Plant Lipid Signaling Protocols
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| Author | : Teun Munnik |
| Publisher | : Springer Nature |
| Total Pages | : 298 |
| Release | : 2013-05-17 |
| Genre | : Science |
| ISBN | : 1627034013 |
As scientist begin to understand the complexity of lipid signaling and its roles in plant biology, there is an increasing interest in their analysis. Due to the low abundancy and transient nature of some of these hydrophobic compounds, this is not always easy. In Plant Lipid Signaling Protocols, expert researchers in the field detail experimental approaches by which plant signaling lipids can be studied. These methods and techniques include analysis of plant signaling lipids, including detailed protocols to detect various relevant compounds by targeted or non-targeted approaches; to assay relevant enzyme activities in biological material or using recombinant enzymes; to test for specific binding of signaling lipids to protein partners; or to visualize signaling lipids or lipid-derived signals in living plant cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Plant Lipid Signaling Protocols aids plant researchers in the continuing to study the roles of lipid signals.
| Author | : Dorothea Bartels |
| Publisher | : Humana |
| Total Pages | : 472 |
| Release | : 2022-06-12 |
| Genre | : Science |
| ISBN | : 9781071613641 |
This volume explores analytical methods to study complex lipid mixtures from plants and algae. The chapters in this book are organized into five parts and cover topics such as basic methods of lipid isolation and analysis; mass spectrometry and NMR analysis; lipid isolation and analysis from plant tissues, cell compartments and organelles; lipid signaling, lipid-protein interactions, and imaging; and lipid databases. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Plant Lipids: Methods and Protocols is a valuable guide for experienced researchers and undergraduate, graduate, and Ph.D. students. This book is also an excellent resource for novice scientists with little to no experience in lipid experiments who are interested in approaching this field experimentally.
| Author | : Xuemin Wang |
| Publisher | : Frontiers E-books |
| Total Pages | : 249 |
| Release | : 2013-07-30 |
| Genre | : |
| ISBN | : 2889191486 |
Cell membranes are the initial and focal sites of stimulus perception and signal transduction. Membrane lipids are rich sources for the production of signaling messengers that mediate plant growth, development, and response to nutrient status and stresses. In recent years, substantial progress has been made toward understanding lipid signaling in plants, but many fundamental questions remain: What lipids are signaling messengers or mediators in plants? How are the signaling lipids produced and metabolized? In what plant cellular and physiological processes are various lipid mediators involved? How do they carry out their signaling functions? How do lipid signaling networks contribute to modulating plant growth, development, and responses to hormones and stresses? In this Research Topic issue, we invite the broad plant community to address the above questions.Cell membranes are the initial and focal sites of stimulus perception and signal transduction. Membrane lipids are rich sources for the production of signaling messengers that mediate plant growth, development, and response to nutrient status and stresses. In recent years, substantial progress has been made toward understanding lipid signaling in plants, but many fundamental questions remain: What lipids are signaling messengers or mediators in plants? How are the signaling lipids produced and metabolized? In what plant cellular and physiological processes are various lipid mediators involved? How do they carry out their signaling functions? How do lipid signaling networks contribute to modulating plant growth, development, and responses to hormones and stresses? In this Research Topic issue, we invite the broad plant community to address the above questions.
| Author | : Teun Munnik |
| Publisher | : Springer Science & Business Media |
| Total Pages | : 332 |
| Release | : 2009-12-03 |
| Genre | : Science |
| ISBN | : 3642038735 |
This comprehensive update on plant lipid signaling covers the measurement, regulation and function of phospholipases, lipid kinases, lipid phosphatases, inositolpolphosphates, polyphosphoinositides, phosphatic acid, and other lipid signals such as oxylipins.
| Author | : Banafshé Larijani |
| Publisher | : Humana Press |
| Total Pages | : 0 |
| Release | : 2014-10-30 |
| Genre | : Science |
| ISBN | : 9781627038829 |
Lipid Signaling Protocols assembles in a single volume the various tools and methodologies needed by the interested investigator to unravel lipid dependent signaling and cell function. Divided into two convenient sections, the volume begins by summarizing the physical properties of hydrophobic metabolites as well as the physical methodologies used for their analysis, which leads to the second section and its selection of biological methods, focused around the most relevant lipids, their corresponding metabolizing enzymes and the recognition proteins. Following the highly successful Methods in Molecular BiologyTM series format, the chapters provide readily reproducible laboratory protocols, lists of necessary materials and reagents, and the tips on troubleshooting and avoiding known pitfalls. Contributed to by top researchers in the field, Lipid Signaling Protocols is an essential resource for both experienced and novice researchers who desire a better understanding of the application of physical methodologies in the context of lipid signaling and lipid metabolism in cell biology.
