mind The Easy Availability Of Cultures, Culture Media And Equipment. Appendices At The End Of The Manual Provide A Reference To The Source For Obtaining Cultures Of Microbes, Culture Media And Preparation Of Various Stains, Reagents And Media In The Laboratory And Classification Of Procaryotes According To The First And Second Editions Of Bergey Is Manual Of Systematic Bacteriology.This Book Would Be Useful For The Undergraduate And Postgraduate Students, Teachers And Scientists In Diverse Areas Including The Biological Sciences, The Allied Health Services, Environmental Science, Biotechnology, Agriculture, Nutrition, Pharmacy And Various Other Professional Programmes Like Milk Processing Units, Diagnostic (Clinical) Microbiological Laboratories And Mushroom Cultivation At Small Or Large Scales.
| Author | : Roberta H. Smith |
| Publisher | : Academic Press |
| Total Pages | : 206 |
| Release | : 2013 |
| Genre | : Science |
| ISBN | : 0124159206 |
Plant Tissue Culture, Third Edition builds on the classroom tested, audience proven manual that has guided users through successful plant culturing A.tumefaciens mediated transformation, infusion technology, the latest information on media components and preparation, and regeneration and morphogenesis along with new exercises and diagrams provide current information and examples. The included experiments demonstrate major concepts and can be conducted with a variety of plant material that are readily available throughout the year. This book provides a diverse learning experience and is appropriate for both university students and plant scientists. Provides new exercises demonstrating tobacco leaf infiltration to observe transient expression of proteins and subcellular location of the protein, and information on development of a customized protocol for protoplast isolation for other experimental systems Includes detailed drawings that complement both introductions and experiments Guides reader from lab setup to supplies, stock solution and media preparation, explant selection and disinfestations, and experimental observations and measurement Provides the latest techniques and media information, including A. tumefaciens mediated transformation and infusion technology Fully updated literature