| Author | : Gregory A. Tucker |
| Publisher | : Springer Science & Business Media |
| Total Pages | : 201 |
| Release | : 2008-02-04 |
| Genre | : Science |
| ISBN | : 1592590675 |
Established investigators from around the world describe in step-by-step detail their best techniques for the study of plant hormones and their regulatory activities. These state-of-the-art methods include contemporary approaches to identifying the biosynthetic pathways of plant hormones, monitoring their levels, characterizing the receptors with which they interact, and analyzing the signaling systems by which they exert their effects. Comprehensive and fully detailed for reproducible laboratory success, Plant Hormone Protocols offers plant biologists an indispensable compendium of today's most powerful methods and strategies to studying plant hormones, their regulation, and their activities.
| Author | : Jose R. Botella |
| Publisher | : Humana Press |
| Total Pages | : 241 |
| Release | : 2016-08-23 |
| Genre | : Science |
| ISBN | : 9781493949595 |
This fully updated volume reflects the spectacular advances in our knowledge of signal transduction pathways with a selection of ‘classic’ as well as newly developed approaches. These detailed approaches expand into the fields of molecular biology, biochemistry, physiology, cell biology, genetics, and genomics. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Practical and up-to-date, Plant Signal Transduction: Methods and Protocols, Second Edition serves as an ideal guide for researchers exploring the vast array of signals produced by plants to ensure their survival.
| Author | : J.C. Kader |
| Publisher | : Springer Science & Business Media |
| Total Pages | : 598 |
| Release | : 2013-04-18 |
| Genre | : Science |
| ISBN | : 9401583943 |
A collection of papers that comprehensively describe the major areas of research on lipid metabolism of plants. State-of-the-art knowledge about research on fatty acid and glycerolipid biosynthesis, isoprenoid metabolism, membrane structure and organization, lipid oxidation and degradation, lipids as intracellular and extracellular messengers, lipids and environment, oil seeds and gene technology is reviewed. The different topics covered show that modern tools of plant cellular and molecular biology, as well as molecular genetics, have been recently used to characterize several key enzymes of plant lipid metabolism (in particular, desaturases, thioesterases, fatty acid synthetase) and to isolate corresponding cDNAs and genomic clones, allowing the use of genetic engineering methods to modify the composition of membranes or storage lipids. These findings open fascinating perspectives, both for establishing the roles of lipids in membrane function and intracellular signalling and for adapting the composition of seed oil to the industrial needs. This book will be a good reference source for research scientists, advanced students and industrialists wishing to follow the considerable progress made in recent years on plant lipid metabolism and to envision the new opportunities offered by genetic engineering for the development of novel oil seeds.
| Author | : Ye Xia |
| Publisher | : |
| Total Pages | : 139 |
| Release | : 2010 |
| Genre | : |
| ISBN | : |
| Author | : Thomas Pfannschmidt |
| Publisher | : Humana Press |
| Total Pages | : 360 |
| Release | : 2009-03-23 |
| Genre | : Science |
| ISBN | : 9781607610694 |
During recent years, research has greatly expanded our understanding of the sophisticated molecular network of responses which enable plants to develop, survive and propagate under a wide range of conditions. In Plant Signal Transduction: Methods and Protocols, an international panel of experts provide well-established methods vital to analyzing plant signal transduction on the molecular level. Featuring experimental procedures on several of the most popular model organisms, the volume focuses on in planta analyses and the proteins involved in signal transduction in order to aid with the establishment of laboratory techniques or the modification of the protocols for other plants. As part of the highly successful Methods in Molecular BiologyTM series, the chapters include brief introductions to the subject, lists of necessary materials, readily reproducible laboratory protocols, and tips on trouble-shooting and avoiding known pitfalls. Comprehensive and cutting-edge, Plant Signal Transduction: Methods and Protocols will benefit plant scientists wishing to improve their experimental approaches and delve further into this exciting and important field of study
